Expression and significance of heme oxygenase-1 in delayed cardiac xenograft rejection

LIU Shengchun; et al

doi:10.13406/j.cnki.cyxb.2005.05.007

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Expression and significance of heme oxygenase-1 in delayed cardiac xenograft rejection

更新时间：2024-01-17

- Expression and significance of heme oxygenase-1 in delayed cardiac xenograft rejection
- Journal of Chongqing Medical University Issue 5, (2005)
- 作者机构：
  
  1. 重庆医科大学附属第一医院普外科
  2. 重庆医科大学附属第一医院普外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.05.007
  CLC： R654.2
- Published：2005，
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[1]刘胜春,姚榛祥,孙正魁,唐华,董蒲江.HO-1在延迟性异种心脏移植排斥反应中的表达及意义[J].重庆医科大学学报,2005(05):29-33.

LIU Shengchun, et al. Expression and significance of heme oxygenase-1 in delayed cardiac xenograft rejection. [J]. Journal of Chongqing Medical University (5).(2005)
[1]刘胜春,姚榛祥,孙正魁,唐华,董蒲江.HO-1在延迟性异种心脏移植排斥反应中的表达及意义[J].重庆医科大学学报,2005(05):29-33. DOI： 10.13406/j.cnki.cyxb.2005.05.007.

LIU Shengchun, et al. Expression and significance of heme oxygenase-1 in delayed cardiac xenograft rejection. [J]. Journal of Chongqing Medical University (5).(2005) DOI： 10.13406/j.cnki.cyxb.2005.05.007.

摘要

目的:探讨血红素氧合酶-1在延迟性异种心脏移植排斥反应中的表达及意义。方法:建立NIH-Wistar颈部异位心脏移植模型

分别于移植前、移植后1h、6h、12h、24h、48h和72h切取移植心脏

应用RT-PCR、Western Blot检测HO-1mRNA、HO-1蛋白表达并检测HO-1酶活性;同时比较钴原卟啉（CoPP）诱导HO-1对延迟性异种心脏移植排斥反应的影响。结果:移植心脏均有HO-1表达

HO-1mRNA（t=2.5170

0.05）、HO-1蛋白表达（t=2.3702

0.05）及酶活性（t=2.246

0.05）移植术后24

8h表达到达高峰;CoPP诱导的HO-1明显延长了移植心脏的存活时间（t=4.74442

0.001）。结论:HO-1表达在延迟性异种心脏移植后24

8h到达高峰;CoPP诱导的HO-1能延长延迟性异种心脏移植的存活时间。

Abstract

Objective:To investigate the expression and significance of heme oxygenase-1(HO-1) in delayed cardiac xenograft rejection in NIH-Wistar.Methods:The model of cervical heterotopic heart transplantation in NIH-Wistar was established.The hearts were harvested 1

48 as well as 72 hours before and after transplantion respectively.HO-1mRNA and HO-1 protein were examined by RT-PCR and Western Blot

HO-1 enzymatic activity was examined.The survival time of transplanted heart

in which HO-1 was induced by cobalt protoporphyrin(CoPP)

was compared with the control.Results:HO-1 was expressed in transplanted heart.The expression of HO-1mRNA(t=2.5170

P<0.05)

protein(t=2.3702

P<0.05) and enzymatic activity(t=2.246

P<0.05)in heart 24 and 48 hours after transplantion were higher than those in other groups.Conclusion:The expression peak of HO-1 in NIH-Wistar cardiac transplantation is 24～48 hours after transplantion.HO-1 induced by CoPP can prolong the survival time of delayed xenograft cardiac transplantation.

关键词

血红素氧合酶-1延迟性异种移植排斥反应心脏

Keywords

Heme oxygenase-1Delayed xenograft rejectionheart

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