Study on the difference of bone parameters and micromechanical parameters of ribs between human and non-human species
- Journal of Chongqing Medical University Vol. 49, Issue 12, Pages: 1533-1540(2024)
- 作者机构：
1.贵州医科大学法医学院，贵阳 550004
2.大连大学附属中山医院骨科，大连 116001
3.贵州医科大学基础医学院人体解剖学教学实验中心，贵阳 550004
4.贵州中医药大学大学城医院，贵阳 550004
5.遵义医科大学医学与科技学院，遵义 564600
- 作者简介：
- 基金信息：
DOI：10.13406/j.cnki.cyxb.003670
CLC： R89Received：24 June 2024，
Revised：2024-10-06，
Accepted：28 October 2024，
Published：28 December 2024
- 稿件说明：
移动端阅览
吴文鑫,邱兴,夏冰,等.人类与非人类物种肋骨骨质参数与微观力学参数差异研究[J].重庆医科大学学报,2024,49(12):1533-1540.
Wu Wenxin,Qiu Xing,Xia Bing,et al.Study on the difference of bone parameters and micromechanical parameters of ribs between human and non-human species[J].Journal of Chongqing Medical University,2024,49(12):1533-1540.
吴文鑫,邱兴,夏冰,等.人类与非人类物种肋骨骨质参数与微观力学参数差异研究[J].重庆医科大学学报,2024,49(12):1533-1540. DOI： 10.13406/j.cnki.cyxb.003670.
Wu Wenxin,Qiu Xing,Xia Bing,et al.Study on the difference of bone parameters and micromechanical parameters of ribs between human and non-human species[J].Journal of Chongqing Medical University,2024,49(12):1533-1540. DOI： 10.13406/j.cnki.cyxb.003670.
摘要
目的
2
检测不同物种肋骨骨质参数与微观力学参数并分析其差异，探索利用肋骨上述参数鉴别不同物种的可行性。
方法
2
提取人、猪、牛、羊、猴的左侧第四肋骨起始于肋软骨外约1 cm、长1 cm肋骨，利用Micro-CT检测上述物种肋骨的皮质骨厚度、骨密度（bone mineral density，BMD）、骨小梁数量（trabecular number，Tb.N）、骨小梁厚度（trabecular thickness，Tb.Th）、组织体积（tissue volume，TV）、组织表面积（tissue surface，TS）、骨体积（bone volume，BV）、骨表面积（bone surface，BS）、BV/TV、BS/BV、BS/TV、骨小梁分离度（trabecular separation，Tb.SP）等骨质参数，利用纳米压痕技术检测经煮沸后的肋骨与新鲜肋骨骨皮质的压痕硬度（indentation hardness，HIT）、压缩模量（elastic modulus，EIT）并分析上述参数在不同物种间的差异。
结果
2
骨质参数检测中，TV、TS、BS/TV、BS/BV、Tb.SP、Tb.N 6个参数能将人肋骨样本与猪、牛、羊、猴完全区分开来，而BV参数人肋骨样本只有与牛（
P
=0.001）、羊（
P
=0.001）差异有统计学意义；BS参数人类样本只与牛肋骨样本差异无统计学意义（
P
=0.304）；BV/TV参数人肋骨样本只与牛（
P
=0.025）、猴样本（
P
=0.002）差异有统计学意义；Tb.Th参数人只与牛（
P
=0.001）、猴样本（
P
=0.003）差异有统计学意义；BMD参数只有人肋骨样本与猪有统计学意义（
P
=0.006）；肋骨骨皮质厚度除人与牛肋骨骨皮质差异无统计学意义外（
P
=0.579），与其余物种差异均有统计学意
义。力学性能检测方面，煮沸组人肋骨HIT和EIT与猴肋骨（
P
=0.001）、与牛肋骨HIT差异具有统计学意义（
P
=0.001）外，与其余物种差异不具有统计学意义；新鲜组人肋骨除HIT、EIT与牛肋骨样本（
P
=0.999、
P
=0.997）以及与猴肋骨本的HIT（
P
=0.092）差异无统计学意义以外，与其余样本差异均有统计学意义样。
结论
2
利用Micro-CT骨质参数检测及纳米压痕力学性能检测均可鉴别人、猪、猴、牛、羊不同物种新鲜肋骨，对煮沸肋骨纳米压痕力学性能检测仅可区分人与其他动物肋骨。人与牛肋骨骨皮质厚度差异不明显，与猪、猴、羊的肋骨骨皮质厚度差异明显。
Abstract
Objective
2
To measure and compare the bone parameters and micromechanical parameters of ribs from different species，and to investigate the feasibility of these parameters in identifying different species.
Methods
2
The left fourth rib of humans，pigs，cattle，sheep，and monkeys was extracted，starting from about 1 cm outside the costal cartilage，with a length of 1 cm，and Micro-CT was used to measure the bone parameters of the ribs，including cortical bone thickness，bone mineral density（BMD），trabecular number（Tb.N），trabecular thickness（Tb.Th），tissue volume（TV），tissue surface（TS），bone volume（BV），bone surface （BS），BV/TV ratio，BS/BV ratio，BS/TV ratio，and trabecular separation（Tb.SP）. The nano-indentation technique was used to measure the indentation hardness（HIT） and elastic modulus（EIT） of the cortex of boiled ribs and fresh ribs，and these parameters were compared between different species.
Results
2
Among the bone parameters，the six parameters of TV，TS，BS/TV rate，BS/BV ratio，Tb.SP，and Tb.N could completely distinguish the human rib samples from the rib samples of pigs，cattle，sheep，and monkeys，whereas there was a significant difference in BV between the human rib samples and the rib samples of cattle（
P
=0.001） and sheep（
P
=0.001）. There was a significant difference in BS between the human rib samples and the cattle rib samples（
P
=0.304），and there was a significant difference in BV/TV ratio between the human rib samples and the rib
samples of cattle（
P
=0.025） and monkeys（
P
=0.002）. There was a significant difference in Tb.Th between the human rib samples and the rib samples of cattle（
P
=0.001） and monkeys（
P
=0.003），and there was a significant difference in BMD between the human rib samples and the pig rib samples（
P
=0.006）. There was a significant difference in cortical bone thickness between the human rib samples and the rib samples from all the other species except the cattle（
P
=0.579）. As for mechanical properties，there were significant differences in HIT and EIT between the human rib samples and the monkey rib samples in the boiled group（
P
=0.001），and there was a significant difference in HIT between the human rib samples and the cattle rib samples （
P
=0.001），but with no significant differences between the human rib samples and the rib samples from the other species；in the fresh group，there were no significant differences in HIT and EIT between the human rib samples and the cattle rib samples（
P
=0.999 and 0.997），and there was also no significant difference in HIT between the human rib samples and the monkey rib samples（
P
=0.092）； however，there were significant differences between the human rib samples and the rib samples from the other species.
Conclusion
2
The Micro-CT bone parameter testing and the nano-indentation mechanical performance testing can distinguish the fresh ribs of different species such as humans，pigs，monkeys，cattle，and sheep，and the nano-indentation mechanical performance testing of boiled ribs can only distinguish human ribs from the ribs of other animals. There is no significant difference in rib cortical thickness between humans and cattle，but there is a significant difference in rib cortical thickness between human ribs and the ribs from pigs，monkeys，and sheep.
关键词
Keywords
references
Delannoy Y ， Colard T ， Le Garff E ， et al . Effects of the environment on bone mass：a human taphonomic study [J ] . Leg Med ， 2016 ， 20 ： 61 - 67 .
Grela M ， Jakubczak A ， Kowalczyk M ， et al . Effectiveness of various methods of DNA isolation from bones and teeth of animals exposed to high temperature [J ] . J Forensic Leg Med ， 2021 ， 78 ： 102131 .
MacKinnon G . Forensic anthropology current methods and practice [J ] . J Forensic Leg Med ， 2014 ， 27 ： 87 - 88 .
Garvin HM ， Dunn R ， Sholts SB ， et al . Forensic tools for species identification of skeletal remains：metrics，statistics，and OsteoID [J ] . Biology ， 2021 ， 11 （ 1 ）： 25 .
Chen XY ， Lu LX ， Xiong XH ， et al . Development of a real-time PCR assay for the identification and quantification of bovine ingredient in processed meat products [J ] . Sci Rep ， 2020 ， 10 （ 1 ）： 2052 .
Keenan KE ， Mears CS ， Skedros JG . Utility of osteon circularity for determining species and interpreting load history in Primates and nonprimates [J ] . Am J Phys Anthropol ， 2017 ， 162 （ 4 ）： 657 - 681 .
Bailly M ， Féasson L ， Pereira B ， et al . Two new reliable immunohistochemical methods for simultaneous identification of capillaries，the three types of fibers and basal lamina in human skeletal muscle [J ] . Histochem Cell Biol ， 2020 ， 154 （ 3 ）： 327 - 337 .
Sohrabi H ， Cannizzo FT ， Pregel P ， et al . Tissue and species identification in minced meat and meat products from Italian commercial markets by DNA microarray and histological approach [J ] . Vet Ital ， 2020 ， 56 （ 2 ）： 77 - 85 .
Stan E ， Muresan CO ， Daescu E ， et al . A review of histological techniques for differentiating human bone from animal bone [J ] . Methods Protoc ， 2024 ， 7 （ 4 ）： 51 .
Taniguchi K ， Miyaguchi H . COL1A2 barcoding：bone species identification via shotgun proteomics [J ] . J Proteome Res ， 2024 ， 23 （ 1 ）： 377 - 385 .
Wang Q ， Li W ， Liu RN ， et al . Human and non-human bone identification using FTIR spectroscopy [J ] . Int J Legal Med ， 2019 ， 133 （ 1 ）： 269 - 276 .
Zhang JS ， Li XB ， Li LL ， et al . Y-STR analysis of highly degraded DNA from skeletal remains over 70 years old [J ] . Forensic Sci Res ， 2024 ， 9 （ 2 ）： owae020 .
Akhter MP ， Recker RR . High resolution imaging in bone tissue research-review [J ] . Bone ， 2021 ， 143 ： 115620 .
Kim Y ， Brodt MD ， Tang SY ， et al . MicroCT for scanning and analysis of mouse bones [J ] . Methods Mol Biol ， 2021 ， 2230 ： 169 - 198 .
郑仁建 ， 张一弓 ， 邵华英 ， 等 . Micro-CT重建时分析区域变化对颅顶骨骨量参数的影响 [J ] . 重庆医科大学学报 ， 2017 ， 42 （ 9 ）： 1077 - 1081 .
Zheng RJ ， Zhang YG ， Shao HY ， et al . Influence of analysis region on parameters of mouse calvarial during micro-CT reconstruction [J ] . J Chongqing Med Univ ， 2017 ， 42 （ 9 ）： 1077 - 1081 .
Feng T ， Ge YX ， Xie YJ ， et al . Detection of collagen by multi-wavelength photoacoustic analysis as a biomarker for bone health assessment [J ] . Photoacoustics ， 2021 ， 24 ： 100296 .
Remache D ， Semaan M ， Rossi JM ， et al . App lication of the Johnson -Cook plasticity model in the finite element simulations of the nanoindentation of the cortical bone [J ] . J Mech Behav Biomed Mater ， 2020 ， 101 ： 103426 .
Wang JW ， Yu K ， Li M ， et al . Application of nanoindentation technology in testing the mechanical properties of skull materials [J ] . Sci Rep ， 2022 ， 12 （ 1 ）： 8717 .
张玉凤 ， 王 璐 ， 辜向东 ， 等 . Mimics与Micro-CT内置软件测量小鼠颅骨缺损骨再生的对比研究 [J ] . 重庆医科大学学报 ， 2015 ， 40 （ 2 ）： 186 - 190 .
Zhang YF ， Wang L ， Gu XD ， et al . Evaluation on bone regeneration in mouse calvarial defect model using Mimics vs. Micro-CT scanner’s built-in software [J ] . J Chongqing Med Univ ， 2015 ， 40 （ 2 ）： 186 - 190 .
Oliver WC ， Pharr GM . An improved technique for determining hardness and elastic modulus using load and displacement sensing indentation experiments [J ] . J Mater Res ， 1992 ， 7 （ 6 ）： 1564 - 1583 .
Farlay D ， Bala Y ， Rizzo S ， et al . Bone remodeling and bone matrix quality before and after menopause in healthy women [J ] . Bone ， 2019 ， 128 ： 115030 .
张凌云 ， 王 玲 ， 刘有军 ， 等 . 基于显微磁共振成像和有限元分析的股骨近端微观力学行为研究 [J ] . 北京生物医学工程 ， 2020 ， 6 （ 2 ）： 111 - 116 .
Zhang LY ， Wang L ， Liu YJ ， et al . Study on micromechanical behavior of the proximal femur based on micro-magnetic resonance imaging and finite element analysis [J ] . Beijing Biomed Eng ， 2020 ， 6 （ 2 ）： 111 - 116 .
张馨元 ， 丁晓红 ， 段朋云 . 基于骨骼材料分布特征的髋关节有限元模型建立及其应力分析 [J ] . 北京生物医学工程 ， 2020 ， 39 （ 5 ）： 462 - 469 .
Zhang XY ， Ding XH ， Duan PY . Hip joint finite element modeling and its stress analysis based on the distribution characteristics of bone materials [J ] . Beijing Biomed Eng ， 2020 ， 39 （ 5 ）： 462 - 469 .
Zhang FZ ， Xie JL ， Wang GL ， et al . Anti-osteoporosis activity of Sanguinarine in preosteoblast MC3T3-E1 cells and an ovariectomized rat model [J ] . J Cell Physiol ， 2018 ， 233 （ 6 ）： 4626 - 4633 .
France DL . Comparative Bone Identification [M ] . Boca Raton ： CRC Press ， 2021 .
Pokines J . Identification of nonhuman remains received in a medical examiner setting . J Forensic Identif . 2015 ; 65 ( 3 ): 223 - 246 .
Hasap L ， Chotigeat W ， Pradutkanchana J ， et al . A novel，4-h DNA extraction method for STR typing of casework bone samples [J ] . Int J Legal Med ， 2020 ， 134 （ 2 ）： 461 - 471 .
Lavanya K ， Chandran SV ， Balagangadharan K ， et al . Temperature- and pH-responsive chitosan-based injectable hydrogels for bone tissue engineering [J ] . Mater Sci Eng C Mater Biol Appl ， 2020 ， 111 ： 110862 .
Eriksson AR ， Albrektsson T ， Albrektsson B . Heat caused by drilling cortical bone. Temperature measured in vivo in patients and animals [J ] . Acta Orthop Scand ， 1984 ， 55 （ 6 ）： 629 - 631 .
Yan J ， Clifton KB ， Jr Mecholsky JJ ， et al . Effect of temperature on the fracture toughness of compact bone [J ] . J Biomech ， 2007 ， 40 （ 7 ）： 1641 - 1645 .
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