Recording of discharge of mouse jugular vagus and its application during renal ischemia-reperfusion

Jianbo Ma; Juanjuan Li; Kaizhi Lu; Jianteng Gu

doi:10.16016/j.1000-5404.201608179

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Recording of discharge of mouse jugular vagus and its application during renal ischemia-reperfusion

更新时间：2026-01-09

- Recording of discharge of mouse jugular vagus and its application during renal ischemia-reperfusion
- Journal of Third Military Medical University Vol. 39, Issue 3, Pages: 249-252(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81272068)
- DOI：10.16016/j.1000-5404.201608179
  CLC：
- Received：28 September 2016，
  
  Published：15 February 2017
- 稿件说明：
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Jianbo Ma, Juanjuan Li, Kaizhi Lu, et al. Recording of discharge of mouse jugular vagus and its application during renal ischemia-reperfusion[J]. Journal of Third Military Medical University, 2017, 39(3): 249-252.
DOI：

Jianbo Ma, Juanjuan Li, Kaizhi Lu, et al. Recording of discharge of mouse jugular vagus and its application during renal ischemia-reperfusion[J]. Journal of Third Military Medical University, 2017, 39(3): 249-252. DOI： 10.16016/j.1000-5404.201608179.

摘要

Abstract

Objective

To record the frequency variations of jugular vagus discharge in mouse model of renal ischemia-reperfusion

and introduce the method and experimental skills of the recording.

Methods

The right cervical vagal nerve of 8 male C57BL/6J mice (8~10 weeks old

weighing 22~26 g) was exposed and then bluntly separated through

midline cervical incision. The vagus nerve discharge was conducted by

pair of bipolar stainless steel electrodes and then recorded. Renal ischemia was induced by bilateral renal pedicle clamping for 25 min and followed by reperfusion for 30 min. The frequency variations of vagus nerve discharge were recorded by the electrodes during the whole process.

Results

Compared with the basic values

the discharge frequency were increased from 520±52 Hz to 632±59 Hz (

0.05) in ischemic phase

and furthermore it kept in an increasing trend during 15 minutes' reperfusion and then reached the peak (672±58 Hz

0.05)

but then was gradually decreased to 544±51 Hz after reperfusion (

0.05).

Conclusion

The vagus discharge is significantly increased during renal ischemia-reperfusion

which might be due to neural protection in the process.

关键词

Keywords

references

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