上海中医药大学附属第七人民医院重症医学科,上海 200137
Received:07 March 2025,
Revised:2025-04-08,
Accepted:07 April 2025,
Published:28 September 2025
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欧阳冰清,杨海南,齐璐瑶,等.二氧化铈纳米通过抗炎和抗氧化缓解急性胰腺炎[J].重庆医科大学学报,2025,50(9):1253-1260.
Ouyang Bingqing,Yang Hainan,Qi Luyao,et al.Cerium oxide nanoparticles alleviate acute pancreatitis through anti-inflammatory and antioxidant mechanisms[J].Journal of Chongqing Medical University,2025,50(9):1253-1260.
欧阳冰清,杨海南,齐璐瑶,等.二氧化铈纳米通过抗炎和抗氧化缓解急性胰腺炎[J].重庆医科大学学报,2025,50(9):1253-1260. DOI: 10.13406/j.cnki.cyxb.003858.
Ouyang Bingqing,Yang Hainan,Qi Luyao,et al.Cerium oxide nanoparticles alleviate acute pancreatitis through anti-inflammatory and antioxidant mechanisms[J].Journal of Chongqing Medical University,2025,50(9):1253-1260. DOI: 10.13406/j.cnki.cyxb.003858.
目的
2
探讨氧化铈纳米颗粒(cerium oxide nanoparticles,CeO₂ NPs)对急性胰腺炎(acute pancreatitis,AP)保护作用的机制,聚焦在其抗氧化和抗炎特性。
方法
2
使用透射电子显微镜(transmission electron microscope,TEM)和动态光散射(dynamic light scattering,DLS)对CeO₂ NPs进行表征验证。体外实验运用细胞增殖/毒性检测试剂盒(cell counting Kit-8,CCK-8)、流式细胞术和蛋白质免疫印迹法验证CeO₂ NPs防止胰腺腺泡细胞凋亡的作用。体内实验将C57BL/6小鼠分为对照组、AP组、AP+CeO₂组、SAP组和SAP+CeO₂组,探究CeO₂ NPs缓解AP小鼠炎症和氧化应激的作用机制。
结果
2
CeO₂ NPs具有较好的稳定性和生物相容性,TEM显示其粒径为(50±4) nm。体外实验结果显示,CeO₂ NPs通过减轻脂质过氧化和维持线粒体膜电位,明显减少腺泡细胞凋亡。体内实验结果显示,CeO₂ NPs降低血清淀粉酶、脂肪酶和炎症细胞因子(白细胞介素-6、肿瘤坏死因子-α)含量。这一结果可能与IKK/P53/bcl-2通路的调控相关。CeO₂ NPs减少活性氧产生并通过调节Nrf-2信号通路增强了抗氧化反应。
结论
2
CeO₂ NPs通过调控IκB激酶/肿瘤蛋白p53/B细胞淋巴瘤-2(IκB kinase/tumor protein p53/B-cell lymphoma 2,IKK/P53/bcl-2)和核因子E2相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)信号通路发挥抗炎和抗氧化作用,对AP具有良好的治疗潜力。
Objective
2
To investigate the protective mechanism of cerium oxide nanoparticles(CeO
2
NPs) against acute pancreatitis (AP),with a focus on their antioxidant and anti-inflammatory properties.
Methods
2
CeO
2
NPs were characterized by transmission electron microscopy(TEM) and dynamic light scattering. In
in vitro
experiments,cell counting Kit-8(CCK-8) assay,flow cytometry,and Western blotting were used to validate the role of CeO
2
NPs in preventing the apoptosis of pancreatic acinar cells. In
in vivo
experiments,C57BL/6 mice were divided into control group,AP group,AP+CeO
2
group,SAP group,and SAP+CeO
2
group to investigate the mechanism of action of CeO
2
NPs in alleviating inflammation and oxidative stress in AP mice.
Results
2
CeO
2
NPs demonstrated relatively good stability and biocompatibi
lity,with a particle size of (50±4) nm on TEM.
In vitro
experiments showed that CeO
2
NPs significantly reduced the apoptosis of pancreatic acinar cells by alleviating lipid peroxidation and maintaining mitochondrial membrane potential.
In vivo
experiments showed that CeO
2
NPs could reduce the serum levels of amylase,lipase,and inflammatory cytokines (interleukin-6 and tumor necrosis factor-α). This result might be related to the regulation of the IKK/P53/Bcl-2 pathway. CeO
2
NPs reduced the production of reactive oxygen species and enhanced antioxidant response by regulating the Nrf-2 signaling pathway.
Conclusion
2
CeO
2
NPs exert anti-inflammatory and antioxidant effects by regulating the IκB kinase/tumor protein p53/B-cell lymphoma 2(IKK/P53/bcl-2) and nuclear factor erythroid 2-related(Nrf-2) signaling pathways,thereby showing promising potential for the treatment of AP.
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