Mechanism of FERMT1 promoting lung adenocarcinoma metastasis by regulating epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway

Wu Qifei; Li Xiaotian; He Huijie; Niu Haiying; Liu Jingyi; Zhang Dong

doi:10.13406/j.cnki.cyxb.003770

您当前的位置：

首页 >

文章列表页 >

Mechanism of FERMT1 promoting lung adenocarcinoma metastasis by regulating epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway

Precision medicine in cancer and translational studies | 更新时间：2025-12-31

- Mechanism of FERMT1 promoting lung adenocarcinoma metastasis by regulating epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway
- Journal of Chongqing Medical University Vol. 50, Issue 12, Pages: 1673-1682(2025)
- 作者机构：
  
  内蒙古科技大学包头医学院第一附属医院呼吸与危重症科，包头 014000
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.003770
  CLC： R734.2
- Received：24 April 2024，
  
  Revised：2024-11-07，
  
  Accepted：24 November 2024，
  
  Published：28 December 2025
- 稿件说明：
移动端阅览
吴启飞,李晓甜,何慧洁等.FERMT1通过Wnt/β-catenin信号通路调控EMT促进肺腺癌转移的机制研究[J].重庆医科大学学报,2025,50(12):1673-1682.

Wu Qifei,Li Xiaotian,He Huijie,et al.Mechanism of FERMT1 promoting lung adenocarcinoma metastasis by regulating epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway[J].Journal of Chongqing Medical University,2025,50(12):1673-1682.
吴启飞,李晓甜,何慧洁等.FERMT1通过Wnt/β-catenin信号通路调控EMT促进肺腺癌转移的机制研究[J].重庆医科大学学报,2025,50(12):1673-1682. DOI： 10.13406/j.cnki.cyxb.003770.

Wu Qifei,Li Xiaotian,He Huijie,et al.Mechanism of FERMT1 promoting lung adenocarcinoma metastasis by regulating epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway[J].Journal of Chongqing Medical University,2025,50(12):1673-1682. DOI： 10.13406/j.cnki.cyxb.003770.

摘要

目的

探讨FERMT1在肺腺癌细胞中的表达情况，研究FERMT1调控Wnt信号通对肺腺癌细胞增殖、侵袭、迁移、凋亡的影响及其机制。

方法

本研究利用生物信息学分析了FERMT1在肺腺癌中的表达及其与患者临床特征和预后的关系；并采用体外实验研究FERMT1对肺腺癌生物学行为的作用，使用实时荧光定量聚合酶链反应（quantitative real-time polymerase chain reaction，qRT-PCR）和蛋白质印迹技术（Western blot）方法检测分析FERMT1表达水平的变化对Wnt/β连环蛋白（β-catenin）通路及其下游靶基因的影响。

结果

生物信息学分析显示FERMT1在肺腺癌组织中高表达，肺腺癌TNM分期中Ⅰ~ Ⅳ期FERMT1的表达水平均高于正常肺组织，同时，FERMT1的高表达导致肺腺癌患者的不良预后，Wnt信号通路是其主要通路。体外实验显示，抑制FERMT1的表达降低肺腺癌细胞的增殖、迁移和侵袭能力，增加肺腺癌细胞的凋亡。qRT-PCR和Western blot结果显示，FERMT1表达的变化影响β-catenin、上皮间质转化（epithelial-mesenchymal transition，EMT）相关标志物及其下游靶基因的表达。

结论

FERMT1与肺腺癌发生发展密切相关，Wnt/β-catenin信号通路是其作用的机制之一。

Abstract

Objective

To investigate the expression of FERMT1 in lung adenocarcinoma cells and the effect and mechanism of FERMT1 on the proliferation，invasion，migration，and apoptosis of lung adenocarcinoma cells by regulating the Wnt signaling pathway.

Methods

The bioinformatics analysis was used to investigate the expression of FERMT1 in lung adenocarcinoma and its association with clinical features and prognosis；

in vitro

experiments were used to investigate the influence of FERMT1 on the biological behavior of lung adenocarcinoma；qRT-PCR and Western blot were used to observe the effect of the change in FERMT1 expression on the Wnt/β-catenin pathway and its downstream target genes.

Results

The bioinformatics analysis showed the high expression of FERMT1 in lung adenocarcinoma tissue，the expression level of FERMT1 in TNM staging of lung adenocarcinoma was higher than that in normal lung tissue，and meanwhile，the high expression of FERMT1 led to the poor prognosis of lung adenocarcinoma patients，mainly through the Wnt signaling pathway.

In vitro

experiments showed that inhibition of FERMT1 expression reduced the proliferation，migration，and invasion abilities of lung adenocarcinoma cells and increased the apoptosis of lung adenocarcinoma cells. The results of qRT-PCR and Western blot showed that the change in FERMT1 expression affected the expression of β-catenin，epithelial-mesenchymal transition-related markers，and their downstream target g

enes.

Conclusion

FERMT1 is closely associated with the development and progression of lung adenocarcinoma through the Wnt/β-catenin signaling pathway.

关键词

Keywords

references

Chen PX ， Liu YH ， Wen YK ， et al . Non-small cell lung cancer in China [J ] . Cancer Commun ， 2022 ， 42 （ 10 ）： 937 - 970 ．

Xiao YX ， Liu P ， Wei J ， et al . Recent progress in targeted therapy for non-small cell lung cancer [J ] . Front Pharmacol ， 2023 ， 14 ： 1125547 ．

Emmert H ， Patel H ， Brunton VG . Kindlin-1 protects cells from oxidative damage through activation of ERK signalling [J ] . Free Radic Biol Med ， 2017 ， 108 ： 896 - 903 ．

Liu CC ， Cai DL ， Sun F ， et al . FERMT1 mediates epithelial-mesenchymal transition to promote colon cancer metastasis via modulation of β-catenin transcriptional activity [J ] . Oncogene ， 2017 ， 36 （ 13 ）： 1779 - 1792 ．

Fan H ， Zhang SJ ， Zhang Y ， et al . FERMT1 promotes gastric cancer progression by activating the NF-κB pathway and predicts poor prognosis [J ] . Cancer Biol Ther ， 2020 ， 21 （ 9 ）： 815 - 825 ．

Chen YL ， Wang K ， Xie F ， et al . Novel biomarkers identified in triple-negative breast cancer through RNA-sequencing [J ] . Clin Chim Acta ， 2022 ， 531 ： 302 - 308 ．

Wang X ， Chen QQ . FERMT1 knockdown inhibits oral squamous cell carcinoma cell epithelial-mesenchymal transition by inactivating the PI3K/AKT signaling pathway [J ] . BMC Oral Health ， 2021 ， 21 （ 1 ）： 598 ．

Sun RZ ， Hou ZS ， Zhang YK ， et al . Drug resistance mechanisms and progress in the treatment of EGFR-mutated lung adenocarcinoma [J ] . Oncol Lett ， 2022 ， 24 （ 5 ）： 408 ．

Rognoni E ， Ruppert R ， Fässler R . The kindlin family：functions，signaling properties and implications for human disease [J ] . J Cell Sci ， 2016 ， 129 （ 1 ）： 17 - 27 ．

Purushothaman A ， Mohajeri M ， Lele TP . The role of glycans in the mechanobiology of cancer [J ] . J Biol Chem ， 2023 ， 299 （ 3 ）： 102935 ．

Uesaka K ， Oka H ， Kato R ， et al . Bioinformatics in bioscience and bioengineering：recent advances，applications，and perspectives [J ] . J Biosci Bioeng ， 2022 ， 134 （ 5 ）： 363 - 373 ．

Su XS ， Wu WJ ， Lin XP ， et al . Research progress on the Kindlin family's regulation of the Wnt/β-catenin signaling pathway [J ] . Life Sciences . 2020 ； 32 （ 5 ）： 424 - 430 ．

Jiang JW ， Li JY ， Zhou XM ， et al . Exosomes regulate the epithelial-mesenchymal transition in cancer [J ] . Front Oncol ， 2022 ， 12 ： 864980 ．

Mirzaei S ， Saghari S ， Bassiri F ， et al . NF-κB as a regulator of cancer metastasis and therapy response：a focus on epithelial-mesenchymal transition [J ] . J Cell Physiol ， 2022 ， 237 （ 7 ）： 2770 - 2795 ．

Li JX ， Xie GY ， Tian YF ， et al . RNA m 6 A methylation regulates dissemination of cancer cells by modulating expression and membrane localization of β-catenin [J ] . Mol Ther ， 2022 ， 30 （ 4 ）： 1578 - 1596 ．

Li ZQ ， Wu SW ， Liu W . Advances of Wnt/β-catenin signaling pathway in lung cancer：a review [J ] . Altern Ther Health Med ， 2024 ， 30 （ 1 ）： 238 - 247 ．

Pan JC ， Fang S ， Tian HH ， et al . lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/β-catenin signaling [J ] . Mol Cancer ， 2020 ， 19 （ 1 ）： 9 ．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Value of multi-planar volume reconstructions in evaluating the aggressiveness of lung ground glass nodules

Identification and validation of circulating exosome-derived microRNAs for the early diagnosis of gastric cancer

Screening for feature genes and immune infiltration of multiple myeloma: a study based on support vector machine

Anti-tumor effect and prognostic value of ubiquitin-specific protease 53 in colorectal cancer

Establishment and preliminary analysis of lung adenocarcinoma cell lines with stable MINK1 knockdown

Related Author

Zhang Ke

Jing Weiwei

Li Dan

Luo Renjun

Tong Liang

Zhang Yundan

Lü Fajin

Yang Liu

Related Institution

Department of Pathology，The First Affiliated Hospital of Chongqing Medical University

Department of Radiology，The First Affiliated Hospital of Chongqing Medical University

Key Laboratory of Ministry of Education for Biorheological Science and Technology，School of Bioengineering，Chongqing University

Department of Laboratory Medicine，The Third Affiliated Hospital of Chongqing Medical University

Department of Hematology，The First Affiliated Hospital of Chongqing Medical University

⁰