Analyze the associativity between Helicobacter pylori infection and cholecystolithiasis

ZHU Yujuan

doi:10.13406/j.cnki.cyxb.2005.05.018

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Analyze the associativity between Helicobacter pylori infection and cholecystolithiasis

更新时间：2024-01-17

- Analyze the associativity between Helicobacter pylori infection and cholecystolithiasis
- Journal of Chongqing Medical University Issue 5, (2005)
- 作者机构：
  
  1. 成都三六三医院消化内科
  2. 四川省卫生干部管理学院
  3. 四川大学华西医院,成都,610041
  4. 成都三六三医院消化内科四川省卫生干部管理学院四川大学华西医院,成都,610041
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.05.018
  CLC： R575.6
- Published：2005，
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[1]朱玉娟,谢健,周卫东,肖思洁,杨华,李石沙,李燕,陶传敏.幽门螺杆菌感染与胆囊结石形成的相关性分析[J].重庆医科大学学报,2005(05):68-70.

ZHU Yujuan. Analyze the associativity between Helicobacter pylori infection and cholecystolithiasis. [J]. Journal of Chongqing Medical University (5).(2005)
[1]朱玉娟,谢健,周卫东,肖思洁,杨华,李石沙,李燕,陶传敏.幽门螺杆菌感染与胆囊结石形成的相关性分析[J].重庆医科大学学报,2005(05):68-70. DOI： 10.13406/j.cnki.cyxb.2005.05.018.

ZHU Yujuan. Analyze the associativity between Helicobacter pylori infection and cholecystolithiasis. [J]. Journal of Chongqing Medical University (5).(2005) DOI： 10.13406/j.cnki.cyxb.2005.05.018.

摘要

目的:研究和探讨幽门螺杆菌（Helicobacter pylori

Hp）与胆囊结石形成关系

寻找Hp在胆道中存在的证据

同时对胆道中HpDNA的检测方法进行研究和分析。方法:参照文献对120例胆囊结石患者胆汁、粘膜和胆石进行Hp培养

并以Ure A和cagA作引物进行扩增

对扩增产物进行分析。结果:120例样本检测均为阴性结果

培养无Hp生长

使用PCR方法未检测到Hp DNA及Hp CagA抗体。结论:（1）运用PCR方法在胆汁和胆石中检测HpDNA尚受到多种因素制约。（2）Hp可能经Oddi′s括约肌逆行的进入胆道但与胆囊结石并无明确相关性。

Abstract

Objective:To investigate the relationship of H pylori with cholecystolithiasis

to collect the evidence of Hp existing in biliary tract

and to study the detective method of Hp DNA in billiary tract.Methods:Hp which existing in bile

mucosa and gallstone of 120 cholecystolithiasis patients were cultured according to the literature.Then they were amplified by the primer of Ure A and cagA.Amplified products were analyzed.Results:All of the 120 samples were negative.No Hp grew in the medium.No Hp DNA and Hp Cag A antibody were found with PCR method.Conclusion:(1) It was restricted by many factors that Hp DNA in bile and gallstone wereexamined with PCR.(2) Hp maybe entered biliary tract regressively through Oddi’s sphincter andhad no relationship with cholecystolithiasis.

关键词

幽门螺杆菌胆囊结石PCRHpCagA

Keywords

Helicobacter pyloricholecystolithiasisPCRHp Cag A

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