Preliminary observation of heart, lung, liver and kidney injury in rats induced by smoke inhalation in military airtight cabins

Lei Sun; Sheng Chen; Ying Cai; Zhihong Cui; Wenbin Liu; Chengyu Tao; Jinyi Liu; Jia Cao

doi:10.16016/j.1000-5404.201610035

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Preliminary observation of heart, lung, liver and kidney injury in rats induced by smoke inhalation in military airtight cabins

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- Preliminary observation of heart, lung, liver and kidney injury in rats induced by smoke inhalation in military airtight cabins
- Journal of Third Military Medical University Vol. 39, Issue 6, Pages: 499-503(2017)
- 作者机构：
  
  400038 重庆，第三军医大学军事预防医学院毒理学研究所
- 作者简介：
- 基金信息：
  
  the Mandatory Project of Applied Research on Health and Disease Control of PLA(13BJYZ28)
- DOI：10.16016/j.1000-5404.201610035
  CLC： R-332; R363.374; R644
- Received：08 October 2016，
  
  Revised：2016-12-26，
  
  Published：2017-06
- 稿件说明：
移动端阅览
Lei Sun, Sheng Chen, Ying Cai, et al. Preliminary observation of heart, lung, liver and kidney injury in rats induced by smoke inhalation in military airtight cabins[J]. Journal of Third Military Medical University, 2017, 39(6): 499-503.
DOI：

Lei Sun, Sheng Chen, Ying Cai, et al. Preliminary observation of heart, lung, liver and kidney injury in rats induced by smoke inhalation in military airtight cabins[J]. Journal of Third Military Medical University, 2017, 39(6): 499-503. DOI： 10.16016/j.1000-5404.201610035.

摘要

目的

探讨军用密闭舱室非金属材料燃烧释放的有毒烟雾对雄性大鼠心、肺、肝、肾损伤的特点。

方法

建立密闭舱室内动物烟雾吸入模型，将36只雄性SD大鼠按随机数字表法分为对照组及烟雾吸入后1、6、24、72 h和7 d组，对照组动物于染毒箱内自由呼吸新鲜空气10 min，染毒组动物于染毒箱内连续吸入烟雾10 min，观察烟雾吸入后动物的中毒症状、血压、心率、血气、血清生化等指标。

结果

烟雾吸入后，动物立刻出现呼吸节律改变、呼吸困难等CO中毒表现；烟雾吸入后1 h大鼠心率显著下降 (

0.05)，pCO

显著升高 (

0.05)，pO

显著降低 (

0.05)；烟雾吸入后1~72 h组动物谷丙转氨酶 (ALT) 均显著升高 (

0.05)，其中6 h时达峰值 (

0.01)；烟雾吸入后各组AST、LDH、α-HBDH、CK及CK-MB等心肌酶谱指标均持续升高，其中1 h组各指标与对照组比较差异均有统计学意义 (

0.05)。

结论

密闭舱室火灾烟雾吸入首先对大鼠的肺脏造成原发性损伤，心、肝、肾产生继发性的功能损害，且上述功能损害主要发生在烟雾吸入后“24 h”窗口期内，其后由于机体代偿可逐渐恢复至正常。

Abstract

Objective

To investigate the characteristics of rat heart

lung

liver and kidney injury induced by toxic smoke released from combustion of non-metal materials in military airtight cabins.

Methods

On the basis of animal smoke inhalation model established in our laboratory

36 male SD rats were divided randomly into control group and 5 inhalation groups (1

24 and 72 h

and 7 d after smoke exposure)

and the clinical symptoms of poisoning

blood pressure

heart rate

blood gas

and serum biochemical indexes were observed after smoke inhalation.

Results

After smoke inhalation

the symptoms of CO poisoning

such as respiratory rhythm change and breathing difficulties

were observed immediately. Compared with the control group

the heart rate was decreased in the rats exposed to the smoke

pCO

increased

and pO

declined significantly at 1 h after exposure (

0.05). The level of alanine aminotransferase (ALT) was increased significantly in 1~72 h after exposure (

0.05) and reached a peak at 6 h after exposure (

0.01). The levels of AST

LDH

α-HBDH

CK and CK-MB were increased constantly in the rats after smoke inhalation

and all of the indexes above were elevated significantly in the group of 1 h post-exposure as compared with the control group (

0.05).

Conclusion

Smoke inhalation in military airtight cabins can cause primary injury to the lungs and secondary injury to the heart

liver and kidneys mainly within 24 h after exposure

and the body can gradually recover to the normal state because of compensation.

关键词

Keywords

references

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