Qiu Youjun,Deng Chun.Protective effects of progranulin in animal model of neonatal necrotizing enterocolitis[J].Journal of Chongqing Medical University,2024,49(9):1156-1162.
Qiu Youjun,Deng Chun.Protective effects of progranulin in animal model of neonatal necrotizing enterocolitis[J].Journal of Chongqing Medical University,2024,49(9):1156-1162. DOI: 10.13406/j.cnki.cyxb.003587.
Protective effects of progranulin in animal model of neonatal necrotizing enterocolitis
To investigate the protective role of progranulin(PGRN) in necrotizing enterocolitis(NEC) in neonatal mice.
Methods
2
Sixty 10-day-old wild-type C57 mice were divided into WT+Control group and WT+NEC group. Sixty 10-day-old PGRN-deficient(PGRN
-/-
) C57B/L mice were divided into PGRN
-/-
+Control group and PGRN
-/-
+NEC group. We measured PGRN mRNA and protein levels in the mouse intestine using quantitative PCR and enzyme-linked immunosor
bent assay(ELISA); recorded changes in the body weight of the mice; observed and scored the histological damage of the intestinal tissue with HE staining; measured the levels of inflammatory factors(C-C motif chemokine ligand 2[CCL2],tumor necrosis factor-alpha[TNF-α],and transforming growth factor-beta 1[TGF-β1]) in the mouse intestine; and assessed macrophage infiltration in the mouse intestine by immunofluorescence staining and flow cytometry.
Results
2
The mRNA and protein expression levels of PGRN in the WT+NEC group were significantly higher than those in the WT+Control group(
P
<
0.05). Compared with the WT+Control group,the pathology score of the intestinal tissue was significantly higher in the WT+NEC group; and the PGRN
-/-
+NEC group mice had a higher pathology score than the WT+NEC group(
P
<
0.05). Compared with the WT+Control group,the WT+NEC group showed significantly higher levels of pro-inflammatory CCL2 and TNF-α and a significantly lower level of anti-inflammatory TGF-β1;and compared with the WT+NEC group,the PGRN
-/-
+NEC group showed significantly higher CCL2 and TNF-α levels and a significantly lower TGF-β1 level(
P
<
0.05). Compared with the WT+Control group,the WT+NEC group had a significantly larger number of macrophages infiltrating in the lamina propria of the intestinal mucosa; and the PGRN
-/-
+NEC group had significantly more infiltrating macrophages than the WT+NEC group(
P
<
0.05).
Conclusion
2
PGRN is highly expressed in the intestinal tissue of NEC mice,and PGRN knockout exacerbates intestinal damage in NEC mice. PGRN deficiency may increase macrophage infiltration and inflammatory response.
关键词
Keywords
references
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