DcR2 expression in renal tissue and urine after ischemia reperfusion related to kidney injury in rats

Jiarui Liu; Jia Chen; Kehong Chen; Lirong Lin; Jianguo Zhang; Kailong Li; Luquan Zheng; Yani He

doi:10.16016/j.1000-5404.201608163

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DcR2 expression in renal tissue and urine after ischemia reperfusion related to kidney injury in rats

更新时间：2026-01-09

- DcR2 expression in renal tissue and urine after ischemia reperfusion related to kidney injury in rats
- J Third Mil Med Univ Vol. 39, Issue 4, Pages: 342-348(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(8164792)
- DOI：10.16016/j.1000-5404.201608163
  CLC：
- Received：30 August 2016，
  
  Published：28 February 2017
- 稿件说明：
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Jiarui Liu, Jia Chen, Kehong Chen, et al. DcR2 expression in renal tissue and urine after ischemia reperfusion related to kidney injury in rats[J]. J Third Mil Med Univ, 2017, 39(4): 342-348.
DOI：

Jiarui Liu, Jia Chen, Kehong Chen, et al. DcR2 expression in renal tissue and urine after ischemia reperfusion related to kidney injury in rats[J]. J Third Mil Med Univ, 2017, 39(4): 342-348. DOI： 10.16016/j.1000-5404.201608163.

摘要

Abstract

Objective

To observe the expression of decoy receptor 2 (DcR2) in renal tissue and urine of rat after ischemia reperfusion injury (I/R) for investigating its correlation to kidney injury.

Method

Forty healthy male SD rats were randomized into sham operation group and I/R 60 min group. Blood

urine and bilateral renal tissues were collected at days 1

21 and 35 after operation. The levels of serum creatinine (Scr) and urinary NAG (uNAG) were detected by biochemical analysis. Renal morphologic changes were observed and pathologically assessed by PAS and Masson's trichrome staining. The expression of DcR2 in renal tissues was detected by immunohistochemical assay

and the expression of urine DcR2 (uDcR2) was measured by enzyme-linked immunosorbent assay (ELISA).

Results

Compared to the Scr and uNAG levels in the sham group (21.3±1.6 and 4.4±0.6 U/mmol

respectively)

they significantly increased in the I/R 60 min group(

0.05). At days 1

21 and 35 post operation

the Scr levels were 122.2±15.4

193.5±27.7

66.4±10.2

37.8±6.2

and 34.4±2.8 μmol/L

respectively. Accordingly

the uNAG levels were 9.8±2.0

17.0±2.5

7.8±1.5

6.5±2.1

6.7±2.3 U/mmol

respectively. Obvious pathological changes were found at the corticomedullary junction in the I/R 60 min group

including acute tubular necrosis

denudation

brush border and formation of cellular casts

which progressed to renal tubule atrophy

interstitial fibrosis and chronic kidney disease in later stage of reperfusion. Additionally

the expression of DcR2 in renal tissue and urine was increased significantly in the I/R 60 min group compared to the sham group(

0.05). The increased level of DcR2 and uDcR2 were positively correlated with renal function and kidney injury score (

0.05). Meanwhile

the Masson staining showed that in the I/R 60 min group renal DcR2 expression was positively correlated with interstitial fibrosis (

0.05).

Conclusion

The expression of DcR2 in renal tissue and uDcR2 level are positively related to renal function and kidney injury score in rats with I/R injury. This may suggest the important role of DcR2 played in the progress of renal interstitial fibrosis after ischemia reperfusion.

关键词

Keywords

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