Effects of anti-human augmenter of liver regeneration monoclonal antibody on proliferation of HepG2 cells in vitro

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Effects of anti-human augmenter of liver regeneration monoclonal antibody on proliferation of HepG2 cells in vitro

更新时间：2024-01-17

- Effects of anti-human augmenter of liver regeneration monoclonal antibody on proliferation of HepG2 cells in vitro
- Journal of Chongqing Medical University Issue 5, (2005)
- 作者机构：
  
  1. 重庆医科大学病毒性肝炎研究所教育部感染性疾病分子生物学重点实验室
  2. 四川省医学科学院-四川省人民医院感染科
  3. 重庆医科大学病毒性肝炎研究所教育部感染性疾病分子生物学重点实验室,重庆,400010
  4. ,成都,610072
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.05.001
  CLC： R735.7
- Published：2005，
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[1]唐琳,孙航,张林,郭晖,陈雪华,邓建川,刘杞.抗人肝再生增强因子单克隆抗体对人肝癌细胞株HepG2增殖活性的影响[J].重庆医科大学学报,2005(05):7-9+17.

TANG Lin, et al. Effects of anti-human augmenter of liver regeneration monoclonal antibody on proliferation of HepG2 cells in vitro. [J]. Journal of Chongqing Medical University (5).(2005)
[1]唐琳,孙航,张林,郭晖,陈雪华,邓建川,刘杞.抗人肝再生增强因子单克隆抗体对人肝癌细胞株HepG2增殖活性的影响[J].重庆医科大学学报,2005(05):7-9+17. DOI： 10.13406/j.cnki.cyxb.2005.05.001.

TANG Lin, et al. Effects of anti-human augmenter of liver regeneration monoclonal antibody on proliferation of HepG2 cells in vitro. [J]. Journal of Chongqing Medical University (5).(2005) DOI： 10.13406/j.cnki.cyxb.2005.05.001.

摘要

目的:检测人肝癌细胞株HepG2细胞有无人肝再生增强因子（hALR）的表达

利用hALR特异性的单克隆抗体（anti-hALR McAb）结合hALR

观察其阻断hALR的作用后

对人肝癌细胞株HepG2细胞的增殖活性的影响。方法:用免疫细胞化学以及RT-PCR的方法检测HepG2细胞hALR蛋白及mRNA的表达;用3H-TdR掺入法检测hALR特异性单克隆抗体中和hALR后HepG2细胞的增殖情况。结果:①HepG2细胞有hALR的表达。②抗hALR单克隆抗体特异性地阻断hALR的作用后可部分抑制肿瘤细胞自主性生长。结论:抗hALR单克隆抗体能部分抑制肿瘤细胞自主性生长;hALR通过自分泌方式参与了肝癌细胞的自主性生长。

Abstract

Objective:To detect the existence of expression of human augmenter of liver regeneration(hALR) in HepG2 cells

and to observe proliferation of HepG2 cells after neutralizing hALR with anti-hALR McAb.Methods:the expression of hALR in HepG2 cells was observed immunocytochemistry and reverse transcriptive PCR(RT-PCR).Proliferation of HepG2 cells after neutralization was detected by ~3?H-TdR incorporation approach.Results:① hALR was expressed by HepG2 cells.② Anti-hALR McAb inhibited partially the autonomous growth of HepG2 cells.Conclusion:Anti-hALR McAb inhibit the autonomous growth of hepatoma cells partially;moreover

hALR maintain the autonomous growth of hepatoma cells in vitro through autocrine mechanism.

关键词

人肝再生增强因子单克隆抗体肝癌细胞株

Keywords

Human augmenter of liver regenerationmonoclonal antibodyhepatocellular carcinomaCell line

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