Activation of glial cell pyroptosis signal in children with sepsis and its influence on mitochondrial injury in neural cells

Xing Jing; Wang Yanfei; Yan Huijuan; Wang Xinmei; Liu Zhenkui

doi:10.13406/j.cnki.cyxb.003832

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Activation of glial cell pyroptosis signal in children with sepsis and its influence on mitochondrial injury in neural cells

Excellence in medical insights\：pathogenesis and clinical treatment of sepsis | 更新时间：2026-06-11

- Activation of glial cell pyroptosis signal in children with sepsis and its influence on mitochondrial injury in neural cells
- Journal of Chongqing Medical University Vol. 51, Issue 6, Pages: 829-837(2026)
- 作者机构：
  
  河北北方学院附属第一医院儿内科，张家口 075000
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.003832
  CLC： R459.7
- Received：18 December 2024，
  
  Revised：2025-03-04，
  
  Published：28 June 2026
- 稿件说明：
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邢静,王艳飞,闫慧娟等.儿童脓毒症胶质细胞焦亡信号活化及其对神经细胞线粒体损伤的影响力[J].重庆医科大学学报,2026,51(06):829-837.

Xing Jing,Wang Yanfei,Yan Huijuan,et al.Activation of glial cell pyroptosis signal in children with sepsis and its influence on mitochondrial injury in neural cells[J].Journal of Chongqing Medical University,2026,51(06):829-837.
邢静,王艳飞,闫慧娟等.儿童脓毒症胶质细胞焦亡信号活化及其对神经细胞线粒体损伤的影响力[J].重庆医科大学学报,2026,51(06):829-837. DOI： 10.13406/j.cnki.cyxb.003832.

Xing Jing,Wang Yanfei,Yan Huijuan,et al.Activation of glial cell pyroptosis signal in children with sepsis and its influence on mitochondrial injury in neural cells[J].Journal of Chongqing Medical University,2026,51(06):829-837. DOI： 10.13406/j.cnki.cyxb.003832.

摘要

目的

探讨儿童脓毒症相关脑病（sepsis-associated encephalopathy，SAE）胶质细胞焦亡信号活化及其对神经细胞线粒体损伤的影响力。

方法

纳入河北北方学院附属第一医院2020年4月至2024年3月儿童重症监护病房（pediatric intensive care unit，PICU）26例脓毒症患者，包括15例SAE和11例非SAE。采用实时荧光定量聚合酶链式反应（real-time quantitative polymerase chain reaction，qPCR）定量血清中线粒体DNA（mitochondrial DNA，mtDNA），酶联免疫吸附法检测血清中S100钙结合蛋白B（S100 calcium-binding protein B，S100B）、神经特异性烯醇化酶（neuron-specific enolase，NSE）水平。将BV2细胞分为Con组、mtDNA组、si-cGAS组、mtDNA+si-cGAS组、mtDNA+cGAS-oe组和mtDNA+cGAS-oe+YVAD-cmk组。采用蛋白质印迹检测核因子κB/NOD 样受体热蛋白结构域蛋白3（nuclear factor kappa-B/NOD-like receptor pyrin domain containing 3，NF-κB/NLRP3）信号，流式细胞术检测细胞焦亡。

结果

SAE组在住院第4、7天的血清mtDNA水平明显高于非SAE组（

0.05）。血清mtDNA水平与NSE、S100B水平呈正相关（

0.05）。转染si-cGAS明显逆转了mtDNA对BV2细胞NF-κB/NLRP3信号激活和细胞焦亡的影响（

0.05）。与mtDNA组相比，mtDNA+cGAS-oe组BV2细胞中cGAS、p-NF-κB、NLRP3、Cleaved caspase-1蛋白表达和细胞焦亡率明显增加（

0.05）。与mtDNA+cGAS-oe

组相比，mtDNA+cGAS-oe+YVAD-cmk组BV2细胞中Cleaved caspase-1蛋白表达和细胞焦亡率明显降低（

0.05）。

结论

脓毒症患儿血清mtDNA水平与NSE、S100B水平变化密切相关，其可能以cGAS依赖方式促进BV2细胞NF-κB/NLRP3信号激活和细胞焦亡。

Abstract

Objective

To investigate the activation of glial cell pyroptosis signal in children with sepsis-related encephalopathy （SAE） and its influence on mitochondrial injury in neural cells.

Methods

A total of 26 patients with sepsis who were admitted to

the First Affiliated Hospital of Hebei North University from April 2020 to March 2024 were enrolled，among whom there were 15 SAE patients and 11 non-SAE patients. Quantitative PCR was used to measure mitochondrial DNA（mtDNA） in serum，and ELISA was used to measure the serum levels of S100 calcium-binding protein B（S100B） and neuron-specific enolase（NSE）. BV2 cells were divided into Con group，mtDNA group，si-cGAS group，mtDNA+si-cGAS group，mtDNA+cGAS-oe group，and mtDNA+cGAS-oe+YVAD-cmk group. Western blotting was used to analyze nuclear factor kappa-B/NOD-like receptor pyrin domain containing 3（NF-κB/NLRP3） signal，and flow cytometry was used to measure cell pyroptosis.

Results

The SAE group had a significantly higher serum level of mtDNA than the non-SAE group in days 4 and 7 of hospitalization（

0.05）. The serum level of mtDNA was positively correlated with the serum levels of NSE and S100B（

0.05）. Transfection of si-cGAS significantly reversed the effect of mtDNA on NF-κB/NLRP3 signal activation and cell pyroptosis in BV2 cells（

0.05）. Compared with the mtDNA group，the mtDNA+cGAS-oe group had significant increases in the protein expression levels of cGAS，p-NF-κB，NLRP3，and cleaved caspase-1 and cell pyroptosis rate in BV cells（

0.05）. Compared with the mtDNA+cGAS-oe group，the mtDNA+cGAS-oe+YVAD-cmk group had significan

t reductions in the protein expression level of cleaved caspase-1 and cell pyroptosis rate in BV2 cells（

0.05）.

Conclusion

The serum level of mtDNA in children with sepsis is closely associated with the changes in the serum levels of NSE and S100B，and the increase in mtDNA may promote the activation of NF-κB/NLRP3 signal and cell apoptosis in BV2 cells in a cGAS-dependent manner.

关键词

Keywords

references

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