Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis

Liu Jie; Xie Xingming; Niu Hongxia; Yang Xianzhi

doi:10.13406/j.cnki.cyxb.003458

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Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis

Basic research | 更新时间：2025-10-22

- Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis
- Journal of Chongqing Medical University Vol. 49, Issue 3, Pages: 276-282(2024)
- 作者机构：
  
  遵义医科大学第三附属医院/遵义市第一人民医院胃肠外科，遵义 563000
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.003458
  CLC： R735.2
- Received：27 June 2023，
  
  Revised：2023-11-10，
  
  Accepted：28 November 2023，
  
  Published：28 March 2024
- 稿件说明：
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刘洁,谢兴明,钮洪霞,等.LncRNA MYLK-AS1调节miR-141-3p/STMN1轴对胃癌细胞增殖、凋亡和侵袭的影响[J].重庆医科大学学报,2024,49(3):276-282.

Liu Jie,Xie Xingming,Niu Hongxia,et al.Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis[J].Journal of Chongqing Medical University,2024,49(3):276-282.
刘洁,谢兴明,钮洪霞,等.LncRNA MYLK-AS1调节miR-141-3p/STMN1轴对胃癌细胞增殖、凋亡和侵袭的影响[J].重庆医科大学学报,2024,49(3):276-282. DOI： 10.13406/j.cnki.cyxb.003458.

Liu Jie,Xie Xingming,Niu Hongxia,et al.Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis[J].Journal of Chongqing Medical University,2024,49(3):276-282. DOI： 10.13406/j.cnki.cyxb.003458.

摘要

目的

探讨长链非编码RNA（long non-coding RNA，LncRNA）肌球蛋白轻链激酶反义RNA1（myosin light chain kinase antisense RNA1，MYLK-AS1）调节miR-141-3p/微管不稳定蛋白1（stathmin 1，STMN1）轴对胃癌细胞增殖、凋亡和侵袭的影响。

方法

将HGC27细胞分为NC组、si-NC组、si-MYLK-AS1组、si-MYLK-AS1+inhibitor NC组、si-MYLK-AS1+miR-141-3p inhibitor组。双萤光素酶报告基因实验检测LncRNA MYLK-AS1、miR-141-3p、STMN1的关系；qRT-PCR检测HGC27细胞中LncRNA MYLK-AS1、miR-141-3p表达；CCK-8法检测HGC27细胞增殖情况；流式细胞术检测HGC27细胞凋亡；使用Transwell实验评估了HGC27细胞的侵袭和迁移能力，并统计了穿透基质膜的细胞数量；同时采用Western blot技术检测了HGC27细胞中STMN1、E-cadherin、Vimentin及N-cadherin这几种蛋白表达量的变化。

结果

HGC27细胞中LncRNA MYLK-AS1、STMN1水平高于GES-1细胞（

0.05），miR-141-3p水平低于GES-1细胞（

0.05）。si-MYLK-AS1组HGC27细胞

450 nm

值、迁移、侵袭细胞数量、LncRNA MYLK-AS1表达量、STMN1、N-cadherin、Vimentin蛋白水平低于NC组、si-NC组（

0.05），HGC27细胞凋亡率、miR-141-3p表达量、E-cadherin蛋白水平高于NC组、si-NC组（

0.05）；而miR-141-3p低表达减弱了沉默LncRNA MYLK-AS1抑制HGC27细胞发展的作用；LncRNA MYLK-AS1靶向调节miR-141-3p/STMN1轴。

结论

LncRNA MYLK-AS1可能通过上调microRNA-141-3p的表达水平，间接导致STMN1基因表达受到抑制，这一过程可能对胃癌细胞的增殖、凋亡及侵袭特性产生明显影响。

Abstract

Objective

To investigate the impacts of long non-coding RNA（LncRNA） myosin light chain kinase antisense RNA1 （MYLK-AS1） on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/stathmin 1（STMN1） axis.

Methods

HGC27 cells were grouped into negative control（NC） group，si-NC group，si-MYLK-AS1 group，si-MYLK-AS1+inhibitor NC group，and si-MYLK-AS1+miR-141-3p inhibitor group. The relationship among LncRNA MYLK-AS1，miR-141-3p，and STMN1 was determined by the dual-luciferase reporter gene assay；the expression of LncRNA MYLK-AS1 and miR-141-3p in HGC27 cells was measured by qRT-PCR；the CCK-8 method and flow cytometry were usde to determined the proliferation and apoptosis of HGC27 cells，respectively；the numbers of invading and migrating HGC27 cells were counted by Transwell；the protein levels of STMN1，E-cadherin，Vimentin，and N-cadherin in HGC27 cells were measured by Western blot.

Results

Compared with GES-1 cells，the levels of LncRNA MYLK-AS1 and STMN1 in HGC27 cells were significantly up-regulated（

0.05），and the level of miR-141-3p was significantly down-regulated（

0.05）. Compared with the NC group and si-NC group，the si-MYLK-AS1 group showed significant decreases in the optical density at 450 nm，numbers of migrating and invading cells，expression level of LncRNA MYLK-AS1，and protein levels of STMN1，N-cadherin，and Vimentin（

0.05），as well as significant increases in the apoptosis rate，expression level of miR-141-3p，and protein level of E-cadherin（

0.05）. However，the low expression of miR-141-3p attenuated the inhibition of HGC27 cell development by silencing LncRNA MYLK-AS1；LncRNA MYLK-AS1 regulated the miR-141-3p/STMN1 axis in a targeted manner.

Conclusion

Silencing LncRNA MYLK-AS1 may inhibit the expression of STMN1 by up-regulating miR-141-3p，thus affecting the proliferation，apoptosis，and invasion of gastric cancer cells.

关键词

Keywords

references

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