Effect of cyclophilin A downregulation on growth and metastasis of laryngeal squamous cell carcinoma in nude mice

您当前的位置：

首页 >

文章列表页 >

Effect of cyclophilin A downregulation on growth and metastasis of laryngeal squamous cell carcinoma in nude mice

更新时间：2026-01-09

- Effect of cyclophilin A downregulation on growth and metastasis of laryngeal squamous cell carcinoma in nude mice
- J Third Mil Med Univ Vol. 39, Issue 4, Pages: 355-360(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the National Clinical Key-discipline Construction Program of China(卫办医政函[2012]649号);the Key Project of Medical Scientific Research Chongqing Health Bureau(20121024)
- DOI：10.16016/j.1000-5404.201609064
  CLC：
- Received：08 September 2016，
  
  Published：28 February 2017
- 稿件说明：
移动端阅览
Lu Bai, Jianrong Zhou, Zhijuan Wang, et al. Effect of cyclophilin A downregulation on growth and metastasis of laryngeal squamous cell carcinoma in nude mice[J]. J Third Mil Med Univ, 2017, 39(4): 355-360.
DOI：

Lu Bai, Jianrong Zhou, Zhijuan Wang, et al. Effect of cyclophilin A downregulation on growth and metastasis of laryngeal squamous cell carcinoma in nude mice[J]. J Third Mil Med Univ, 2017, 39(4): 355-360. DOI： 10.16016/j.1000-5404.201609064.

摘要

Abstract

Objective

2

To explore the effect of RNA interference-mediated cyclophilin A (CypA) down-regulation on the growth and metastasis of laryngeal squamous cell carcinoma in nude mice.

Method

2

Lentiviral vector containing CypA shRNA was constructed and trans infected into Hep2 cells. Fifteen nude mice were randomly divided into 3 groups (

n

=5): control group

NC shRNA group

and CypA shRNA group. The growth and lymph node metastasis of tumors were observed. The expression of CypA

CD147 and matrix metalloproteinase (MMP)-9 in tumor tissues was detected by immunohistochemistry (IHC). The mRNA and protein expression levels of CypA

CD147 and MMP-9 were detected by real-time polymerase chain reaction (RT-PCR) assay and Western blotting

respectively.

Results

2

The gross tumor volume of CypA shRNA group was significantly smaller than those of both control and NC shRNA groups (

P

<

0.05). IHC staining showed cytoplasmic localization of CypA expression

whereas expression of CD147 and MMP-9 was detected in the cytomembrane and tumor stroma

respectively. The mRNA and protein expression levels of CypA

CD147 and MMP-9 were significantly decreased in CypA shRNA group as compared to control and NC shRNA groups (

P

<

0.05).

Conclusion

2

Down-regulation of cyclophilin A can inhibit the growth and metastasis of laryngeal squamous cell carcinoma by mediating the expression of CD147 and MMP-9.

关键词

Keywords

references

Szabó B Nelhubel G A Kárpáti A Clinical significance of genetic alterations and expression of epidermal growth factor receptor(EGFR) in head and neck squamous cell carcinomas Oral Oncol 2011 47 6 487 496 10.1016/j.oraloncology.2011.03.020 Szabó B, Nelhubel G A, Kárpáti A, et al. Clinical significance of genetic alterations and expression of epidermal growth factor receptor(EGFR) in head and neck squamous cell carcinomas[J]. Oral Oncol, 2011, 47(6): 487-496. DOI: 10.1016/j.oraloncology.2011.03.020

Qian Z Zhao X Jiang M Downregulation of cyclophilin A by siRNA diminishes non-small cell lung cancer cell growth and metastasis via the regulation of matrix metallopeptidase 9 BMC Cancer 2012 12 442 10.1186/1471-2407-12-442 Qian Z, Zhao X, Jiang M, et al. Downregulation of cyclophilin A by siRNA diminishes non-small cell lung cancer cell growth and metastasis via the regulation of matrix metallopeptidase 9[J]. BMC Cancer, 2012, 12: 442. DOI: 10.1186/1471-2407-12-442

Obchoei S Sawanyawisuth K Wongkham C Secreted cyclophilin A mediates G1/S phase transition of cholangiocarcinoma cells via CD147/ERK1/2 pathway Tumour Biol 2015 36 2 849 859 10.1007/s13277-014-2691-5 Obchoei S, Sawanyawisuth K, Wongkham C, et al. Secreted cyclophilin A mediates G1/S phase transition of cholangiocarcinoma cells via CD147/ERK1/2 pathway[J ] . Tumour Biol, 2015, 36(2): 849-859. DOI: 10.1007/s13277-014-2691-5

Zhu D Wang Z Zhao J J The Cyclophilin A CD147 complex promotes the proliferation and homing of multiple myeloma cells Nat Med 2015 21 6 572 580 10.1038/nm.3867 Zhu D, Wang Z, Zhao J J, et al. The Cyclophilin A CD147 complex promotes the proliferation and homing of multiple myeloma cells[J]. Nat Med, 2015, 21(6): 572-580 .DOI:10.1038/nm.3867

Han W Soltani K Ming M Deregulation of XPC and CypA by cyclosporin A: an immunosuppression-independent mechanism of skin carcinogenesis Cancer Prev Res (Phila) 2012 5 9 1155 1162 10.1158/1940-6207.CAPR-12-0185-T Han W, Soltani K, Ming M, et al. Deregulation of XPC and CypA by cyclosporin A: an immunosuppression-independent mechanism of skin carcinogenesis[J]. Cancer Prev Res (Phila), 2012, 5(9): 1155-1162. DOI: 10.1158/1940-6207.CAPR-12-0185-T

Li Z Min W Gou J Knockdown of cyclophilin A reverses paclitaxel resistance in human endometrial cancer cells via suppression of MAPK kinase pathways Cancer Chemother Pharmacol 2013 72 5 1001 1011 10.1007/s00280-013-2285-8 Li Z, Min W, Gou J. Knockdown of cyclophilin A reverses paclitaxel resistance in human endometrial cancer cells via suppression of MAPK kinase pathways[J]. Cancer Chemother Pharmacol, 2013, 72(5): 1001-1011. DOI: 10.1007/s00280-013-2285-8

León X Bothe C García J Expression of IL-1ɑ correlates with distant metastasis in patients with head and neck Squamous cell carcinoma Oncotarget 2015 6 35 37398 37409 10.18632/oncotarget.6054 León X, Bothe C, García J, et al. Expression of IL-1ɑ correlates with distant metastasis in patients with head and neck Squamous cell carcinoma[J]. Oncotarget, 2015, 6(35): 37398-37409. DOI: 10.18632/oncotarget.6054

Zhang S Y Lu Z M Luo X N Retrospective analysis of prognostic factors in 205 patients with Laryngeal squamous cell carcinoma who underwent surgical treatment PLoS One 2013 8 4 e60157 10.1371/journal.pone.0060157 Zhang S Y, Lu Z M, Luo X N, et al. Retrospective analysis of prognostic factors in 205 patients with Laryngeal squamous cell carcinoma who underwent surgical treatment[J]. PLoS One, 2013,8(4):e60157. DOI: 10.1371/journal.pone.0060157

Cho S Kang S G Tae B S Influence of nonregional lymph node metastasis as a prognostic factor in metastatic prostate Cancer patients Korean J Urol 2012 53 10 673 679 10.4111/kju.2012.53.10.673 Cho S, Kang S G, Tae B S, et al. Influence of nonregional lymph node metastasis as a prognostic factor in metastatic prostate Cancer patients[J]. Korean J Urol, 2012,53(10): 673-679. DOI: 10.4111/kju.2012.53.10.673

Dawer F U Tu J Khattak M N CyclophilinA: A key Factor in Virus Replication and Potential Target for Anti-viral Therapy Curr lssues Mol Biol 2016 21 1 20 Dawer F U, Tu J, Khattak M N, et al.CyclophilinA: A key Factor in Virus Replication and Potential Target for Anti-viral Therapy[J]. Curr lssues Mol Biol, 2016, 21: 1-20.

Saleh T Jarkowski W Sriram G CyclophilinA promotes cell migration via the Abl-Crk signaling pathway Nat Chem Biol 2016 12 2 117 123 10.1038/nchembio.1981 Saleh T, Jarkowski W, Sriram G, et al. CyclophilinA promotes cell migration via the Abl-Crk signaling pathway[J]. Nat Chem Biol, 2016, 12(2): 117-123. DOI: 10.1038/nchembio.1981

Nigro P Pompilio G Capogrossi M C Cyclophilin A: a key player for human disease Cell Death Dis 2013 4 e888 10.1038/cddis.2013.410 Nigro P, Pompilio G, Capogrossi M C. Cyclophilin A: a key player for human disease[J]. Cell Death Dis, 2013,4: e888. DOI: 10.1038/cddis.2013.410

冯明亮周建荣魏莲枝 MRP8和亲环素A在喉鳞状细胞癌组织中的表达及其临床意义临床耳鼻咽喉头颈外科杂志 2011 25 18 834 836 http://www.cnki.com.cn/Article/CJFDTOTAL-LCEH201118007.htm 冯明亮, 周建荣, 魏莲枝, 等. MRP8和亲环素A在喉鳞状细胞癌组织中的表达及其临床意义[J]. 临床耳鼻咽喉头颈外科杂志, 2011, 25(18): 834-836,839.

Dean N Helman E Aldridge J Anti-EMMPRIN treatment of HNSCC in an ex vivo model Laryngoscope 2010 120 Suppl 4 S146 10.1002/lary.21610 Dean N, Helman E, Aldridge J, et al. Anti-EMMPRIN treatment of HNSCC in an ex vivo model[J ] .Laryngoscope, 2010, 120( Suppl 4): S146. DOI: 10.1002/lary.21610

Dean N R Knowles J A Helman E E Anti-EMMPRIN antibody treatment of head and neck squamous cell carcinoma in an ex-vivo model Anticancer Drugs 2010 21 9 861 867 10.1097/CAD.0b013e32833d1a11 Dean N R, Knowles J A, Helman E E, et al. Anti-EMMPRIN antibody treatment of head and neck squamous cell carcinoma in an ex-vivo model[J ] . Anticancer Drugs, 2010, 21(9): 861-867. DOI:10.1097/CAD.0b013e328 33d1a11

Walter M Simanovich E Brod V An eiptope-specific novel anti-EMMPRIN polyclonal antibody inhibits tumor progression Oncoimmunology 2015 5 2 e1078056 10.1080/2162402X.2015.1078056 Walter M, Simanovich E, Brod V, et al. An eiptope-specific novel anti-EMMPRIN polyclonal antibody inhibits tumor progression[J]. Oncoimmunology, 2015, 5(2): e1078056. DOI: 10.1080/2162402X.2015.1078056

Kumari S Roy S Singh P Cyclophilins: proteins in search of function Plant signal Behav 2013 8 1 e22734 10.4161/psb.22734 Kumari S, Roy S, Singh P, et al. Cyclophilins: proteins in search of function[J]. Plant signal Behav, 2013, 8(1): e22734. DOI: 10.4161/psb.22734

Bahmed K Henry C Holliday M Extracellular Cyclophilin-A stimulates ERK1/2 phosphorylation in a cell-dependent manner but broadly stimulates nuclear factor Kappa-B Cancer cell Int 2012 12 19 10.1186/1475-2867-12-19 Bahmed K, Henry C, Holliday M, et al. Extracellular Cyclophilin-A stimulates ERK1/2 phosphorylation in a cell-dependent manner but broadly stimulates nuclear factor Kappa-B[J]. Cancer cell Int, 2012, 12: 19. DOI: 10.1186/1475-2867-12-19

Frederick J W Sweeny L Hartman Y Epidermal growth factor receptor inhibition by anti-CD147 therapy in cutaneous squamous cell carcinoma Head Neck 2016 38 2 247 252 10.1002/hed.23885 Frederick J W, Sweeny L, Hartman Y, et al. Epidermal growth factor receptor inhibition by anti-CD147 therapy in cutaneous squamous cell carcinoma[J]. Head Neck, 2016, 38(2): 247-252. DOI: 10.1002/hed.23885

0

Views

0

下载量

0

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Related Articles

Construction and identification of lentiviral vector for RNA interference of human Versican gene and role of Versican in aggregative growth of dermal papilla cells

Silencing nucleostemin in EC9706 cells inhibits tumor growth and expression of EGF and EGFR in xenograft tumor in nude mice

RNAi-mediated silencing of HIF-1α inhibits growth and metastasis of colon cancer in tumor-bearing mice

Karyopherina 2 knockdown inhibits osteosarcoma cell proliferation and angiogenesis in mice by suppressing Sox2 expression

Effect of miR-221 on growth of transplantation tumor of neuroblastoma cell line SH-SY5Y in nude mice

Related Author

封明霞

伍津津

杨桂红

张功员

关文池

李晟磊

张巧

吴卫东

Related Institution

Department of Orthopedics, Second Hospital of Shanxi Medical University

Center for Clinical Molecular Medicine, Key Laboratory of Child Development and Disorders of Ministry of Education, Chongqing Key Laboratory of Pediatrics, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Children's Hospital of Chongqing Medical University

Department of Pharmacognosy, Faculty of Pharmacy & Laboratory Medicine, Army Medical University (Third Military Medical University)

Department of Hematology, the First Affiliated Hospital of Chongqing Medical University

Department of Scientific Research Affairs, the Second Affiliated Hospital of Chongqing Medical University

⁰