Resveratrol improves mitochondrial function <italic style="font-style: italic">via</italic> up-regulating Sirt3 in induced steatosis in HepG2 cells

Juan Huang; Xianglong Zeng; Qianyong Zhang; Mantian Mi

doi:10.16016/j.1000-5404.201609164

您当前的位置：

首页 >

文章列表页 >

Resveratrol improves mitochondrial function via up-regulating Sirt3 in induced steatosis in HepG2 cells

更新时间：2026-01-09

- Resveratrol improves mitochondrial function via up-regulating Sirt3 in induced steatosis in HepG2 cells
- Journal of Third Military Medical University Vol. 39, Issue 6, Pages: 536-540(2017)
- 作者机构：
  
  400038 重庆，第三军医大学军事预防医学院营养与食品安全研究中心，重庆市营养与食品安全重点实验室，重庆市医学营养研究中心
- 作者简介：
- 基金信息：
  
  the General Program of National Nature Science Foundation of China(81573131)
- DOI：10.16016/j.1000-5404.201609164
  CLC： R151.3;R322.47;R329.25
- Received：26 September 2016，
  
  Revised：2016-10-20，
  
  Published：2017-06
- 稿件说明：
移动端阅览
Juan Huang, Xianglong Zeng, Qianyong Zhang, et al. Resveratrol improves mitochondrial function via up-regulating Sirt3 in induced steatosis in HepG2 cells[J]. Journal of Third Military Medical University, 2017, 39(6): 536-540.
DOI：

Juan Huang, Xianglong Zeng, Qianyong Zhang, et al. Resveratrol improves mitochondrial function via up-regulating Sirt3 in induced steatosis in HepG2 cells[J]. Journal of Third Military Medical University, 2017, 39(6): 536-540. DOI： 10.16016/j.1000-5404.201609164.

摘要

目的

探讨白藜芦醇 (resveratrol，RSV) 对脂肪变性肝细胞线粒体功能的影响，以及沉默信息调节因子3(sirtuin 3，Sirt3) 在其中的重要作用。

方法

用0.2 mmol/L棕榈酸 (palmitic acid

PA) 处理HepG2细胞24 h建立肝细胞脂肪变性模型，再用40 μmol/L RSV和/或50 μmol/L Sirt3抑制剂3-TYP处理24 h。实验分为对照组、PA组、PA+RSV组、PA+RSV+3-TYP组、PA+3-TYP组。用相关试剂盒检测甘油三酯 (TG) 含量、沉默调节蛋白3(sirtuin 3，Sirt3) 活性、ATP合酶活性、ATP生成量以及线粒体基础呼吸率，使用DCFH-DA荧光探针检测细胞活性氧 (reactive oxygen species，ROS) 含量，Western blot检测Sirt3蛋白表达。Sirt3 siRNA处理HepG2细胞后再用PA和/或RSV处理，分析ROS和甘油三酯含量。

结果

相对于PA组，PA+RSV组HepG2细胞的甘油三酯、ROS含量明显降低 (

0.05)，ATP合酶活性、ATP生成量和基础呼吸率明显提高 (

0.05)。而3-TYP或Sirt3 siRNA处理后，RSV的上述作用被削弱 (

0.05)。

结论

RSV通过上调脂肪变性肝细胞Sirt3的表达与活性而改善其线粒体功能。

Abstract

Objective

To explore the influence of resveratrol (RSV) on mitochondrial function in induced steatosis in hepatic cells and investigate the role of sirtuin 3 (Sirt3) in it.

Methods

HepG2 cells were pretreated with palmitic acid (PA

0.2 mmol/L) for 24 h to establish hepatic steatosis cell model. Then

these hepatic steatosis cells were treated with 40 μmol/L RSV and/or 50 μmol/L 3-TYP (Sirt3 inhibitor) for 24 h. Then the cells were divided into 5 groups: control group

PA group

PA+RSV group

PA+RSV+3-TYP group

and PA+3-TYP group

respectively. The levels of triglycerides (TG) and ATP

Sirt3 activity

ATP synthase activity and basal oxygen consumption rate were measured using relevant kits. Reactive oxygen species (ROS) content was detected by DCFH-DA fluorescence probe. The expression of Sirt3 was measured by Western blotting.

Results

Compared with the PA group

the levels of TG and ROS in the PA+RSV group were decreased significantly (

0.05). On the contrary

the ATP synthase activity

ATP level and basal oxygen consumption rate were increased significantly (

0.05). However

the effects of RSV were impaired with 3-TYP or Sirt3 siRNA treatment (

0.05).

Conclusion

RSV can improve mitochondrial function by up-regulating the expression and activity of Sirt3 in steatosis in hepatic cells.

关键词

Keywords

references

Than N N, Newsome P N. A concise review of non-alcoholic fatty liver disease[J]. Atherosclerosis, 2015, 239(1): 192-202. DOI:10.1016/j.atherosclerosis.2015.01.001

Singer C, Stancu P, Cosoveanu S, et al. Non-alcoholic Fatty liver disease in children[J]. Curr Health Sci J, 2014, 40(3): 170-176. DOI:10.12865/CHSJ.40.03.03

Nassir F, Ibdah J A. Role of mitochondria in nonalcoholic fatty liver disease[J]. Int J Mol Sci, 2014, 15(5): 8713-8742. DOI:10.3390/ijms15058713

Heebøll S, Thomsen K L, Pedersen S B, et al. Effects of resveratrol in experimental and clinical non-alcoholic fatty liver disease[J]. World J Hepatol, 2014, 6(4): 188-198. DOI:10.4254/wjh.v6.i4.188

高燕翔, 张勇, 刘裕, 等.SREBP-1c在白藜芦醇预防大鼠非酒精性脂肪肝发生中的作用[J].第三军医大学学报, 2015, 37(17): 1704-1708. DOI: 10.16016/j.1000-5404.201412125

Gao Y X, Zhang Y, Liu Y, et al. SREBP-1c is involved in preventive effect of resveratrol on pathogenesis of nonalcoholic fatty liver disease in rats[J]. J Third Mil Med Univ, 2015, 37(17): 1704-1708. DOI: 10.16016/j.1000-5404.201412125

陈诗慧, 袁丽佳, 冉莉, 等.白藜芦醇对非酒精性脂肪性肝病患者血脂及血液流变学的影响[J].广州中医药大学学报, 2015, 32(6): 975-978.

Chen S h, Yuan L J, Ran L, et al. Clinical research of effect of resveratrol on serum lipids and hemorheology of patients with nonalcoholic fatty liver disease[J]. Journal of Guangzhou University of Traditional Chinese Medicine, 2015, 32(6): 975-978.

Zhang L, Han L, Ma R, et al. Sirt3 prevents maternal obesity-associated oxidative stress and meiotic defects in mouse oocytes[J]. Cell Cycle, 2015, 14(18): 2959-2968. DOI:10.1080/15384101.2015.1026517

Hirschey M D, Shimazu T, Goetzman E, et al. SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation[J]. Nature, 2010, 464(7285): 121-125. DOI:10.1038/nature08778

Koo S H. Nonalcoholic fatty liver disease: molecular mechanisms for the hepatic steatosis[J]. Clin Mol Hepatol, 2013, 19(3): 210-215. DOI:10.3350/cmh.2013.19.3.210

Takaki A, Kawai D, Yamamoto K. Multiple hits, including oxidative stress, as pathogenesis and treatment target in non-alcoholic steatohepatitis (NASH)[J]. Int J Mol Sci, 2013, 14(10): 20704-20728. DOI:10.3390/ijms141020704

Begriche K, Massart J, Robin M A, et al. Mitochondrial adaptations and dysfunctions in nonalcoholic fatty liver disease[J]. Hepatology, 2013, 58(4): 1497-1507. DOI:10.1002/hep.26226

Mojica-Villegas M A, Izquierdo-Vega J A, Chamorro-Cevallos G, et al. Protective effect of resveratrol on biomarkers of oxidative stress induced by iron/ascorbate in mouse spermatozoa[J]. Nutrients, 2014, 6(2): 489-503. DOI:10.3390/nu6020489

Mathieu L, Costa A L, Le Bachelier C, et al. Resveratrol attenuates oxidative stress in mitochondrial Complex Ⅰ deficiency: Involvement of SIRT3[J]. Free Radic Biol Med, 2016, 96: 190-198. DOI:10.1016/j.freeradbiomed.2016.04.027

Larson-Meyer D E, Newcomer B R, Heilbronn L K, et al. Effect of 6-month calorie restriction and exercise on serum and liver lipids and markers of liver function[J]. Obesity (Silver Spring), 2008, 16(6): 1355-1362. DOI:10.1038/oby.2008.201

Hirschey M D, Shimazu T, Jing E, et al. SIRT3 deficiency and mitochondrial protein hyperacetylation accelerate the development of the metabolic syndrome[J]. Mol Cell, 2011, 44(2): 177-190. DOI:10.1016/j.molcel.2011.07.019

Parihar P, Solanki I, Mansuri M L, et al. Mitochondrial sirtuins: emerging roles in metabolic regulations, energy homeostasis and diseases[J]. Exp Gerontol, 2015, 61: 130-141. DOI:10.1016/j.exger.2014.12.004

Haohao Z, Guijun Q, Juan Z, et al. Resveratrol improves high-fat diet induced insulin resistance by rebalancing subsarcolemmal mitochondrial oxidation and antioxidantion[J]. J Physiol Biochem, 2015, 71(1): 121-131. DOI:10.1007/s13105-015-0392-1

Chen T, Li J, Liu J, et al. Activation of SIRT3 by resveratrol ameliorates cardiac fibrosis and improves cardiac function via the TGF-β/Smad3 pathway[J]. Am J Physiol Heart Circ Physiol, 2015, 308(5): H424-H434. DOI:10.1152/ajpheart.00454.2014

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Resveratrol attenuates steatosis in cultured hepatic cells by inducing autophagy

Chronic hypoxia reduces cold tolerance via hepatic ANGPTL3-driven mitochondrial damage and adipose thermogenesis suppression

Role and mechanism of SIRT3 in oxidative stress and mitochondrial biogenesis in alveolar type II cells

Inhibitory effect and underlying mechanism of hepatocytes on bacterial proliferation

Protective effect of equol on mitochondrial damage induced by high glucose and fat in osteoblasts

Related Author

张勇

陈明亮

高燕翔

吴彬

吴瑛

张乾勇

糜漫天

HUANG Yujie

Related Institution

Department of Clinical Nursing, School of Nursing， Army Medical University （Third Military Medical University）

Key Laboratory of Extreme Environmental Medicine of Ministry of Education, School of Nursing， Army Medical University （Third Military Medical University）

Department of Frigid Zone Medicine， College of High Altitude Military Medicine, School of Nursing， Army Medical University （Third Military Medical University）

Institute of Field Surgery， Daping Hospital， Army Medical University

Department of Geriatrics and Special Service Medicine， First Affiliated Hospital（Southwest Hospital）， Army Medical University （Third Military Medical University）

⁰

Resveratrol improves mitochondrial function via up-regulating Sirt3 in induced steatosis in HepG2 cells

DOI：10.16016/j.1000-5404.201609164

摘要

Abstract

关键词

Keywords

references