The effect of methylmercury on the epithelial apoptosis of fetal mice intestine in vitro

ZHANG Haiying; et al

doi:10.13406/j.cnki.cyxb.2005.05.011

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The effect of methylmercury on the epithelial apoptosis of fetal mice intestine in vitro

更新时间：2024-01-17

- The effect of methylmercury on the epithelial apoptosis of fetal mice intestine in vitro
- Journal of Chongqing Medical University Issue 5, (2005)
- 作者机构：
  
  1. 重庆医科大学基础医学院组织与胚胎学教研室
  2. 重庆医科大学基础医学院组织与胚胎学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.05.011
  CLC： X174
- Published：2005，
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[1]张海英,汪维伟,姜蓉.甲基汞影响小鼠胚胎肠上皮凋亡的体外实验[J].重庆医科大学学报,2005(05):43-46.

ZHANG Haiying, et al. The effect of methylmercury on the epithelial apoptosis of fetal mice intestine in vitro. [J]. Journal of Chongqing Medical University (5).(2005)
[1]张海英,汪维伟,姜蓉.甲基汞影响小鼠胚胎肠上皮凋亡的体外实验[J].重庆医科大学学报,2005(05):43-46. DOI： 10.13406/j.cnki.cyxb.2005.05.011.

ZHANG Haiying, et al. The effect of methylmercury on the epithelial apoptosis of fetal mice intestine in vitro. [J]. Journal of Chongqing Medical University (5).(2005) DOI： 10.13406/j.cnki.cyxb.2005.05.011.

摘要

目的:观察低剂量氯化甲基汞（methylmercury chloride

MMC）对胎鼠肠上皮细胞凋亡及凋亡相关蛋白表达的影响。方法:分别取E13.5d、E14.5d胎鼠的十二指肠及结肠

体外培养

分别加入MMC液（0μg/L

200μg/L

400μg/L

800μg/L）

培养24h后取出标本

制成石蜡切片。部分切片经HE染色

体视学法计数肠上皮中的凋亡小体;余切片经免疫组化染色

观察凋亡相关蛋白Bcl-2

Bax

P53及c-Fos的表达。结果:①实验各组肠上皮凋亡小体数均较对照组高

P<0.05

与MMC剂量呈正相关

P<0.01。②Bcl-2在各实验组胎鼠肠上皮细胞的表达均低于对照组

P<0.05

与MMC的剂量呈负相关

P<0.01;而Bax、P53及c-Fos的表达则高于对照组

P<0.05

与MMC剂量均呈正相关

P<0.01。结论:MMC在体外可促进胎鼠肠上皮细胞凋亡

可能是一种通过下调Bcl-2表达

上调Bax及c-Fos表达的机制。

Abstract

Objective:To study the effect of methylmercury chloride(MMC) on the apoptosis and the expression of apoptosis-associated proteins in the intestinal epithelium of fetal mice.Methods:The duodenum and colon were taken from E13.5d and E14.5d fetal mice respectively

and cultured with different doses of MMC(0 μg/L

200μg/L

400μg/L

800μg/L) for 24h.The samples were sliced into the paraffin sections.Some sections were stained with HE to count the numbers of apoptotic bodies(NAB) in the fetal intestinal epithelium with stereological method.The others were stained with the immunohistochemical method to observe the expression of apoptosis-associated proteins Bcl-2

Bax

P53 and c-Fos.Results:①In experimental groups

all the NAB in the intestinal epithelium were higher than that in control groups(P<0.05)

and there was a positive correlation between the doses of MMC and the NAB(P<0.01).②In all experimental groups

the expression of Bcl-2 in the intestinal epithelium was lower than that in control groups(P<0.05)

and there was a negative correlation between the doses of MMC and the expression of Bcl-2(P<0.01).However the expression of Bax

P53 and c-Fos were higher than that in control groups(P<0.05).There was a positive correlation between the doses of MMC and the expression of these proteins(P<0.01).Conclusion:MMC can increase the intestinal epithelium-apoptosis in fetal mice in vitro

whose mechanism may be restraining the expression of Bcl-2 and promoting the expression of Bax and c-Fos.

关键词

氯化甲基汞胎鼠肠上皮凋亡

Keywords

Methylmercury chlorideFetal mouseIntestinal epitheliumapoptosis

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