Effect of bone marrow mesenchymal stem cells in repairing IFN-γ-induced ovarian granulosa cell immune injury

Nie Jia; Ma Wenqing; Zhuo Aiping; Yang Mingxin; Mao Mengli; Fu Xiafei

doi:10.13406/j.cnki.cyxb.003775

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Effect of bone marrow mesenchymal stem cells in repairing IFN-γ-induced ovarian granulosa cell immune injury

Basic research | 更新时间：2025-11-20

- Effect of bone marrow mesenchymal stem cells in repairing IFN-γ-induced ovarian granulosa cell immune injury
- Journal of Chongqing Medical University Vol. 50, Issue 11, Pages: 1525-1530(2025)
- 作者机构：
  
  1.南方医科大学珠江医院妇产科，广州 510220
  2.苏州大学附属第一医院妇产科，苏州 215006
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.003775
  CLC： R711.75
- Received：04 August 2024，
  
  Revised：2024-12-03，
  
  Accepted：16 December 2024，
  
  Published：28 November 2025
- 稿件说明：
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聂佳,马闻擎,卓爱萍等.骨髓间充质干细胞修复IFN-γ诱导的卵巢颗粒细胞免疫损伤的研究[J].重庆医科大学学报,2025,50(11):1525-1530.

Nie Jia,Ma Wenqing,Zhuo Aiping,et al.Effect of bone marrow mesenchymal stem cells in repairing IFN-γ-induced ovarian granulosa cell immune injury[J].Journal of Chongqing Medical University,2025,50(11):1525-1530.
聂佳,马闻擎,卓爱萍等.骨髓间充质干细胞修复IFN-γ诱导的卵巢颗粒细胞免疫损伤的研究[J].重庆医科大学学报,2025,50(11):1525-1530. DOI： 10.13406/j.cnki.cyxb.003775.

Nie Jia,Ma Wenqing,Zhuo Aiping,et al.Effect of bone marrow mesenchymal stem cells in repairing IFN-γ-induced ovarian granulosa cell immune injury[J].Journal of Chongqing Medical University,2025,50(11):1525-1530. DOI： 10.13406/j.cnki.cyxb.003775.

摘要

目的

探讨骨髓间充质干细胞对干扰素-γ（interferon gamma，IFN-γ）诱导的卵巢颗粒细胞免疫损伤的修复作用。

方法

构建IFN-γ诱导的卵巢颗粒细胞免疫损伤模型，将建模后的人卵巢颗粒细胞（human ovary granulosa cell line，KGN）与人骨髓间充质干细胞（human bone mesenchymal stem cells，hBMSC）共培养，分为3组：NC组，IFN-γ组和BMSC组。共培养结束后连续进行3 d的CCK-8实验检测细胞增殖情况，使用Annexin V-FITC和CytoFLEX流式细胞仪检测细胞凋亡情况，检测雌二醇水平和芳香化酶细胞色素P450家族19亚家族A成员1（cytochrome P450 family 19 subfamily A member 1，CYP19A1）、卵泡刺激素受体（follicle-stimulating hormone receptor，FSH receptor，FSHR） mRNA表达水平以评估激素合成能力，检测乳酸脱氢酶（lactate dehydrogenase，LDH）水平，NOD样受体热蛋白结构域相关蛋白3（NOD-like receptor thermal protein domain associated protein 3，NLRP3）、半胱天冬酶1（caspase-1，CASP1）和白细胞介素-1β（interleukin-1β，IL-1β）的mRNA表达及NLRP3、CASP1、焦孔素（gasdermin-D，GSDMD）的蛋白表达水平以评估细胞焦亡。

结果

IFN-γ组和BMSC组24 h、48 h、72 h的颗粒细胞增殖率均低于NC组，且IFN-γ组低于BMSC组（

0.001）。INF-γ组和BMSC组颗粒细胞凋亡数量高于NC组，且INF-γ组高于BMSC组（

0.001）。INF-γ组和BMSC组雌二醇水平低于NC组，且INF-γ组低于BMSC组（

0.001）。与IFN-γ组相比，BMSC组的CYP19A1及FSHR mRNA的表达下降（

0.001）。与IFN-γ组相比，BMSC组LDH水平降低，NLRP3和CASP1的mRNA表达水平降低，caspase-1的蛋白表达水平降低，差异均有统计学意义。

结论

hBMSC对IFN-γ诱导的KGN细胞免疫损伤有修复作用，具有恢复细胞增殖、抑制细胞凋亡、修复内分泌功能和减少细胞焦亡的作用。

Abstract

Objective

To investigate the repair effect of bone marrow mesenchymal stem cells（BMSCs） on interferon gamma（IFN-γ） induced ovarian granulosa cell immune injury.

Methods

A model of IFN-γ-induced ovarian granulosa cell immune injury was established. KGN cells after modeling were co-cultured with human BMSCs（hBMSCs） and divided into three groups：negative control（NC） group，IFN-γ group，and BMSC group. After co-culture，cell proliferation was determined by a cell counting kit 8 assay for 3 consecutive days. Cell apoptosis was determined using an Annexin V-FITC apoptosis detection kit and a CytoFLEX flow cytometer. The estradiol level and the mRNA expression levels of aromatase CYP19A1 and FSHR were measured to evaluate the hormone synthesis ability. The level of lactate dehydrogenase（LDH），mRNA expression levels of NLR

P3，caspase-1（CASP1），and interleukin-1β，and protein expression levels of NLRP3，CASP1，and gasdermin-D were determined to evaluate cell pyroptosis

Results

The proliferation rate of granulosa cells in the IFN-γ group and the BMSC group was lower than that in the NC group at 24 h，48 h and 72 h，and that in the IFN-γ group was lower than that in the BMSC group（

0.001）. The number of apoptotic granulosa cells in the INF-γ group and the BMSC group was higher than that in the NC group，and that in the INF-γ group was higher than that in the BMSC group（

0.001）. The estradiol level in the INF-γ group and the BMSC group was lower than that in the NC group，and that in the INF-γ group was lower than that in the BMSC group（

0.001）. Compared with the IFN-γ group，the mRNA expression of CYP19A1 and FSHR in the BMSC group decreased（

0.001）. Compared with the IFN-γ group，the BMSC group had significantly decreased LDH level，mRNA expression levels of NLRP3 and CASP1，and protein expression level of CASP1.

Conclusion

hBMSCs repair the immune injury of KGN cells induced by IFN-γ by restoring cell proliferation，inhibiting cell apoptosis，repairing endocrine function，and reducing pyroptosis.

关键词

Keywords

references

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