An experimental study of editable DNA nanoflowers inhibiting the proliferation，migration and invasion of Lewis lung carcinoma cells

Cao Yuchao; Liao Hongjian; Hu Can; Guo Jiajun; Du Yonghong

doi:10.13406/j.cnki.cyxb.003578

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An experimental study of editable DNA nanoflowers inhibiting the proliferation，migration and invasion of Lewis lung carcinoma cells

Basic research | 更新时间：2025-10-21

- An experimental study of editable DNA nanoflowers inhibiting the proliferation，migration and invasion of Lewis lung carcinoma cells
- Journal of Chongqing Medical University Vol. 49, Issue 9, Pages: 1105-1112(2024)
- 作者机构：
  
  重庆医科大学生物医学工程学院/超声医学工程国家重点实验室，重庆 400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.003578
  CLC： R734.2
- Received：10 November 2023，
  
  Revised：2024-12-22，
  
  Accepted：26 February 2024，
  
  Published：28 September 2024
- 稿件说明：
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曹宇超,廖洪建,胡璨,等.可编辑DNA纳米花抑制LLC细胞增殖、迁移及侵袭的实验研究[J].重庆医科大学学报,2024,49(9):1105-1112.

Cao Yuchao,Liao Hongjian,Hu Can,et al.An experimental study of editable DNA nanoflowers inhibiting the proliferation，migration and invasion of Lewis lung carcinoma cells[J].Journal of Chongqing Medical University,2024,49(9):1105-1112.
曹宇超,廖洪建,胡璨,等.可编辑DNA纳米花抑制LLC细胞增殖、迁移及侵袭的实验研究[J].重庆医科大学学报,2024,49(9):1105-1112. DOI： 10.13406/j.cnki.cyxb.003578.

Cao Yuchao,Liao Hongjian,Hu Can,et al.An experimental study of editable DNA nanoflowers inhibiting the proliferation，migration and invasion of Lewis lung carcinoma cells[J].Journal of Chongqing Medical University,2024,49(9):1105-1112. DOI： 10.13406/j.cnki.cyxb.003578.

摘要

目的

探究载有阿霉素（doxorubicin，DOX）和缺氧诱导因子-1α（hypoxia inducible factor-1α，HIF-1α）反义寡核苷酸的DNA纳米花（DNA nanoflower，DF）对小鼠肺癌（lewis lung carcinoma，LLC）细胞增殖、迁移及侵袭能力的影响。

方法

通过滚环扩增反应（rolling circle amplification，RCA）合成含有HIF-1α反义寡核苷酸的DHA-DF，并检测其物理特性；负载DOX后获得载DOX纳米花（DOX-loaded DNA nanoflower，DDF）。通过细胞计数试剂（cell counting kit-8，CCK-8）评价DHA-DDF对LLC细胞活性的影响；通过划痕实验和Transwell小室实验检测DHA-DDF对LLC细胞迁移及侵袭能力的影响；通过蛋白质印记实验和定量聚合酶链反应检测LLC细胞HIF-1α的表达水平。

结果

DHA-DF为多孔花瓣状球形颗粒，粒径为（421.26±7.90） nm；与游离DOX和DA-DDF相比，经DHA-DDF处理后的LLC细胞HIF-1α蛋白及mRNA的表达量明显下降（均

0.05），且其细胞活性、划痕愈合面积及侵袭细胞数目明显减少（均

0.05）。

结论

DHA-DDF能有效降低HIF-1α的表达并抑制LLC细胞的增殖、迁移和侵袭，有望为临床肿瘤治疗提供新的思路。

Abstract

Objective

To investigate the effects on the proliferation，migration and invasion of Lewis lung carcinoma（LLC） cells induced by DNA nanoflowers（DFs） carrying doxorubicin（DOX） and hypoxia inducible factor-1α（HIF-1α） antisense oligonucleotides.

Methods

DFs containing HIF-1α antisense oligonucleotides（DHA-DF） were synthesized by rolling circle amplification，and the physical properties of DHA-DF were examined. DOX was loaded into DFs to form DDF.We evaluated the cell viability of LLC cells treated with DHA-DDF by using Cell Counting Kit-8； assessed the effects of DHA-DDF on the migration and invasion of LLC cells by using scratch assay and Transwell assay； and measured the expression level of HIF-1α in LLC cells by Western blot and quantitative polymerase chain reaction.

Results

DHA-DF appeared flower-like porous spherical particles with a size of （421.26±7.90） nm. Compare with free DOX and DA-DDF，DHA-DDF significantly decreased the mRNA and protein expression of HIF-1α in LLC cells，and significantly reduced cell viability，the area of wound healed and the number of invasive cells（all

0.05）.

Conclusion

DHA-DDF could effectively reduce the expression of HIF-1α，and inhibit the proliferation，migration and invasion of LLC cells，providing new ideas for clinical treatment of tumors.

关键词

Keywords

references

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