Peroxisome proliferators-activated receptor-gamma agonist inhibits angiotensin Ⅱ -induced phenotypic switching of vascular smooth muscle cells

Fei Gao; Xiaolu Hu; Kangning Chen

doi:10.16016/j.1000-5404.201609078

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Peroxisome proliferators-activated receptor-gamma agonist inhibits angiotensin Ⅱ -induced phenotypic switching of vascular smooth muscle cells

更新时间：2026-01-09

- Peroxisome proliferators-activated receptor-gamma agonist inhibits angiotensin Ⅱ -induced phenotypic switching of vascular smooth muscle cells
- Journal of Third Military Medical University Vol. 39, Issue 3, Pages: 229-235(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81271672)
- DOI：10.16016/j.1000-5404.201609078
  CLC：
- Received：11 September 2016，
  
  Published：15 February 2017
- 稿件说明：
移动端阅览
Fei Gao, Xiaolu Hu, Kangning Chen. Peroxisome proliferators-activated receptor-gamma agonist inhibits angiotensin Ⅱ -induced phenotypic switching of vascular smooth muscle cells[J]. Journal of Third Military Medical University, 2017, 39(3): 229-235.
DOI：

Fei Gao, Xiaolu Hu, Kangning Chen. Peroxisome proliferators-activated receptor-gamma agonist inhibits angiotensin Ⅱ -induced phenotypic switching of vascular smooth muscle cells[J]. Journal of Third Military Medical University, 2017, 39(3): 229-235. DOI： 10.16016/j.1000-5404.201609078.

摘要

Abstract

Objective

To investigate the inhibitory effect of peroxisome proliferator-activated receptor-gamma (PPAR-

) agonist

rosiglitazone (RSG)

on the phenotypic switching in vascular smooth muscle cells (VSMCs) induced by angiotensin Ⅱ (AngⅡ).

Methods

AngⅡ was employed to induce phenotypic switching in cultured mouse VSMCs. After pretreated with RSG and then treated with AngⅡ

the migration of VSMCs was analyzed by Transwell chamber assay

and F-actin polymerization was detected by Phalloidine-iFluor

488 staining under

laser confocal microscope. The protein and mRNA levels of PPAR-

PKGⅠα and smooth muscle 22 alpha (SM22α

marker molecule in differentiated VSMC with contractile phenotype) were measured by Western blotting and RT-qPCR. Also

Western blotting was employed to detect protein levels of PKGⅠα and SM22α in the VSMCs which were pretreated with PPAR-

inhibitor

GW9662 before RSG stimulation.

Results

RSG (20 μmol/L) pretreatment significantly inhibited AngⅡ-induced VSMCs immigration (

0.05) and formation of actin cytoskeleton. Results from RT-qPCR indicated that RSG pretreatment also reversed the down-regulation of PKGⅠα and SM22α at mRNA and protein levels in Ang Ⅱ-stimulated VSMCs (all

0.05). However

GW9662 treatment could attenuate the up-regulation of PKGⅠα and SM22α in RSG-treated VSMCs (

0.05).

Conclusion

RSG inhibits the phenotypic switching of VSMCs from

"contractile" to

"synthetic" state in response to Ang Ⅱ through activating PPAR-

and then up-regulating PKGⅠα.

关键词

Keywords

references

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