Inflammatory responses of central and peripheral nerve system  in early recovery stage of heat stroked mice

Jun Liu; Gong Wang; Genlin He; Ju Yang; Xiaoliang Zhang; Zhen Luo; Tingting Shen; Ping Li; Wenjing Li; Xuesen Yang

doi:10.16016/j.1000-5404.201609189

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Inflammatory responses of central and peripheral nerve system in early recovery stage of heat stroked mice

更新时间：2026-01-09

- Inflammatory responses of central and peripheral nerve system in early recovery stage of heat stroked mice
- J Third Mil Med Univ Vol. 39, Issue 4, Pages: 311-316(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Key Project of “Twelfth Five-year Plan" for Military Logistic Research(BWS14J023);the Military Medical Pre-research Foundation of Third Military Medical University(2014XJY03)
- DOI：10.16016/j.1000-5404.201609189
  CLC：
- Received：28 September 2016，
  
  Published：28 February 2017
- 稿件说明：
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Jun Liu, Gong Wang, Genlin He, et al. Inflammatory responses of central and peripheral nerve system in early recovery stage of heat stroked mice[J]. J Third Mil Med Univ, 2017, 39(4): 311-316.
DOI：

Jun Liu, Gong Wang, Genlin He, et al. Inflammatory responses of central and peripheral nerve system in early recovery stage of heat stroked mice[J]. J Third Mil Med Univ, 2017, 39(4): 311-316. DOI： 10.16016/j.1000-5404.201609189.

摘要

Abstract

Objective

To investigate the change and correlation of inflammatory response between the central and peripheral nerve system in early recovery stage (0~24 h)of mice after heat stroke(HS).

Method

A total of 70 BALB/c male mice were randomly divided into control (CON) group and other 5 different phase-group after HS (HS-0 h

HS-1 h

HS-6 h

HS-12 h

and HS-24 h). HS mouse model was established using environmental stimulation chamber. The survival

rectal temperature

and body weight loss were measured. The change of tumor necrosis factor alpha (TNF-α)

interleukin (IL)-1β

and IL-6 in the cortex and hippocampus was determined by quantitative real-time polymerase chain reaction(PCR). The serum levels of TNF-α

IL-1β

and IL-6 was measured by enzyme-linked immunosorbent assay (ELISA). The endotoxin concentration in the serum was detected by Kinetic Turbidimetric Tachypleus Amebocyte Lysate.

Results

The rectal temperature in mice exceeded 42 ℃ after heat exposure.Hypothermia occurred in 1~2 h and the survival rate of mice was 78.57%after 24 h. These suggested that HS mouse model was successfully generated. Compared to the control group

the mRNA expression of TNF-α

IL-1β

and IL-6 in the cerebral cortex and hippocampus of HS groups was increased rapidly

reaching the maximum at 1 h (

0.01). The expression was then gradually decreased after 6 h and approached to the normal level at 24 h (

0.05).At 6 h

the expression of these inflammatory factors was still higher than the CON group (

0.01).The expression levels of TNF-α

IL-1β

and

IL-6 in the peripheral serum were increased significantly at 1 h

then decreased. At 24 h

the levels still remained higher than the CON group (

0.01). The concentration of endotoxin in the serum was elevated at 0 h

reached the peak at 6 h and maintained the high secretion level until 24 h (

0.01).

Conclusion

The secretion of inflammatory factors in central nerve system response to heat exposure is transient within 12 h

while is persistent to 24 h in peripheral inflammatory secretion.The different inflammation response between the central and peripheral nerve system after heat stroke implies that they are independent of each other in early stage of recovery in mice.

关键词

Keywords

references

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