Changes of microglial activation after heat stroke in mice brain

Gong Wang; Jun Liu; Genlin He; Ju Yang; Ping Li; Xiaoliang Zhang; Wenjing Li; Zhen Luo; Tingting Shen; Xuesen Yang

doi:10.16016/j.1000-5404.201610042

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Changes of microglial activation after heat stroke in mice brain

更新时间：2026-01-09

- Changes of microglial activation after heat stroke in mice brain
- J Third Mil Med Univ Vol. 39, Issue 4, Pages: 317-322(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81472952);the Military Medical Pre-research Foundation of Third Military Medical University(2014XJY03)
- DOI：10.16016/j.1000-5404.201610042
  CLC：
- Received：09 October 2016，
  
  Published：28 February 2017
- 稿件说明：
移动端阅览
Gong Wang, Jun Liu, Genlin He, et al. Changes of microglial activation after heat stroke in mice brain[J]. J Third Mil Med Univ, 2017, 39(4): 317-322.
DOI：

Gong Wang, Jun Liu, Genlin He, et al. Changes of microglial activation after heat stroke in mice brain[J]. J Third Mil Med Univ, 2017, 39(4): 317-322. DOI： 10.16016/j.1000-5404.201610042.

摘要

Abstract

Objective

To explore the role of microglia played in mice brain after heat stroke by observing the effect of acute injury-related heat exposure on microglial activation.

Method

Fifty-seven adult male BALB/c mice were randomly divided into normal control group and 3 heat stroke groups after heat exposure of different time periods (1

and 24 h). The heat stroke groups were established by placing the mice in small animal environmental stimulation chambers with air temperature of 41.2±0.5 ℃ and relative humidity of （60±2）%.M1 polarization markers including TNF-α

CD45

and CD11b as well as M2 polarization markers including Arg1

FIZZ

and CD206 were measured using real-time reverse transcriptasc-polymerase chain reaction (RT-PCR). Protein expression of CD45

CD11b

FIZZ

and CD206 was detected by Western blotting. Distribution and co-localization of CD45 and CD206 were assessed by immunofluorescence staining.

Results

RT-PCR analysis showed that the mRNA levels of M1 polarization markers (TNF-α

CD45

and CD11b) and M2 polarization markers (Arg1

FIZZ

and CD206) reached the peak at 1 and 24 h after heat exposure(

0.01)

respectively. Western blot results revealed that the expression of M1 polarization markers CD45 and CD11b was significantly increased after 1 h followed by gradually declining to the base level 24 h after heat exposure. M2 polarization markers FIZZ and CD206 showed different expression trends

but both of their expression were significantly increased and reached the maximum at 24 h (

0.01). Immunofluorescence staining detected the co-localization of CD45 and CD206

exhibiting strong fluorescence signals at 1 and 24 h

respectively

after heat exposure in the cerebral cortex of mice.

Conclusion

Microglia are significantly activated in response to brain injury after heat exposure

which is characterized by prominent polarization of M1 at 1 h and M2 at 24 h after heat stroke. This polarized profile of microglia may be associated with central nervous system injury after heat stroke.

关键词

Keywords

references

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