Establishment of a human primary dendritic cell model (CD209,-139A/-336A)infected with dengue virus

Yi Yang; Xin Fang; Zhen Hu; Jie Yang; Jizhen Yuan; Weilong Shang; Xiancai Rao

doi:10.16016/j.1000-5404.201607134

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Establishment of a human primary dendritic cell model (CD209,-139A/-336A)infected with dengue virus

更新时间：2026-01-09

- Establishment of a human primary dendritic cell model (CD209,-139A/-336A)infected with dengue virus
- J Third Mil Med Univ Vol. 39, Issue 4, Pages: 323-327(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Major Project of National Science and Technology(2012ZX09103301-038);the National Natural Science Foundation for Young Scholars of China(31200133)
- DOI：10.16016/j.1000-5404.201607134
  CLC：
- Received：20 July 2016，
  
  Published：28 February 2017
- 稿件说明：
移动端阅览
Yi Yang, Xin Fang, Zhen Hu, et al. Establishment of a human primary dendritic cell model (CD209,-139A/-336A)infected with dengue virus[J]. J Third Mil Med Univ, 2017, 39(4): 323-327.
DOI：

Yi Yang, Xin Fang, Zhen Hu, et al. Establishment of a human primary dendritic cell model (CD209,-139A/-336A)infected with dengue virus[J]. J Third Mil Med Univ, 2017, 39(4): 323-327. DOI： 10.16016/j.1000-5404.201607134.

摘要

Abstract

Objective

To establish a human primary dendritic cells(DCs) model infected with dengue virus (DENV).

Method

Peripheral blood samples defined with DC genotype of CD209

-139A/-336A were collected. The DC precursor cells were isolated by ficoll-gastrografin density gradient centrifugation and further induced to differentiate into immature DCs by culturing in RPMI1640 media supplemented with recombinant human granulocyte macrophage-colony stimulating factor (rhGM-CSF) and interleukin-4 (rhIL-4). The differentiation efficiency was determined by flow cytometry.The morphologic and biological characteristics of DCs were identified by Wright’s staining and lymphocyte proliferation assay

respectively. Finally

DENV-1 strain GZ2002 was used for construction and evaluation of the DENV infection in human DCs.

Results

The immature DCs with typical morphologic characteristics were successfully obtained from the isolated precursor cells with cytokine stimulation

of which the differentiation efficiency was greater than 90%. The acquired DCs could effectively stimulate lymphocyte proliferation and support the entire viral lifecycle during DENV infection

suggesting the successful establishment of DENV infected DC model.

Conclusion

DENV infection model based on human primary DCs is successfully established.

关键词

Keywords

references

Bhatt S Gething P W Brady O J The global distribution and burden of dengue Nature 2013 496 7446 504 507 10.1038/nature12060 Bhatt S, Gething P W, Brady O J, et al. The global distribution and burden of dengue[J]. Nature, 2013, 496(7446): 504-507. DOI: 10.1038/ nature12060

Wang L Yang G Jia L Epidemiologic characteristics of dengue in China (2010-2014) J Infect 2015 71 3 397 399 10.1016/j.jinf.2015.04.018 Wang L, Yang G, Jia L, et al. Epidemiologic characteristics of dengue in China (2010-2014)[J]. J Infect, 2015, 71(3): 397-399. DOI: 10.1016/j.jinf.2015.04.018

Wu S J Grouard-Vogel G Sun W Human skin Langerhans cells are targets of dengue virus infection Nat Med 2000 6 7 816 820 10.1038/77553 Wu S J, Grouard-Vogel G, Sun W, et al. Human skin Langerhans cells are targets of dengue virus infection[J]. Nat Med, 2000, 6(7): 816-820. DOI: 10.1038/77553

Sakuntabhai A Turbpaiboon C Casadémont I A variant in the CD209 promoter is associated with severity of dengue disease Nat Genet 2005 37 5 507 513 10.1038/ng1550 Sakuntabhai A, Turbpaiboon C, Casadémont I, et al. A variant in the CD209 promoter is associated with severity of dengue disease[J]. Nat Genet, 2005, 37(5): 507-513.DOI: 10.1038/ ng1550

Alagarasu K Damle I M Bachal R V Association of promoter region polymorphisms of CD209 gene with clinical outcomes of dengue virus infection in Western India Infect Genet Evol 2013 17 239 242 10.1016/j.meegid.2013.04.024 Alagarasu K, Damle I M, Bachal R V, et al. Association of promoter region polymorphisms of CD209 gene with clinical outcomes of dengue virus infection in Western India[J]. Infect Genet Evol, 2013, 17: 239-242.DOI: 10.1016/j.meegid. 2013.04.024

Xavier-Carvalho C Gibson G Brasil P Single nucleotide polymorphisms in candidate genes and dengue severity in children: a case-control, functional and meta-analysis study Infect Genet Evol 2013 20 197 205 10.1016/j.meegid.2013.08.017 Xavier-Carvalho C, Gibson G, Brasil P, et al. Single nucleotide polymorphisms in candidate genes and dengue severity in children: a case-control, functional and meta-analysis study[J]. Infect Genet Evol, 2013, 20: 197-205. DOI: 10.1016/j.meegid.2013.08.017

Navarro-Sanchez E Altmeyer R Amara A Dendritic-cell-specific ICAM3-grabbing non-integrin is essential for the productive infection of human dendritic cells by mosquito-cell-derived dengue viruses EMBO Rep 2003 4 7 723 728 10.1038/sj.embor.embor866 Navarro-Sanchez E, Altmeyer R, Amara A, et al. Dendritic-cell-specific ICAM3-grabbing non-integrin is essential for the productive infection of human dendritic cells by mosquito-cell-derived dengue viruses[J]. EMBO Rep, 2003, 4(7): 723-728. DOI: 10.1038/sj. embor.embor866

Tassaneetrithep B Burgess T H Granelli-Piperno A DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells J Exp Med 2003 197 7 823 829 10.1084/jem.20021840 Tassaneetrithep B, Burgess T H, Granelli-Piperno A, et al. DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells[J]. J Exp Med, 2003, 197(7): 823-829. DOI: 10.1084/ jem.20021840

Richter M K da Silva Voorham J M Torres Pedraza S Immature dengue virus iIs infectious in human immature dendritic cells via interaction with the receptor molecule DC-SIGN PLoS One 2014 9 6 e98785 10.1371/journal.pone.0098785 Richter M K, da Silva Voorham J M, Torres Pedraza S, et al. Immature dengue virus iIs infectious in human immature dendritic cells via interaction with the receptor molecule DC-SIGN[J ] . PLoS One, 2014, 9(6): e98785.DOI: 10.1371/journal.pone.0098785

Roehrig J T Butrapet S Liss N M Mutation of the dengue virus type 2 envelope protein heparan sulfate binding sites or the domain Ⅲ lateral ridge blocks replication in Vero cells prior to membrane fusion Virology 2013 441 2 114 125 10.1016/j.virol.2013.03.011 Roehrig J T, Butrapet S, Liss N M, et al. Mutation of the dengue virus type 2 envelope protein heparan sulfate binding sites or the domain Ⅲ lateral ridge blocks replication in Vero cells prior to membrane fusion[J]. Virology, 2013, 441(2): 114-125. DOI: 10.1016/j.virol.2013.03. 011

王宇阎瑾琦张亮 DC-SIGN真核表达载体的构建及其稳定转染BHK21细胞系的建立解放军医学杂志 2010 35 3 304 306 http://www.cnki.com.cn/Article/CJFDTOTAL-JFJY201003030.htm 王宇,阎瑾琦,张亮,等. DC-SIGN真核表达载体的构建及其稳定转染BHK21细胞系的建立[J]. 解放军医学杂志, 2010,35(3): 304-306.

王欣谭磊陆凤 DC-SIGN真核表达载体的构建及其稳定转染HeLa细胞系的建立中国动物传染病学报 2015 23 3 12 16 10.3969/j.issn.1674-6422.2015.03.003 王欣,谭磊,陆凤,等. DC-SIGN真核表达载体的构建及其稳定转染HeLa细胞系的建立[J]. 中国动物传染病学报, 2015, 23(3): 12-16. DOI: 10.3969/j.issn.1674-6422.2015.03.003

朱守兵王泳高超人DC-SIGN基因克隆及其转基因细胞的构建苏州大学学报(医学版) 2010 30 1 102 105 http://www.cnki.com.cn/Article/CJFDTOTAL-SYXU201001030.htm 朱守兵,王泳,高超,等. 人DC-SIGN基因克隆及其转基因细胞的构建[J]. 苏州大学学报(医学版),2010, 30(1): 102-105.

Garderet L Cao H Salamero J In vitro production of dendritic cells from human blood monocytes for therapeutic use J Hematother Stem Cell Res 2001 10 4 553 567 10.1089/15258160152509163 Garderet L, Cao H, Salamero J, et al. In vitro production of dendritic cells from human blood monocytes for therapeutic use[J]. J Hematother Stem Cell Res, 2001, 10(4): 553-567, DOI: 10.1089/15258160152509163

Liu K Nussenzweig M C Origin and development of dendritic cells Immunol Rev 2010 234 1 45 54 10.1111/j.0105-2896.2009.00879.x Liu K, Nussenzweig M C.Origin and development of dendritic cells[J]. Immunol Rev,2010, 234(1): 45-54.DOI: 10.1111/j.0105-2896.2009. 00879.x

Romani N Gruner S Brang D Proliferating dendritic cell progenitors in human blood J Exp Med 1994 180 1 83 93 10.1084/jem.180.1.83 Romani N, Gruner S, Brang D, et al. Proliferating dendritic cell progenitors in human blood[J]. J Exp Med, 1994, 180(1): 83-93. DOI: 10.1084/jem.180.1.83

van de Laar L Coffer P J Woltman A M Regulation of dendritic cell development by GM-CSF: molecular control and implications for immune homeostasis and therapy Blood 2012 119 15 3383 3393 10.1182/blood-2011-11-370130 van de Laar L, Coffer P J, Woltman A M. Regulation of dendritic cell development by GM-CSF: molecular control and implications for immune homeostasis and therapy[J]. Blood, 2012, 119(15): 3383-3393. DOI: 10.1182/blood-2011-11- 370130

Barkhash A V Perelygin A A Babenko V N Single nucleotide polymorphism in the promoter region of the CD209 gene is associated with human predisposition to severe forms of tick-borne encephalitis Antiviral Res 2012 93 1 64 68 10.1016/j.antiviral.2011.10.017 Barkhash A V, Perelygin A A, Babenko V N, et al. Single nucleotide polymorphism in the promoter region of the CD209 gene is associated with human predisposition to severe forms of tick-borne encephalitis[J]. Antiviral Res, 2012, 93(1): 64-68. DOI: 10.1016/j.antiviral.2011.10. 017

方昕胡珍张俊磊登革1型病毒GZ2002株全基因组测序与分析中国现代医学杂志 2012 22 14 25 33 http://www.cnki.com.cn/Article/CJFDTOTAL-ZXDY201214005.htm 方昕,胡珍,张俊磊,等. 登革1型病毒GZ2002株全基因组测序与分析[J]. 中国现代医学杂志, 2012, 22(14):25-33.

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