SOCS3基因转染对肺腺癌细胞株A549原癌基因c-fos、c-jun mRNA表达及细胞增殖的影响

余祖滨; 白莉; 闵家新; 姚珂; 张国强

doi:10.16016/j.1000-5404.2004.17.016

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SOCS3基因转染对肺腺癌细胞株A549原癌基因c-fos、c-jun mRNA表达及细胞增殖的影响

更新时间：2026-01-09

- SOCS3基因转染对肺腺癌细胞株A549原癌基因c-fos、c-jun mRNA表达及细胞增殖的影响
- Journal of Army Medical University Vol. 26, Issue 17, (2004)
- 作者机构：
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- DOI：10.16016/j.1000-5404.2004.17.016
  CLC： R734.2
- Online First：15 September 2004，
  
  Published：2004
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余祖滨,白莉,闵家新,姚珂,张国强,SOCS3基因转染对肺腺癌细胞株A549原癌基因c-fos、c-jun mRNA表达及细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2004,26(17):0-.

余祖滨, 白莉, 闵家新, et al. SOCS3基因转染对肺腺癌细胞株A549原癌基因c-fos、c-jun mRNA表达及细胞增殖的影响[J]. Journal of Army Medical University, 2004, 26(17).
余祖滨,白莉,闵家新,姚珂,张国强,SOCS3基因转染对肺腺癌细胞株A549原癌基因c-fos、c-jun mRNA表达及细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2004,26(17):0-. DOI：10.16016/j.1000-5404.2004.17.016

余祖滨, 白莉, 闵家新, et al. SOCS3基因转染对肺腺癌细胞株A549原癌基因c-fos、c-jun mRNA表达及细胞增殖的影响[J]. Journal of Army Medical University, 2004, 26(17). DOI： 10.16016/j.1000-5404.2004.17.016.

摘要

目的　探讨SOCS3基因对人肺腺癌细胞株A5 49中c fos、c junmRNA表达及细胞增殖的影响。方法　以脂质体为载体将pEFSOCS3和pSV2neo共转染至人肺腺癌细胞株A5 49

RT PCR和免疫细胞化学法测定转染前后细胞中SOCS3mRNA和蛋白表达 ;半定量RT PCR测定转染前后细胞中c fos、c junmRNA水平表达变化 ;3H TdR掺入法检测基因转染前后细胞增殖情况。结果　RT PCR和免疫细胞化学证实转染后细胞中有SOCS3稳定表达 ;半定量RT PCR证实转染后细胞中c fos、c junmRNA水平显著降低 (P 0 .0 1) ;且转染组细胞 3H TdR掺入量显著降低 (P 0 .0 1)。结论　SOCS3蛋白可能通过负性调控STAT3介导的信号通路降低c fos、c jun的表达

从而抑制肺癌细胞增殖。

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