Effect of periostin on proliferation of keloid mesenchymal stem cells <italic style="font-style: italic">in vitro</italic>

Xueli Wang; Xin Ma; Le Li; Xi Tan; Wenping Wang; Zhenxiang Wang

doi:10.16016/j.1000-5404.201609102

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Effect of periostin on proliferation of keloid mesenchymal stem cells in vitro

更新时间：2026-01-09

- Effect of periostin on proliferation of keloid mesenchymal stem cells in vitro
- Journal of Third Military Medical University Vol. 39, Issue 3, Pages: 236-242(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81272102)
- DOI：10.16016/j.1000-5404.201609102
  CLC：
- Received：14 September 2016，
  
  Published：15 February 2017
- 稿件说明：
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Xueli Wang, Xin Ma, Le Li, et al. Effect of periostin on proliferation of keloid mesenchymal stem cells in vitro[J]. Journal of Third Military Medical University, 2017, 39(3): 236-242.
DOI：

Xueli Wang, Xin Ma, Le Li, et al. Effect of periostin on proliferation of keloid mesenchymal stem cells in vitro[J]. Journal of Third Military Medical University, 2017, 39(3): 236-242. DOI： 10.16016/j.1000-5404.201609102.

摘要

Abstract

Objective

To determine the effect of periostin (also know as osteoblast-specific factor 2) on the proliferation of keloid mesenchymal stem cells (KMLSCs)

in vitro

Methods

KMLSCs and skin precursors (SKPs) were isolated from keloid tissues and the corresponding skin tissues respectively

and then cultured. The third passage cells were sorted by flow cytometry and then expanded. The expression of periostin was detected by immunocytochemical assay and Western blotting. The shPeriostin lentiviral vector was constructed

and then infected into KMLSCs. The interference efficiency was determined by qPCR and Western blotting. Cell proliferation

apoptosis and cell cycle in the KMLSCs after shPeriostin or blank vector infection and untreated cells were detected by MTT assay and flow cytometry. The expression of LRP5 was determined by immunofluorescent staining and Western blotting.

Results

The KMLSCs and SKPs were isolated from keloid and corresponding skin tissues

with

proportion of 94%. Immunocytochemical assay and Western blot analysis showed that the expression level of periostin was significantly higher in KMLSCs than in SKPs (

0.05). qPCR and Western blotting indicated that the expression of periostin was inhibited after the infection of shPeriostin lentiviral vector. Knockdown of periostin resulted in inhibitory proliferation

enhanced apoptosis and more cells arrested at G

phase in KMLSCs. Knockdown of periostin also led to the decreased expression of LRP5 (

0.05).

Conclusion

Knockdown of periostin induces apoptosis

inhibits cell cycle transition

and decreases proliferation in KMLSCs

which might be due to its effect on the expression of LRP5.

关键词

Keywords

references

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⁰

Effect of periostin on proliferation of keloid mesenchymal stem cells in vitro

DOI：10.16016/j.1000-5404.201609102

摘要

Abstract

关键词

Keywords

references