Effect of hematopoietic stem cell transplantation therapy on lymphocyte subsets and blood cell counts in patients with aplastic anemia

Cui Zhaoting; Chen Shuaiyin; Duan Guangcai

doi:10.13406/j.cnki.cyxb.003808

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Effect of hematopoietic stem cell transplantation therapy on lymphocyte subsets and blood cell counts in patients with aplastic anemia

Clinical research | 更新时间：2026-02-13

- Effect of hematopoietic stem cell transplantation therapy on lymphocyte subsets and blood cell counts in patients with aplastic anemia
- Journal of Chongqing Medical University Vol. 51, Issue 1, Pages: 146-150(2026)
- 作者机构：
  
  1.郑州市第三人民医院中心实验室，郑州 450001
  2.郑州大学公共卫生学院流行病与卫生统计学系，郑州 450001
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.003808
  CLC： R473.72
- Received：15 July 2024，
  
  Revised：2025-01-01，
  
  Published：28 January 2026
- 稿件说明：
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崔昭婷,陈帅印,段广才.造血干细胞移植治疗对再生障碍性贫血患者淋巴细胞亚群及血细胞计数的影响[J].重庆医科大学学报,2026,51(01):146-150.

Cui Zhaoting,Chen Shuaiyin,Duan Guangcai.Effect of hematopoietic stem cell transplantation therapy on lymphocyte subsets and blood cell counts in patients with aplastic anemia[J].Journal of Chongqing Medical University,2026,51(01):146-150.
崔昭婷,陈帅印,段广才.造血干细胞移植治疗对再生障碍性贫血患者淋巴细胞亚群及血细胞计数的影响[J].重庆医科大学学报,2026,51(01):146-150. DOI： 10.13406/j.cnki.cyxb.003808.

Cui Zhaoting,Chen Shuaiyin,Duan Guangcai.Effect of hematopoietic stem cell transplantation therapy on lymphocyte subsets and blood cell counts in patients with aplastic anemia[J].Journal of Chongqing Medical University,2026,51(01):146-150. DOI： 10.13406/j.cnki.cyxb.003808.

摘要

目的

分析研究造血干细胞移植治疗对再生障碍性贫血患者淋巴细胞亚群及血细胞计数的影响。

方法

选取2019年2月至2022年4月通过造血干细胞移植治疗的获得性再生障碍性贫血患者84例。分别检测治疗前、治疗后1个月、治疗后3个月、治疗后6个月外周血淋巴细胞亚群以及血细胞变化，包括CD19

B细胞、CD3

T细胞、CD3

CD4

辅助T细胞、CD3

CD8

抑制T细胞、CD3

CD16

CD56

NK细胞、CD3

CD16

CD56

NKT细胞百分比、CD4

/CD8

T细胞比值、淋巴细胞占白细胞百分比、白细胞计数、红细胞计数及血小板计数等11项。

结果

患者治疗前外周血CD3

、CD3

CD8

、CD3

CD16

CD56

、淋巴细胞占白细胞百分比均高于正常值，CD4/CD8、白细胞计数、红细胞计数、血小板计数均低于正常值。治疗前后CD19

、CD3

CD4

、CD3

CD16

CD56

均维持正常水平。CD4

/CD8

、血小板计数在治疗后6个月内仍低于正常值。其余均在3个月左右恢复至正常水平。

结论

再生障碍性贫血可以导致患者免疫系统存在不同程度的紊乱，且全身血细胞减少。造血干细胞移植可以较好的治疗再生障碍性贫血，患者淋巴细胞亚群以及血细胞在3个月左右恢复正常水平。

Abstract

Objective

To investigate the effect of hematopoietic stem cell transplantation therapy on lymphocyte subsets and blood cell counts in patients with aplastic anemia.

Methods

A total of 84 patients with acquired aplastic anemia who received hematopoietic stem cell transplantation from February 2019 to April 2022 were enrolled. Peripheral blood lymphocyte subsets and blood cell counts were measured before treatment and at 1，3，and 6 months after treatment，including the percentages of CD19+ B cells，CD3

T cells，CD3

CD4

helper T cells，CD3

CD8

suppressor T cells，CD3

CD16

CD56

NK cells，and CD3

CD16

CD56

NKT cells，as well as CD4

/CD8

T cell ratio，the percentage of lymphocytes in white blood cells，white blood cell count，red blood cell count，and platelet count.

Results

Before treatment，the percentages of peripheral blood CD3

，CD3

CD8

，and CD3

CD16

CD56

cells and the percentage of lymphocytes in white blood cells were all above the normal values，while CD4/CD8 ratio，white blood cell count，red blood cell count，and platelet count were all below the normal values. CD19

，CD3

CD4

，and CD3

CD16

CD56

were maintained at normal levels before and after treatment. CD4

/CD8

ratio and platelet count remained below the normal values within 6 months after treatment，while the other values returned to normal within approximately 3 months.

Conclusion

Aplastic anemia can lead to varying degrees of immune system disorders and a reduction in systemic blood cells. Hematopoietic stem cell transplantation can be a better treatment method for aplastic anemia，with lymphocyte subsets and blood cell counts returning to normal within about 3 months.

关键词

Keywords

references

Brzeźniakiewicz-Janus K ， Rupa-Matysek J ， Gil L . Acquired aplastic Anemia as a clonal disorder of hematopoietic stem cells [J ] . Stem Cell Rev Rep ， 2020 ， 16 （ 3 ）： 472 - 481 ．

Kulasekararaj A ， Cavenagh J ， Dokal I ， et al . Guidelines for the diagnosis and management of adult aplastic anaemia：a British Society for Haematology Guideline [J ] . Br J Haematol ， 2024 ， 204 （ 3 ）： 784 - 804 ．

Yu W ， Wang QQ ， Ge ML ， et al . Cluster analysis of lymphocyte subset from peripheral blood in newly diagnosed idiopathic aplastic anaemia patients [J ] . Ann Med ， 2022 ， 54 （ 1 ）： 2431 - 2439 ．

Zhang Y ， Zhang Q ， Ha XQ ， et al . Research progress in treatment of aplastic anemia [J ] . Journal of Clinical Medicine in Practice ， 2023 ， 27 （ 23 ）: 144 - 148 .

Zhang YF ， Huo JL ， Liu L ， et al . Comparison of hematopoietic stem cell transplantation outcomes using matched sibling donors，haploidentical donors，and immunosuppressive therapy for patients with acquired aplastic Anemia [J ] . Front Immunol ， 2022 ， 13 ： 837335 ．

Gennery AR . Hematopoietic stem cell transplantation for primary immune deficiencies [M ] // Stiehm’s Immune Deficiencies . Amsterdam ： Elsevier ， 2020 ： 1175 - 1214 ．

Wang L ， Liu H . Pathogenesis of aplastic anemia [J ] . Hematology ， 2019 ， 24 （ 1 ）： 559 - 566 ．

Malard F ， Holler E ， Sandmaier BM ， et al . Acute graft-versus-host disease [J ] . Nat Rev Dis Primers ， 2023 ， 9 （ 1 ）： 27 ．

Hematopoietic Stem Cell Application Group CSOH ， China Association for the Prevention of Hematology Diseases . Chinese consensus on the diagnosis and management of chronic graft-versus-host disease （2021） [J ] . Zhonghua Xue Ye Xue Za Zhi ， 2021 ， 42 （ 4 ）： 265 - 275 ．

Giudice V ， Selleri C . Aplastic anemia：pathophysiology [J ] . Semin Hematol ， 2022 ， 59 （ 1 ）： 13 - 20 ．

Jacobson LO ， Simmons EL ， Marks EK ， et al . Recovery from radiation injury [J ] . Science ， 1951 ， 113 （ 2940 ）： 510 - 511 ．

Bhatt ST ， Bednarski JJ . Immune reconstitution in pediatric patients following hematopoietic cell transplant for non-malignant disorders [J ] . Front Immunol ， 2020 ， 11 ： 1988 ．

Patel BA ， Giudice V ， Young NS . Immunologic effects on the haematopoietic stem cell in marrow failure [J ] . Best Pract Res Clin Haematol ， 2021 ， 34 （ 2 ）： 101276 ．

Belinovski AR ， Pelegrina PD ， Lima ACM ， et al . Immune reconstitution after allogenic stem cell transplantation：an observational study in pediatric patients [J ] . Hematol Transfus Cell Ther ， 2023 ， 45 （ 2 ）： 235 - 244 .

Subedi N ， Verhagen LP ， Bosman EM ， et al . Understanding natural killer cell biology from a single cell perspective [J ] . Cell Immunol ， 2022 ， 373 ： 104497 ．

Quatrini L ， Della Chiesa M ， Sivori S ， et al . Human NK cells，their receptors and function [J ] . Eur J Immunol ， 2021 ， 51 （ 7 ）： 1566 - 1579 ．

Li Y ， Ding SX ， Liu CY ， et al . Abnormalities of quantities and functions of CD56bright natural killer cells in non-severe aplastic Anemia [J ] . Hematology ， 2019 ， 24 （ 1 ）： 405 - 412 ．

Tjon JM ， Langemeijer SMC ， Halkes CJM . Anti thymocyte globulin-based treatment for acquired bone marrow failure in adults [J ] . Cells ， 2021 ， 10 （ 11 ）： 2905 ．

Wang JY ， Yuan M ， Zhu GH ， et al . Immune reconstitution in pediatric aplastic Anemia after allogeneic hematopoietic stem-cell transplantation [J ] . Int J Med Sci ， 2022 ， 19 （ 5 ）： 821 - 828 ．

DeZern AE ， Churpek JE . Approach to the diagnosis of aplastic anemia [J ] . Blood Adv ， 2021 ， 5 （ 12 ）： 2660 - 2671 ．

Bento L ， Canaro M ， Bastida JM ， et al . Thrombocytopenia and therapeutic strategies after allogeneic hematopoietic stem cell transplantation [J ] . J Clin Med ， 2022 ， 11 （ 5 ）： 1364 ．

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