腺病毒介导gax基因转染抑制血管平滑肌细胞增殖

王耿; 何国祥; 徐晓辉; 冉擘力; 张萍; 陈渝

doi:10.16016/j.1000-5404.2003.24.007

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腺病毒介导gax基因转染抑制血管平滑肌细胞增殖

更新时间：2026-01-09

- 腺病毒介导gax基因转染抑制血管平滑肌细胞增殖
- Journal of Army Medical University Vol. 25, Issue 24, (2003)
- 作者机构：
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- DOI：10.16016/j.1000-5404.2003.24.007
  CLC： R543
- Online First：30 December 2003，
  
  Published：2003
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王耿,何国祥,徐晓辉,冉擘力,张萍,陈渝,腺病毒介导gax基因转染抑制血管平滑肌细胞增殖[J].陆军军医大学学报(原第三军医大学学报),2003,25(24):0-.

王耿, 何国祥, 徐晓辉, et al. 腺病毒介导gax基因转染抑制血管平滑肌细胞增殖[J]. Journal of Army Medical University, 2003, 25(24).
王耿,何国祥,徐晓辉,冉擘力,张萍,陈渝,腺病毒介导gax基因转染抑制血管平滑肌细胞增殖[J].陆军军医大学学报(原第三军医大学学报),2003,25(24):0-. DOI：10.16016/j.1000-5404.2003.24.007

王耿, 何国祥, 徐晓辉, et al. 腺病毒介导gax基因转染抑制血管平滑肌细胞增殖[J]. Journal of Army Medical University, 2003, 25(24). DOI： 10.16016/j.1000-5404.2003.24.007.

摘要

目的　以腺病毒介导gax基因转染血管平滑肌细胞 (VSMC)

探讨gax基因表达增强后VSMC增殖的变化。方法　以携带大鼠gax基因表达序列的复制缺陷型 5型腺病毒载体 (AdCMV gax)常规转染VSMC后

应用免疫细胞化学染色检测gax基因表达的变化 ;应用四唑盐 (MTT)比色试验、3H标记的胸腺嘧啶核苷 ( 3H TdR)掺入试验和流式细胞仪检测观察gax基因表达增强对VSMC增殖的影响。结果　①AdCMV gax转染前

PDGF BB下调Gax蛋白的表达

接近正常生理浓度的PDGF BB( 2ng ml)即可使VSMC的Gax蛋白表达率由 3 6.42 %显著降低至 2 2 .83 %(P 0 .0 5 )

随着PDGF BB浓度的升高

gax基因表达下降程度更加显著 ;AdCMV gax转染后

无论有无PDGF BB刺激

VSMC中gax基因的表达均比转染前显著增高。②AdCMV gax转染使VSMC表达gax增强后

VSMC的MTT吸光度值和3H TdR掺入量均较未转染组显著降低

G0 G1期的VSMC比例较未转染组显著增高 (P 0 .0 1)

G2 M期和S期细胞比例显著降低 (P 0 .0 1)。结论　增强gax基因表达可抑制由有丝分裂原刺激所引起的VSMC增殖

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Related Author

覃跃龙

舒茂琴

江明宏

王耿

何国祥

冉擘力

张萍

景涛

Related Institution

Department of Epidemiology, Faculty of Military Preventive Medicine, Army Medical University(Third Military Medical University)

Department of Thoracic Surgery, Second Affiliated Hospital of Army Medical University (Third Military Medical University)

Institute of Respiratory Diseases, Key Laboratory of Respiratory Diseases, Second Affiliated Hospital of Army Medical University (Third Military Medical University)

Department of Cardiology, Key Laboratory of Child Development and Disorders of Ministry of Education, International Science and Technology Cooperation Center of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University

Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University

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