Expression of <italic style="font-style: italic">rap1b</italic> in <italic style="font-style: italic">hoxb4</italic> transgenic zebrafish during embryo hematopoietic development

Ranran Liu; Xiaoyan Yang; Muyuan Ji; Xijun Wu; Anran Fan; Liping Shu; Zhixu He

doi:10.16016/j.1000-5404.201608093

您当前的位置：

首页 >

文章列表页 >

Expression of rap1b in hoxb4 transgenic zebrafish during embryo hematopoietic development

更新时间：2026-01-09

- Expression of rap1b in hoxb4 transgenic zebrafish during embryo hematopoietic development
- Journal of Third Military Medical University Vol. 39, Issue 3, Pages: 243-248(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(31360285)
- DOI：10.16016/j.1000-5404.201608093
  CLC：
- Received：19 August 2016，
  
  Published：15 February 2017
- 稿件说明：
移动端阅览
Ranran Liu, Xiaoyan Yang, Muyuan Ji, et al. Expression of rap1b in hoxb4 transgenic zebrafish during embryo hematopoietic development[J]. Journal of Third Military Medical University, 2017, 39(3): 243-248.
DOI：

Ranran Liu, Xiaoyan Yang, Muyuan Ji, et al. Expression of rap1b in hoxb4 transgenic zebrafish during embryo hematopoietic development[J]. Journal of Third Military Medical University, 2017, 39(3): 243-248. DOI： 10.16016/j.1000-5404.201608093.

摘要

Abstract

Objective

To investigate the expression pattern of

rap1b

gene in the zebrafish with

hoxb4

transgene and the relationship between the 2 genes in early hematopoietic development so as to find critical regulatory factors in the early hematopoietic development.

Methods

Zebrafish with highly expressed

hoxb4

were selected as the research object. There were 3 groups of zebrafish

namely the exprimental group (zebrafish expressed

hoxb4

EGFP

)

the control group (zebrafish only expressed

EGFP

) and the blank control group (wild type zebrafish). Embryo cells with

EGFP

fluorescence signals were sorted on 18 hours post fertilization (hpf)

and 24

36 and 48 hpf respectively from control groups and experimental group. The expression of

rap1b

was examined with quantitative real-time PCR (qRT-PCR). Then

the multi-phase embryos of wild type zebrafish were collected to prepare

rap1b

gene antisense mRNA probes

and the hybridization signals of

rap1b

in the 3 gro

ups of zebrafish embryos were observed on 18

36 and 48 hpf with whole-mount

in situ

hybridization (WISH).

Results

The results of qRT-PCR showed that the expression of

rap1b

gene was significantly enhanced on 30

36 and 48 hpf in the experimental group (

0.05). Antisense mRNA probes of

rap1b

gene were successfully achieved

and WISH results showed that

rap1b

gene was expressed in the range of 18 to 48 hpf in the nervous system and the hematologic system in 3 groups of zebrafish embryos.

Conclusion

The expression of

rap1b

gene is obviously increased in

hoxb4

transgenic zebrafish

suggesting that

rap1b

and

hoxb4

may be both involved in the regulation of early hematopoietic development.

关键词

Keywords

references

Yasunaga S Ohno Y Shirasu N Role of Geminin in cell fate determination of hematopoietic stem cells (HSCs) Int J Hematol 2016 104 3 324 329 10.1007/s12185-016-2060-9 Yasunaga S, Ohno Y, Shirasu N, et al . Role of Geminin in cell fate determination of hematopoietic stem cells (HSCs)[J ] . Int J Hematol, 2016, 104(3): 324-329.DOI: 10.1007/s12185-016-2060-9

Jackson M Ma R Taylor A H Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells Stem Cells Transl Med 2016 5 8 981 990 10.5966/sctm.2015-0324 Jackson M, Ma R, Taylor A H, et al . Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells[J ] . Stem Cells Transl Med, 2016, 5(8): 981-990.DOI: 10.5966/sctm.2015-0324

Xin C Zhao C Yin X Bioinformatics analysis of molecular mechanism of the expansion of hematopoietic stem cell transduced by HOXB4/HOXC4 Hematology 2016 21 8 462 469 10.1080/10245332.2015.1101978 Xin C, Zhao C, Yin X, et al . Bioinformatics analysis of molecular mechanism of the expansion of hematopoietic stem cell transduced by HOXB4/HOXC4[J ] . Hematology, 2016, 21(8): 462-469.DOI: 10.1080/10245332.2015.1101978

Morsi El-Kadi A S in der Reiden P Durston A The small GTPase Rap1 is an immediate downstream targetfor Hoxb4 transcriptional regulation Mech Dev 2002 113 2 131 139 10.1016/S0925-4773(02)00047-3 Morsi El-Kadi A S, in der Reiden P, Durston A, et al . The small GTPase Rap1 is an immediate downstream targetfor Hoxb4 transcriptional regulation[J ] . Mech Dev, 2002, 113(2 ): 131-139.DOI: 10.1016/S0925-4773(02)00047-3

Shu L P Zhou Z W Zhou T Ectopic expression of Hoxb4a in hemangioblasts promotes hematopoietic development in early embryogenesis of zebrafish Clin Exp Pharmacol Physiol 2015 42 12 1275 1286 10.1111/1440-1681.12483 Shu L P, Zhou Z W, Zhou T, et al . Ectopic expression of Hoxb4a in hemangioblasts promotes hematopoietic development in early embryogenesis of zebrafish[J ] . Clin Exp Pharmacol Physiol, 2015, 42(12): 1275-1286.DOI: 10.1111/1440-1681.12483

He J H Gao J M Huang C J Zebrafish models for assessing developmental and reproductive toxicity Neurotoxicol Teratol 2014 42 35 42 10.1016/j.ntt.2014.01.006 He J H, Gao J M, Huang C J, et al . Zebrafish models for assessing developmental and reproductive toxicity[J ] . Neurotoxicol Teratol, 2014, 42: 35-42.DOI: 10.1016/j.ntt.2014.01.006

Zhang C Patient R Liu F Hematopoietic stem cell development and regulatory signaling in zebrafish Biochim Biophys Acta 2013 1830 2 2370 2374 10.1016/j.bbagen.2012.06.008 Zhang C, Patient R, Liu F. Hematopoietic stem cell development and regulatory signaling in zebrafish[J]. Biochim Biophys Acta, 2013, 1830(2): 2370-2374.DOI: 10.1016/j.bbagen.2012.06.008

Rastegar S Strahle U The Zebrafish as Model for Deciphering the Regulatory Architecture of Vertebrate Genomes Adv Genet 2016 95 195 216 10.1016/bs.adgen.2016.04.003 Rastegar S, Strahle U. The Zebrafish as Model for Deciphering the Regulatory Architecture of Vertebrate Genomes[J]. Adv Genet, 2016, 95: 195-216.DOI: 10.1016/bs.adgen.2016.04.003

Dong W Yang Z Yang F Suppression of Rap1 impairs cardiac myofibrils and conduction system in zebrafish PLoS One 2012 7 11 e50960 10.1371/journal.pone.0050960 Dong W, Yang Z, Yang F, et al . Suppression of Rap1 impairs cardiac myofibrils and conduction system in zebrafish[J ] . PLoS One, 2012, 7(11): e50960.DOI: 10.1371/journal.pone.0050960

Azoulay-Alfaguter I Strazza M Mor A Chaperone-mediated specificity in Ras and Rap signaling Crit Rev Biochem Mol Biol 2015 50 3 194 202 10.3109/10409238.2014.989308 Azoulay-Alfaguter I, Strazza M, Mor A. Chaperone-mediated specificity in Ras and Rap signaling[J]. Crit Rev Biochem Mol Biol, 2015, 50(3): 194-202.DOI: 10.3109/10409238.2014.989308

Zhang H Chang Y C Brennan M L The structure of Rap1 in complex with RIAM reveals specificity determinants and recruitment mechanism J Mol Cell Biol 2014 6 2 128 139 10.1093/jmcb/mjt044 Zhang H, Chang Y C, Brennan M L, et al . The structure of Rap1 in complex with RIAM reveals specificity determinants and recruitment mechanism[J ] . J Mol Cell Biol, 2014, 6(2): 128-139.DOI: 10.1093/jmcb/mjt044

Kumar S Xu J Kumar R S The small GTPase Rap1b negatively regulates neutrophil chemotaxis and transcellular diapedesis by inhibiting Akt activation J Exp Med 2014 211 9 1741 1758 10.1084/jem.20131706 Kumar S, Xu J, Kumar R S, et al . The small GTPase Rap1b negatively regulates neutrophil chemotaxis and transcellular diapedesis by inhibiting Akt activation[J ] . J Exp Med, 2014, 211(9): 1741-1758.DOI: 10.1084/jem.20131706

Chrzanowska-Wodnicka M White G C 2nd Quilliam L A Small GTPase Rap1 Is Essential for Mouse Development and Formation of Functional Vasculature PLoS One 2015 10 12 e0145689 10.1371/journal.pone.0145689 Chrzanowska-Wodnicka M, White G C 2nd, Quilliam L A, et al . Small GTPase Rap1 Is Essential for Mouse Development and Formation of Functional Vasculature[J ] . PLoS One, 2015, 10(12): e0145689.DOI: 10.1371/journal.pone.0145689

Szklanna P B Foy M Wynne K Analysis of the proteins associated with platelet detergent resistant membranes Proteomics 2016 16 17 2345 2350 10.1002/pmic.201500309 Szklanna P B, Foy M, Wynne K, et al . Analysis of the proteins associated with platelet detergent resistant membranes[J ] . Proteomics, 2016, 16(17): 2345-2350.DOI: 10.1002/pmic.201500309

Lakshmikanthan S Sobczak M Chun C Rap1 promotes VEGFR2 activation and angiogenesis by a mechanism involving integrin αvβ3 Blood 2011 118 7 2015 2026 10.1182/blood-2011-04-349282 Lakshmikanthan S, Sobczak M, Chun C, et al . Rap1 promotes VEGFR2 activation and angiogenesis by a mechanism involving integrin αvβ3[J ] . Blood, 2011, 118(7): 2015-2026.DOI: 10.1182/blood-2011-04-349282

Lakshmikanthan S Zieba B J Ge Z D Rap1b in smooth muscle and endothelium is required for maintenance of vascular tone and normal blood pressure Arterioscler Thromb Vasc Biol 2014 34 7 1486 1494 10.1161/ATVBAHA.114.303678 Lakshmikanthan S, Zieba B J, Ge Z D, et al . Rap1b in smooth muscle and endothelium is required for maintenance of vascular tone and normal blood pressure[J ] . Arterioscler Thromb Vasc Biol, 2014, 34(7): 1486-1494.DOI: 10.1161/ATVBAHA.114.303678

Gore A V Lampugnani M G Dye L Combinatorial interaction between CCM pathway genes precipitates hemorrhagic stroke Dis Model Mech 2008 1 4/5 275 281 10.1242/dmm.000513 Gore A V, Lampugnani M G, Dye L, et al . Combinatorial interaction between CCM pathway genes precipitates hemorrhagic stroke[J ] . Dis Model Mech, 2008, 1(4/5): 275-281.DOI: 10.1242/dmm.000513

Chrzanowska-Wodnicka M Kraus A E Gale D Defective angiogenesis, endothelial migration, proliferation, and MAPK signaling in Rap1b-deficient mice Blood 2008 111 5 2647 2656 10.1182/blood-2007-08109710 Chrzanowska-Wodnicka M, Kraus A E, Gale D, et al . Defective angiogenesis, endothelial migration, proliferation, and MAPK signaling in Rap1b-deficient mice[J ] . Blood, 2008, 111(5): 2647-2656.DOI: 10.1182/blood-2007-08109710

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Molecular mechanism of Zic3 controlling embryonic cerebrovascular development via non-classical Wnt and VEGF signaling axes in zebrafish

Effects of different concentrations of dimethylsulfoxide on development of zebrafish embryos and dopamine neurons

Developmental toxicity of embryonic 1-naphthol exposure in zebrafish

A zebrafish model of Parkinson's disease established using mycalolide B

Related Author

Liping SHU

Hua LI

Yanhua ZHOU

Yichen YANG

Tao PENG

Dongzhen ZHOU

Weijun ZHOU

Huizhe HUANG

Related Institution

Department of Developmental Biology, Reproductive Medicine Center, the Second Affiliated Hospital of Chongqing Medical University

College of Biological Engineering, Chongqing University

Department of Anatomy, Histology and Embryology, Sichuan Provincial Key Laboratory of Development and Regeneration, Chengdu Medical College

Department of Pediatrics, Guizhou Medical University

Tissue Engineering and Stem Cell Center, Guizhou Medical University, Key Laboratory of Adult Stem Cell Transformation Research of Chinese Academy of Medical Sciences

⁰

Expression of rap1b in hoxb4 transgenic zebrafish during embryo hematopoietic development

DOI：10.16016/j.1000-5404.201608093

摘要

Abstract

关键词

Keywords

references