Effects of dendritic cells pulsed with autologous hepatoma cell lysates on the immune function of the postoperative patients with hepatocellular carcinoma

GAO Jian; et al

doi:10.13406/j.cnki.cyxb.2005.05.005

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Effects of dendritic cells pulsed with autologous hepatoma cell lysates on the immune function of the postoperative patients with hepatocellular carcinoma

更新时间：2024-01-17

- Effects of dendritic cells pulsed with autologous hepatoma cell lysates on the immune function of the postoperative patients with hepatocellular carcinoma
- Journal of Chongqing Medical University Issue 5, (2005)
- 作者机构：
  
  1. 重庆医科大学附属第二医院肝病治疗中心
  2. 重庆医科大学附属第二医院肝病治疗中心,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.05.005
  CLC： R735.7
- Published：2005，
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[1]高建,陈敏,任红.人自体肝癌细胞裂解物致敏的DC瘤苗对肝癌术后免疫功能的影响[J].重庆医科大学学报,2005(05):22-25.

GAO Jian, et al. Effects of dendritic cells pulsed with autologous hepatoma cell lysates on the immune function of the postoperative patients with hepatocellular carcinoma. [J]. Journal of Chongqing Medical University (5).(2005)
[1]高建,陈敏,任红.人自体肝癌细胞裂解物致敏的DC瘤苗对肝癌术后免疫功能的影响[J].重庆医科大学学报,2005(05):22-25. DOI： 10.13406/j.cnki.cyxb.2005.05.005.

GAO Jian, et al. Effects of dendritic cells pulsed with autologous hepatoma cell lysates on the immune function of the postoperative patients with hepatocellular carcinoma. [J]. Journal of Chongqing Medical University (5).(2005) DOI： 10.13406/j.cnki.cyxb.2005.05.005.

摘要

目的:探讨人自体肝癌细胞裂解物致敏的树突状细胞（DC）瘤苗对肝癌术后免疫功能的影响。方法:从肝癌患者外周血单个核细胞中诱导DC

用重组人粒细胞-巨噬细胞集落刺激因子（rhGM-CSF）和白介素-4（rhIL-4）刺激活化

经自体肝癌细胞裂解物致敏制备DC瘤苗。将30例肝癌术后患者随机分为DC瘤苗治疗组15例

化疗对照组15例。对两组病例治疗前后免疫功能、临床疗效进行观察比较。结果:DC瘤苗治疗后外周血DC3+、DC4+/CD8+及NK细胞比率较治疗前明显升高

P<0.05

且明显高于对照组化疗后的DC3+、DC4+/CD8+及NK细胞比率;DC瘤苗治疗后外周血IgGI、gM、IgA等体液免疫指标较治疗前有一定程度的改善

但差异无显著性

P>0.05;DC瘤苗治疗后血清IL-10水平明显下降

P<0.05。结论:肝癌术后行自体肝癌细胞裂解物致敏的DC瘤苗治疗

可改善患者的细胞免疫和体液免疫水平

且不良反应少

值得推广应用。

Abstract

Objective:To investigate effects of dendritic cells(DCs) pulsed with autologous hepatoma cell lysates on the immune function of the postoperative patients with hepatocellular carcinoma(HCC).Methods:DCs isolated from peripheral blood mononuclear cell of HCC patients were cultured and proliferated in vitro by using rhGM-CSF and rhIL-4

and then were pulsed with autologous hepatoma cell lysates.30 postoperative patients with HCC were randomly divided into 2 groups.15 cases were treated with dendritic cell vaccine.15 cases received only chemotherapy and were established as control group.Immune function、clinical effects in two groups of patients were compared and observed.Results: The levels of DC3~+、DC4~+/CD8~+ and NK cell in the DC vaccine group significantly increased after vaccination

while those of the control group had no significant changes.The levels of IgG、IgM、IgA in the DC vaccine group increased after vaccination

but didn’t reach statistical significance.The concentration of IL-10 in the DC vaccine group significantly decreased after vaccination(P<0.05).Conclusion:The hopatome cell lysates pulsed DC vaccines can improve the immune function of the postoperative patients with HCC.This method results in fewer side effects and should be recommended.

关键词

树突状细胞原发性肝癌免疫功能免疫治疗

Keywords

Dendritice cellhepatocellular carcinomaimmune functionimmunotherapy

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