Changes in immune status in decidua and peripheral blood during early pregnancy in women with advanced maternal age

Chen Ningxuan; Ma Xiaohui; Li Lian; Yang Zailin; Yi Ping; Xu Jing

doi:10.13406/j.cnki.cyxb.003622

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Changes in immune status in decidua and peripheral blood during early pregnancy in women with advanced maternal age

Clinical research | 更新时间：2025-10-23

- Changes in immune status in decidua and peripheral blood during early pregnancy in women with advanced maternal age
- Journal of Chongqing Medical University Vol. 49, Issue 11, Pages: 1443-1449(2024)
- 作者机构：
  
  1.重庆医科大学附属第三医院/捷尔医院，重庆 401120
  2.重庆大学附属肿瘤医院血液肿瘤中心，重庆 400030
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.003622
  CLC： R714
- Received：22 December 2023，
  
  Revised：2024-09-16，
  
  Accepted：18 September 2024，
  
  Published：28 November 2024
- 稿件说明：
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陈佞玄,马晓辉,李莲,等.高龄孕妇妊娠早期蜕膜及外周血免疫状态变化的研究[J].重庆医科大学学报,2024,49(11):1443-1449.

Chen Ningxuan,Ma Xiaohui,Li Lian,et al.Changes in immune status in decidua and peripheral blood during early pregnancy in women with advanced maternal age[J].Journal of Chongqing Medical University,2024,49(11):1443-1449.
陈佞玄,马晓辉,李莲,等.高龄孕妇妊娠早期蜕膜及外周血免疫状态变化的研究[J].重庆医科大学学报,2024,49(11):1443-1449. DOI： 10.13406/j.cnki.cyxb.003622.

Chen Ningxuan,Ma Xiaohui,Li Lian,et al.Changes in immune status in decidua and peripheral blood during early pregnancy in women with advanced maternal age[J].Journal of Chongqing Medical University,2024,49(11):1443-1449. DOI： 10.13406/j.cnki.cyxb.003622.

摘要

目的

观察高龄妊娠女性早孕期母体免疫环境的变化。

方法

收集2024年1月至4月于重庆医科大学附属第三医院行早期人工流产的女性外周血及蜕膜组织，分为高龄组和对照组，其中高龄组蜕膜标本16例、外周血12例，对照组蜕膜标本23例、外周血13例。通过流式细胞术检测外周血淋巴细胞亚群：CD3

T细胞、CD4

T细胞、CD8

T细胞、CD19

B细胞、自然杀伤（natural killer，NK）细胞以及蜕膜NK（decidual NK，dNK）细胞亚群：dNK1（CD103

CD18

CD39

）、dNK2（CD103

CD18

CD39

）、dNK3（CD103

CD18

CD39

）。并采用化学发光免疫分析技术，检测外周血血清中的细胞因子。

结果

相较于对照组，高龄组外周血中CD3

T细胞、CD19

B细胞、NK细胞数量和百分比无明显差异，但高龄组CD4

T细胞占比［（40.25±6.40）%］较对照组［（35.29±3.95）%］增多（

=0.030），高龄组CD8

T细胞占比［（21.83±6.20）%］较对照组［（26.69±4.76）%］减少（

=0.041）；2组的dNK占总淋巴细胞的百分比无明显差异，dNK1及dNK2细胞亚群在蜕膜NK细胞中占比无明显差异，但高龄组的dNK3亚群占比［（45.21±11.40）%］相较于对照组［（22.93±6.93）%］明显增多（

0.001）。高龄组血清细胞因子白细胞介素4（Interleukin-4，IL-4）水平低于对照组（

=0.017），同时干扰素γ（Interferon-γ，IFN-γ）水平也低于对照组（

=0.011），肿瘤坏死因子α（Tumor necrosis factor-α TNF-α）水平高于对照组（

=0.014）。

结论

高龄妊娠女性妊娠早期母体的免疫环境存在明显变化，dNK细胞亚群中dNK3细胞比例明显增高；外周血中存在CD4

T细胞比例增多和CD8

T细胞比例减少，IL-4和IFN-γ水平明显降低。

Abstract

Objective

To investigate the changes in maternal immune environment during early pregnancy in women with advanced maternal age.

Methods

Peripheral blood and decidual tissue samples were collected from the women who underwent early induced abortion in The Third Affiliated Hospital of Chongqing Medical University from January to April 2024，and the women were divided into advanced age group（16 decidual tissue samples and 12 peripheral blood samples） and control group（23 decidual tissue samples and 13 peripheral blood samples）. Flow cytometry was used to measure peripheral blood lymphocyte subsets（CD3

T cells，CD4

T cells，CD8

T cells，CD19

B cells，and natural killer［NK］ cells） and decidual NK（dNK） cell subsets including dNK1（CD103

CD18

CD39

），dNK2（C

D103

CD18

CD39

），and dNK3 （CD103

CD18

CD39

）. Chemiluminescent immunoassay was used to measure serum cytokines in peripheral blood.

Results

Compared with the control group，there was no significant difference in the number and percentage of CD3

T cells，CD19

B cells，and NK cells in the peripheral blood of the senior group，but the percentage of CD4

T cells in the senior group［（40.25±6.40）%］ was increased compared with that of the control group［（35.29±3.95）%］（

=0.030），and the percentage of CD8

T cells in the advance age group［（21.83±6.20）%］ was decreased（

=0.041） compared to the control group ［（26.69±4.76）%］. There was no significant difference between the two groups in the percentage of dNK in total lymphocytes and the percentages of dNK1 and dNK2 subsets in decidual NK cells ，but the advanced age group［（45.21±11.40）%］ had a significantly higher percentage of dNK3 subset than the control group［（22.93±6.93）%］（

0.001）. Compared with the control group in terms of serum cytokines，the advanced age group had significantly lower levels of interleukin-4（IL-4）（

=0.017） and interferon-γ（IFN-γ）（

=0.011）and a significantly higher level of tumor necrosis factor-α（

=0.014）.

Conclusion

There are significant changes in maternal immune environment during early pregnancy in women with advanced maternal age，with significant increases in the percentage of dNK3 cells in dNK cell subsets and the percentage of CD4

T cells in peripheral blood and significant reductions in the percentage of CD8

T cells and the levels of IL-4 and IFN-γ in the peripheral blood.

关键词

Keywords

references

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