Bone marrow mesenchymal stem cells to express cardiogenic phenotype in a coculture system

您当前的位置：

首页 >

文章列表页 >

Bone marrow mesenchymal stem cells to express cardiogenic phenotype in a coculture system

更新时间：2024-01-17

- Bone marrow mesenchymal stem cells to express cardiogenic phenotype in a coculture system
- Journal of Chongqing Medical University Issue 5, (2005)
- 作者机构：
  
  1. 重庆医科大学附属第二医院心内科
  2. 重庆医科大学附属第一医院心内科
  3. 重庆医科大学附属第二医院心内科,重庆,400010
  4. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.05.004
  CLC： R541
- Published：2005，
扫描看全文
[1]苏立,陈运贞,张晓刚,佘强.在心肌细胞/MSCs共培养体系中MSCs出现心肌分化表型[J].重庆医科大学学报,2005(05):18-21+25.

SU Li, et al. Bone marrow mesenchymal stem cells to express cardiogenic phenotype in a coculture system. [J]. Journal of Chongqing Medical University (5).(2005)
[1]苏立,陈运贞,张晓刚,佘强.在心肌细胞/MSCs共培养体系中MSCs出现心肌分化表型[J].重庆医科大学学报,2005(05):18-21+25. DOI： 10.13406/j.cnki.cyxb.2005.05.004.

SU Li, et al. Bone marrow mesenchymal stem cells to express cardiogenic phenotype in a coculture system. [J]. Journal of Chongqing Medical University (5).(2005) DOI： 10.13406/j.cnki.cyxb.2005.05.004.

摘要

目的:观察MSCs在心肌细胞/MSCs共培养体系中向心肌细胞的分化情况。方法:体外分离培养大鼠心肌细胞

然后将传代后大鼠MSCs与心肌细胞混合培养

建立心肌细胞/MSCs共培养体系

同时以心肌细胞培养上清液及成纤维细胞培养上清液为诱导因素作对照。培养1周后行免疫细胞化学染色

鉴定MSCs中心肌特异性cTnT蛋白的表达。结果:在心肌细胞/MSCs共培养体系中

MSCs与心肌细胞交织成网状

部分MSCs出现cTnT的表达

而对照组MSCs未检出cTnT的表达。结论:体外模拟的心肌环境可以诱导MSCs向心肌细胞分化并表达心肌细胞特异性标志物

诱导MSCs向心肌分化的分子信号可能是心肌细胞膜蛋白。

Abstract

Objective:To observe the tansdifferentiation of bone marrow mesenchymal stem cells(MSCs) into cardiomyocyte in a coculture system with cardiomyocytes.Methods:Newly born Wistar Rats’ cardiomyocytes were isolated and cultured and the cells were labeled with DAPI.Prepared MSCs were planted into the cardiomyocytes dishes to establish a co-culture system.Supernatant liquid of the cardiomyocytes or fibroblast was added the MSCs(non-labeled) dishes as the control. After one week of coculture

fluorescence microscope observation and immunocytochemical staining with cTnT was performed to evaluate the phenotype changes in non-labeled MSCs.Results:MSCs and cardiomyocytes were connect with each other by net in culture plate after a few days of coculture;some non-labeled cells were positively stained by cTnT antibody while the MSCs cultured with cardiomyocytes or fibroblast supernatant liquid were stained negatively.Conclusion:To obtain cardiomyocyte phenotype in vitro

direct contact of MSCs with cardiomyocytes is essential.The signaling molecules induce MSCs’ transformation into cardiomyocytes are likely some membrane proteins on the surface of cardiomyocytes.

关键词

间充质干细胞诱导细胞分化共培养

Keywords

mesenchymal stem cellsInducecell differentiationCoculture

references

0

Views

122

下载量

3

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Related Articles

Research advances in the therapeutic effect of mesenchymal stem cell exosomes on pulmonary hypertension

EXPERIMENTAL RESEARCH OF BONE INDUCIBLE CAPACITY AND DIFFERENCE OF BONE MATRIX GELATIN AND DECALFIED BONE MATRIX

5-azacytidine induce bone marrow mesenchymal stem cell differentiation into cardiomyocyte-like cells in vitro

The study about the cardiac differentiation of human mesenchymal stem cells induced by rat cardiomyocytes

Immunoregulation of mesenchymal stem cells from the patients with idiopathic thrombocytopenic purpura

Related Author

Ge Rili

Ma Lan

Zhang Qingqing

First Hospital

SU Li

马旭

张润峰

张晓刚

Related Institution

Research Center for High Altitude Medicine， Qinghai University；Key Laboratory of High Altitude Medicine， Ministry of Education；Key Laboratory of Application and Foundation for High Altitude Medicine Research in Qinghai Province

Hu Xiaobo Wu Zuyao

重庆医科大学临床学院肾内科

Department of Cardiology,College of Clinical Medicine,Chongqing Medical University

垫江县妇幼保健院

⁰