Effect of lncRNA-Gm4419 on proliferation and fibrosis in high glucose-induced glomerulus mesangial cells

Hong Yi; Rui Peng; Yan Sun; Yongjun Luo; Huimin Peng; Ailing Li; Zheng Zhang

doi:10.16016/j.1000-5404.201608102

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Effect of lncRNA-Gm4419 on proliferation and fibrosis in high glucose-induced glomerulus mesangial cells

更新时间：2026-01-09

- Effect of lncRNA-Gm4419 on proliferation and fibrosis in high glucose-induced glomerulus mesangial cells
- Journal of Third Military Medical University Vol. 39, Issue 3, Pages: 216-222(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Advanced Research Program of National Natural Science Foundation of China(NSFYY201524);the Nursery Foundation of College of Basic Medical Sciences of Chongqing Medical University in 2015(JC201508)
- DOI：10.16016/j.1000-5404.201608102
  CLC：
- Received：19 August 2016，
  
  Published：15 February 2017
- 稿件说明：
移动端阅览
Hong Yi, Rui Peng, Yan Sun, et al. Effect of lncRNA-Gm4419 on proliferation and fibrosis in high glucose-induced glomerulus mesangial cells[J]. Journal of Third Military Medical University, 2017, 39(3): 216-222.
DOI：

Hong Yi, Rui Peng, Yan Sun, et al. Effect of lncRNA-Gm4419 on proliferation and fibrosis in high glucose-induced glomerulus mesangial cells[J]. Journal of Third Military Medical University, 2017, 39(3): 216-222. DOI： 10.16016/j.1000-5404.201608102.

摘要

Abstract

Objective

To determine the effect of long non-coding RNA (lncRNA) Gm4419 on the proliferation and fibrosis of mouse glomerulus mesangial cells under high glucose condition.

Methods

The expression of Gm4419 in the kidney tissues from the mouse model of diabetic nephropathy (DN) and glomerulus mesangial cells cultured under high glucose condition were detected by fluorescence qRT-PCR. Gm4419 siRNAs were designed and synthesized

and over-expression plasmid of pcDNA3.1 (+)-Gm4419 was constructed. Then the Gm4419 siRNAs and pcDNA3.1 (+)-Gm4419 over-expression plasmids were transiently transfected respectively into the glomerulus mesangial cells under high or low glucose condition. EdU assay was used to detect the proliferation ability of the transfected cells. Western blotting was employed to detect the protein levels of renal fibrosis markers.

Results

The expression levels of Gm4419 were significantly higher in the kidney tissues of DN mice and high-glucose cultured glomerulus mesangial cells when compared with the tissues of control mice and low-glucose cultured cells (

0.01). After the Gm4419 siRNA with best interfering effect screened by fluorescence qRT-PCR was transfected into the high-glucose cells

the knockdown resulted in the decrease in the proliferation and fibrosis in the cells when compared with high glucose mock cells or control cells (

0.05). In addition

after the over-expression plasmid pcDNA3.1 (+)-Gm4419 confirmed by enzyme digestion and DNA sequencing was transfected into the cells cultured under low glucose condition

the proliferation ability and fibrosis were enhanced compared with low glucose mock cells or control group (

0.05).

Conclusion

lncRNA-Gm4419 is involved in the regulation of the proliferation and fibrosis of mesangial cells from DN mice.

关键词

Keywords

references

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