Effect of METTL3-mediated m<sup>6</sup>A modification of lncRNA SNHG5 on the proliferation and invasion of osteosarcoma MG-63 cells

Ruan Qiangjin; Gao Liang

doi:10.13406/j.cnki.cyxb.003859

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Effect of METTL3-mediated m⁶A modification of lncRNA SNHG5 on the proliferation and invasion of osteosarcoma MG-63 cells

Precision medicine in cancer and translational studies | 更新时间：2025-10-22

- Effect of METTL3-mediated m⁶A modification of lncRNA SNHG5 on the proliferation and invasion of osteosarcoma MG-63 cells
- Journal of Chongqing Medical University Vol. 50, Issue 8, Pages: 1053-1057(2025)
- 作者机构：
  
  1.云南省第三人民医院疼痛科，昆明 650011
  2.遵义医科大学附属第三人民医院创伤骨科，遵义　563000
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.003859
  CLC： R738
- Received：18 December 2023，
  
  Revised：2024-03-19，
  
  Accepted：28 March 2024，
  
  Published：28 August 2025
- 稿件说明：
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阮强津,高亮.METTL3介导m⁶A修饰lncRNA SNHG5对骨肉瘤MG-63细胞增殖和侵袭的影响[J].重庆医科大学学报,2025,50(8):1053-1057.

Ruan Qiangjin,Gao Liang.Effect of METTL3-mediated m⁶A modification of lncRNA SNHG5 on the proliferation and invasion of osteosarcoma MG-63 cells[J].Journal of Chongqing Medical University,2025,50(8):1053-1057.
阮强津,高亮.METTL3介导m⁶A修饰lncRNA SNHG5对骨肉瘤MG-63细胞增殖和侵袭的影响[J].重庆医科大学学报,2025,50(8):1053-1057. DOI： 10.13406/j.cnki.cyxb.003859.

Ruan Qiangjin,Gao Liang.Effect of METTL3-mediated m⁶A modification of lncRNA SNHG5 on the proliferation and invasion of osteosarcoma MG-63 cells[J].Journal of Chongqing Medical University,2025,50(8):1053-1057. DOI： 10.13406/j.cnki.cyxb.003859.

摘要

目的

探讨m

A甲基化转移酶3（methyltransferase 3，METTL3）在人骨肉瘤组织中的表达及其对MG-63细胞增殖和侵袭的影响。

方法

采用定量PCR和蛋白印迹检测METTL3在5对人骨肉瘤中的表达水平及其对下游lncRNA小核仁核糖核酸宿主基因5（small nucleolar RNA host gene 5，SNHG5）和Wnt-β-catenin信号通路的影响；甲基化RNA免疫共沉淀（methylated RNA immunoprecipitation，MeRIP）检测METTL3敲低对lncRNA SNHG5的m

A水平影响；CCK-8和Transwell实验检测细胞增殖和侵袭；蛋白印迹检测Wnt-β-catenin信号通路的活性变化。

结果

与癌旁正常组织相比，METTL3在人骨肉瘤组织中的mRNA（

=0.034）和蛋白水平（

=0.002）均可升高；敲低METTL3能减少lncRNA SNHG5的m

A水平（

=0.027）及其mRNA水平（

=0.002）。功能上，敲低METTL3抑制MG-63细胞的增殖（

=0.014）和侵袭（

=0.001），并抑制Wnt1，Wnt3a，Wnt10a，β-catenin和C-myc的蛋白表达水平（均

0.01），而SNHG5过表达促进细胞增殖（

=0.027）和侵袭（

=0.006），激活Wnt-β-catenin信号通路（均

0.01），并逆转METTL3敲低引起的抗肿瘤作用（均

0.01）。

结论

METTL3介导m

A修饰lncRNA SNHG5和激活Wnt-β-catenin信号促进骨肉瘤MG-63细胞增殖和侵袭的作用。

Abstract

Objective

To investigate the expression of m

A methyltransferase-like protein 3（METTL3） in human osteosarcoma（OS） tissue and the effect of METTL3 on the proliferation and invasion of MG-63 cells.

Methods

Quantitative PCR and Western blotting were used to measure the expression level of METTL3 in 5 pairs of human OS tissue samples and investigate the effect of METTL3 on the downstream long non-coding RNA（lncRNA） small nucleolar RNA host gene 5（SNHG5） and the Wnt/β-catenin signaling pathway；methylated RNA immunoprecipitation was used to observe the effect of METTL3 knockdown on the m

A level of lncRNA SNHG5；CCK-8 assay and Transwell assay were used to measure cell proliferation and invasion； Western blotting was used to measure the change in the activity of the Wnt/β-catenin signaling pathway.

Results

Compared with the paracancerous normal tissue，human OS tissue showed significant increases in the mRNA and protein expression levels of METTL3（

=0.034 and 0.002），and knockdown of METTL3 reduced the m

A level and mRNA expression level of lncRNA SNHG5（

=0.027 and 0.002）. Functionally，knockdown of METTL3 inhibited the proliferation and invasion of MG-63 cells（

=0.014 and 0.001） and the protein expression levels of Wnt1，Wnt3a，Wnt10a，β-catenin，and C-myc（all

0.01），while overexpression of SNHG5 promoted cell proliferation（

=0.027） and invasion（

=0.006），activated the Wnt/β-catenin signaling pathway（

0.01），and reversed the antitumor effect induced by METTL3 knockdown（

0.01）.

Conclusion

METTL3-mediated m

A modification of lncRNA SNHG5 promotes the proliferation and invasion of MG-63 cells by activating the Wnt/β-catenin signaling.

关键词

Keywords

references

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Related Institution

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⁰

Effect of METTL3-mediated m6A modification of lncRNA SNHG5 on the proliferation and invasion of osteosarcoma MG-63 cells

DOI：10.13406/j.cnki.cyxb.003859

摘要

Abstract

关键词

Keywords

references

Effect of METTL3-mediated m⁶A modification of lncRNA SNHG5 on the proliferation and invasion of osteosarcoma MG-63 cells