Jiang Wen,Deng Qionghua,Mei Song.Effects of M1 microglia-derived exosomes on the function of the blood-brain barrier[J].Journal of Chongqing Medical University,2024,49(2):153-157.
Jiang Wen,Deng Qionghua,Mei Song.Effects of M1 microglia-derived exosomes on the function of the blood-brain barrier[J].Journal of Chongqing Medical University,2024,49(2):153-157. DOI: 10.13406/j.cnki.cyxb.003419.
Effects of M1 microglia-derived exosomes on the function of the blood-brain barrier
To investigate the effects of M1 microglia-derived exosomes(M1-exo) on the function of an in-vitro blood-brain barrier(BBB) model and the expression of tight junction proteins between vascular endothelial cells.
Methods
2
Mouse microglia-derived BV2 cells were stimulated with lipopolysaccharide to be polarized towards the M1 phenotype,and exosomes were isolated and extracted after the phenotype was confirmed by flow cytometry. An in-vitro BBB model was constructed using the mouse brain microvascular endothelial cell line b.End3 and primary cultured mouse astrocytes. The BBB model was co-cultured with exosomes of different sources in three groups:normal culture for b.End3 cells(b.End3 group);b.End3 cells + 25 μg/mL exosomes derived from normal BV-2 cells(b.End3+BV2-exo group);and b.End3 cells+25 μg/mL M1-exo(b.End3+M1-exo group). Trans-endothelial electrical resistance(TEER) and Lucifer yellow permeability were examined. The expression of tight junction complex proteins(claudin-1,occludin,zonula occludens-1[ZO-1],and junctional adhesion molecules) was measured by Western blot.
Results
2
Microglia were successfully polarized to the M1 type,and the positivity rate of the M1 marker CD16/32 was significantly increased compared with the control group(
P
=0.023). After co-culture with M1-exo,the TEER of the in-vitro BBB model was significantly decreased(
P
<
0.001),and Lucifer yellow permeability was significantly increased(
P
<
0.001). Compared with the b.End3 group and b.End3+BV2-exo group,the b.End3+M1-exo group showed significantly decreased expression levels of claudin-1,occludin,and ZO-1.
Conclusion
2
M1-exo can disrupt the integrity of BBB and interfere with its normal function.
关键词
Keywords
references
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