Effect of dandelion polysaccharide on inflammatory response and expression of S100 calcium binding protein A8/A9 in lung and intestinal injuries in rats with multiple organ dysfunction syndrome

Li Han; Yu Wenlong; Yang Zhaofeng; Liu Yan; Zhang Qian; Wang Jianhui; Zhu Liyan; Zuo Jiehui; Yang Xiuhong

doi:10.13406/j.cnki.cyxb.003908

您当前的位置：

首页 >

文章列表页 >

Effect of dandelion polysaccharide on inflammatory response and expression of S100 calcium binding protein A8/A9 in lung and intestinal injuries in rats with multiple organ dysfunction syndrome

Basic research | 更新时间：2025-10-21

- Effect of dandelion polysaccharide on inflammatory response and expression of S100 calcium binding protein A8/A9 in lung and intestinal injuries in rats with multiple organ dysfunction syndrome
- Journal of Chongqing Medical University Vol. 50, Issue 10, Pages: 1399-1407(2025)
- 作者机构：
  
  华北理工大学基础医学院，唐山 063210
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.003908
  CLC： R3
- Received：18 April 2025，
  
  Revised：2025-05-27，
  
  Accepted：24 June 2025，
  
  Published：28 October 2025
- 稿件说明：
移动端阅览
李涵,于文龙,杨兆丰,等.蒲公英多糖对多器官功能障碍综合征大鼠肺肠损伤炎症反应及S100A8/A9表达的影响[J].重庆医科大学学报,2025,50(10):1399-1407.

Li Han,Yu Wenlong,Yang Zhaofeng,et al.Effect of dandelion polysaccharide on inflammatory response and expression of S100 calcium binding protein A8/A9 in lung and intestinal injuries in rats with multiple organ dysfunction syndrome[J].Journal of Chongqing Medical University,2025,50(10):1399-1407.
李涵,于文龙,杨兆丰,等.蒲公英多糖对多器官功能障碍综合征大鼠肺肠损伤炎症反应及S100A8/A9表达的影响[J].重庆医科大学学报,2025,50(10):1399-1407. DOI： 10.13406/j.cnki.cyxb.003908.

Li Han,Yu Wenlong,Yang Zhaofeng,et al.Effect of dandelion polysaccharide on inflammatory response and expression of S100 calcium binding protein A8/A9 in lung and intestinal injuries in rats with multiple organ dysfunction syndrome[J].Journal of Chongqing Medical University,2025,50(10):1399-1407. DOI： 10.13406/j.cnki.cyxb.003908.

摘要

目的

观察蒲公英多糖（dandelion polysaccharide，DP）对大鼠多器官功能障碍综合征（multiple organ dysfunction syndrome，MODS）肺、小肠组织炎症反应及S100钙结合蛋白A8/A9（S100 calcium-binding protein A8/A9，S100A8/A9）表达的影响。

方法

应用失血性休克及腹腔注射脂多糖“二次打击”的方法，构建大鼠MODS模型，分为假手术组、模型组、DP低剂量组、DP高剂量组。观察各组大鼠肺和小肠脏器系数及湿/干重比值；苏木精-伊红（hematoxylin-eosinstaining，HE）染色观察肺和小肠组织病理形态学改变；免疫组织化学染色观察肺和小肠组织白细胞介素（interleukin，IL）1β、IL-6、IL-10的表达；蛋白质免疫印迹法（Western blot）检测肺和小肠组织S100A8/A9蛋白表达变化。

结果

与假手术组相比，模型组大鼠肺脏系数（5.849±0.824）、湿/干重比值（6.556±0.631）和小肠湿/干重比值（6.356±0.535）、湿重/长度比值（73.950±5.569）均上升。HE染色显示肺泡腔及肺间质可见大量炎细胞浸润，肺泡壁增厚；小肠绒毛崩解碎裂，可见炎细胞浸润及节段性集合淋巴滤泡增生。S100A8/A9主要表达于中性粒细胞和巨噬细胞，大鼠肺和小肠组织S100A8/A9、IL-1β、IL-6表达上调，IL-10表达下调。给予高剂量DP治疗后，大鼠肺脏系数（4.297±0.462）、湿/干重比值（5.313±0.495）和小肠湿/干重比值（5.398±0.388）、湿重/长度比值（59.417±2.891）均下降。肺和小肠组织病理损伤减轻，肺和小肠组织S100A8/A9、IL-1β、IL-6表达下调，IL-10表达上调。

结论

DP可能通过抑制S100A8/A9的表达，减轻大鼠MODS肺和小肠组织损伤的炎症反应。

Abstract

Objective

To investigate the effect of dandelion polysaccharide（DP） on inflammatory response and the protein expression of S100 calcium binding protein A8/A9（S100A8/A9） in lung tissue and small intestinal tissue of rats with multiple organ dysfunction syndrome （MODS）.

Methods

The two-hit method of hemorrhagic shock and intraperitoneally injected lipopolysaccharide was used to establish a rat model of MODS，and the rats were divided into sham-operation group，model group，low-dose DP group，and high-dose DP group. The organ coefficient and wet/dry weight ratio of the lung and the small intestine were observed for each group of rats； HE staining was used to observe the pathomorphological changes of lung tissue and small intestinal tissue； immunohistochemical staining was used to measure the expression of interleukin-1β（IL-1β），interleukin-6 （IL-6），and interleukin-10（IL-10） in lung tissue and small intestinal tissue； Western blot was used to measure the protein expression level of S100A8/A9 in lung tissue and small intestinal tissue.

Results

Compared with the sham-operation group，the model group had significant increases in the organ coefficient of the lung（5.849±0.824），the wet/dry weight ratio of the lung（6.556±0.631），the wet/dry weight ratio of the small intestine（6.356±0.535），and the wet weight/length ratio of the small intestine（73.950±5.569）. HE staining showed that that the model group had massive inflammatory cell infiltration in alveolar space and pulmonary interstitium，thickened alveolar wall，and disintegration and fragmentation of the villi of the small intestine，with inflammatory cell infiltration and proliferation of segmental aggregated lymphoid follicles. In the model group，S100A8/A9 was mainly expressed in neutrophils and macrophages，and there were increases in the expression of S100A8/A9，IL-1β，and IL-6 and a reduction in the expression of IL-10 in the lung tissue and small intestinal tissue of rats. After treatment with high-dose DP，there were reductions in the organ coefficient of the lung（4.297±0.462），the wet/dry weight ratio of the lung （5.313±0.495），the wet/dry weight ratio of the small intestine（5.398±0.388），and the wet weight/length ratio of the small intestine （59.417±2.891）. The high-dose group also had alleviation of pathological injury in the small intestine，with reductions in the expression of S100A8/A9，IL-1β，and IL-6 and an increase in the expression of IL-10 in lung tissue and small intestinal tissue.

Conclusion

DP may alleviate inflammatory response in lung and small intestinal injuries of rats with MODS by inhibiting the expression of S100A8/A9.

关键词

Keywords

references

Gourd NM ， Nikitas N . Multiple organ dysfunction syndrome [J ] . J Intensive Care Med ， 2020 ， 35 （ 12 ）： 1564 - 1575 ．

Li R ， Ye JJ ， Gan LB ， et al . Traumatic inflammatory response：pathophysiological role and clinical value of cytokines [J ] . Eur J Trauma Emerg Surg ， 2024 ， 50 （ 4 ）： 1313 - 1330 ．

Cohen J ， Vincent JL ， Adhikari NKJ ， et al . Sepsis：a roadmap for future research [J ] . Lancet Infect Dis ， 2015 ， 15 （ 5 ）： 581 - 614 ．

Tang F ， Zhao XL ， Xu LY ， et al . Endothelial dysfunction：Pathophysiology and therapeutic targets for sepsis-induced multiple organ dysfunction syndrome [J ] . Biomed Pharmacother ， 2024 ， 178 ： 117180 ．

Lv K ， Song J ， Wang J ， et al . Pterostilbene alleviates dextran sodium sulfate（DSS）-induced intestinal barrier dysfunction involving suppression of a S100A8-TLR-4-NF-κB signaling cascade [J ] . J Agric Food Chem ， 2024 ， 72 （ 33 ）： 18489 - 18496 ．

Pruenster M ， Vogl T ， Roth J ， et al . S100A8/A9：From basic science to clinical application [J ] . Pharmacol Ther ， 2016 ， 167 ： 120 - 131 ．

Nakata K ， Okazaki M ， Sakaue T ， et al . Functional blockage of S100A8/A9 ameliorates ischemia-reperfusion injury in the lung [J ] . Bioengineering （Basel）， 2022 ， 9 （ 11 ）： 673 ．

Du L ， Chen YB ， Shi JS ， et al . Inhibition of S100A8/A9 ameliorates renal interstitial fibrosis in diabetic nephropathy [J ] . Metabolism ， 2023 ， 144 ： 155376 ．

Li M ， Zhang HN ， Hu XY ， et al . Isolation of a new polysaccharide from dandelion leaves and evaluation of its antioxidant，antibacterial，and anticancer activities [J ] . Molecules ， 2022 ， 27 （ 21 ）： 7641 ．

Li Z ， Li XY ， Shi PP ， et al . Modulation of acute intestinal inflammation by dandelion polysaccharides：an in-depth analysis of antioxidative，anti-inflammatory effects and gut microbiota regulation [J ] . Int J Mol Sci ， 2024 ， 25 （ 3 ）： 1429 ．

Yang N ， Li C ， Tian G ， et al . Organic acid component from Taraxacum mongolicum Hand. -Mazz alleviates inflammatory injury in lipopolysaccharide-induced acute tracheobronchitis of ICR mice through TLR4/NF-κB signaling pathway [J ] . Int Immunopharmacol ， 2016 ， 34 ： 92 - 100 ．

Aziz M ， Ode Y ， Zhou M ， et al . B-1a cells protect mice from sepsis-induced acute lung injury [J ] . Mol Med ， 2018 ， 24 （ 1 ）： 26 ．

Xu N ， Zhang CP ， Jing LL ， et al . Protective effect and mechanism of rebamipide on NSAIDs associated small bowel injury [J ] . Int Immunopharmacol ， 2021 ， 90 ： 107136 ．

Zhao T ， Zhang Y ， Nan LH ， et al . Impact of structurally diverse polysaccharides on colonic mucin O-glycosylation and gut microbiota [J ] . NPJ Biofilms Microbiomes ， 2023 ， 9 （ 1 ）： 97 ．

Gibson BHY ， Wollenman CC ， Moore-Lotridge SN ， et al . Plasmin drives burn-induced systemic inflammatory response syndrome [J ] . JCI Insight ， 2021 ， 6 （ 23 ）： e154439 ．

Rybakova MG . Sepsis：from systemic inflammatory response syndrome to organ dysfunction [J ] . Arkh Patol ， 2021 ， 83 （ 1 ）： 67 - 72 ．

Liu Y ， Shi YJ ， Zou JB ， et al . Extraction，purification，structural features，biological activities，modifications ，and applications from Taraxacum mongolicum polysaccharides：a review [J ] . Int J Biol Macromol ， 2024 ， 259 ： 129193 ．

Yan SK ， Dong R . Integrated microbiome-metabolomics analysis reveals the potential mechanism of dandelion root polysaccharides to ameliorate ulcerative colitis [J ] . Metabolites ， 2024 ， 14 （ 7 ）： 351 ．

Zhou SS ， Wang Z ， Hao YL ， et al . Dandelion polysaccharides ameliorate high-fat-diet-induced atherosclerosis in mice through antioxidant and anti-inflammatory capabilities [J ] . Nutrients ， 2023 ， 15 （ 19 ）： 4120 .

Hu C ， Kitts DD . Luteolin and luteolin-7-O-glucoside from dandelion flower suppress iNOS and COX-2 in RAW 264.7 cells [J ] . Mol Cell Biochem ， 2004 ， 265 （ 1/2 ）： 107 - 113 ．

Liang WF ， Li HT ， Zhou HY ， et al . Effects of Taraxacum and Astragalus extracts combined with probiotic Bacillus subtilis and Lactobacillus on Escherichia coli -infected broiler chickens [J ] . Poult Sci ， 2021 ， 100 （ 4 ）： 101007 ．

Lee W ， Lee CH ， Lee J ， et al . Botanical formulation，TADIOS，alleviates lipopolysaccharide（LPS）-induced acute lung injury in mice via modulation of the Nrf2-HO-1 signaling pathway [J ] . J Ethnopharmacol ， 2021 ， 270 ： 113795 ．

兰　平，张宗进，何　真 . 炎症性肠病的外科治疗进展 [J ] . 中华消化外科杂志， 2024 ， 23 （ 6 ）： 775 - 781 .

Lan P ， Zhang ZJ ， He Z . Progress in surgical treatment of inflammatory bowel disease [J ] . Chin J Dig Surg ， 2024 ， 23 （ 6 ）： 775 - 781 .

Wang S ， Song R ， Wang Z ， et al . S100A8/A9 in inflammation [J ] . Front Immunol ， 2018 ， 9 ： 1298 ．

Revenstorff J ， Ludwig N ， Hilger A ， et al . Role of S100A8/A9 in platelet-neutrophil complex formation during acute inflammation [J ] . Cells ， 2022 ， 11 （ 23 ）： 3944 ．

van Kooten NJT ， Blom AB ， Teunissen van Manen IJ ， et al . S100A8/A9 drives monocytes towards M2-like macrophage differentiation and associates with M2-like macrophages in osteoarthritic synovium [J ] . Rheumatology （Oxford）， 2025 ， 64 （ 1 ）： 332 - 343 ．

Ren F ， Li J ， Yuan X ， et al . Dandelion polysaccharides exert anticancer effect on Hepatocellular carcinoma by inhibiting PI3K/AKT/mTOR pathway and enhancing immune response [J ] . J Funct Foods ， 2019 ， 55 ： 263 - 274 ．

Ren YS ， Zheng Y ， Duan H ， et al . Dandelion polyphenols protect against acetaminophen-induced hepatotoxicity in mice via activation of the Nrf-2/HO-1 pathwa y and inhibition of the JNK signaling pathway [J ] . Chin J Nat Med ， 2020 ， 18 （ 2 ）： 103 - 113 ．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect and mechanism of ginsenoside Rg1 in alleviating neuropathic pain in rats

Protective effect of biochanin A against ulcerative colitis in mice

Jiedu-Shengji ointment regulates GSK-3β/β-catenin to reduce inflammatory response to promote chronic wound healing in diabetic SD rats

Value of inflammatory，pyroptosis，and ferroptosis biomarkers in the treatment and prognosis of bladder cancer

Related Author

Yang Xiuhong

Li Han

Gao Xiaomeng

Dang Caiyan

Wang Kun

Guo Qiang

Hou Yi

Wang Zhuyun

Related Institution

Department of Pain，Xi’an Central Hospital

Laboratory of Traditional Chinese Medicine，Experimental Teaching and Management Center，Chongqing Medical University

Department of Obstetrics，The First Affiliated Hospital of Chongqing Medical University

The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases，the First Affiliated Hospital of Chongqing Medical University

School of Integrated Traditional Chinese and Western Medicine，Hunan University of Chinese Medicine

⁰