Knock-down RBX1 inhibits the proliferation，migration，and invasion of pancreatic cancer cells and promotes cell apoptosis by down-regulating PHF5A

Zhang Jing; Yan Youyou; Xu Dongchao; Zhang Bo

doi:10.13406/j.cnki.cyxb.003347

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Knock-down RBX1 inhibits the proliferation，migration，and invasion of pancreatic cancer cells and promotes cell apoptosis by down-regulating PHF5A

Basic research | 更新时间：2025-10-22

- Knock-down RBX1 inhibits the proliferation，migration，and invasion of pancreatic cancer cells and promotes cell apoptosis by down-regulating PHF5A
- Journal of Chongqing Medical University Vol. 48, Issue 11, Pages: 1314-1322(2023)
- 作者机构：
  
  1.浙江大学医学院附属杭州市第一人民医院，转化医学研究中心，杭州 310000
  2.浙江大学医学院附属杭州市第一人民医院，消化内科，杭州 310000
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.003347
  CLC： R730.23;R735.9
- Received：27 April 2023，
  
  Revised：2023-08-03，
  
  Accepted：12 September 2023，
  
  Published：28 November 2023
- 稿件说明：
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张菁,阎优优,徐栋超,等.敲低RBX1通过下调PHF5A抑制胰腺癌细胞增殖，迁移和侵袭并促进凋亡[J].重庆医科大学学报,2023,48(11):1314-1322.

Zhang Jing,Yan Youyou,Xu Dongchao,et al.Knock-down RBX1 inhibits the proliferation，migration，and invasion of pancreatic cancer cells and promotes cell apoptosis by down-regulating PHF5A[J].Journal of Chongqing Medical University,2023,48(11):1314-1322.
张菁,阎优优,徐栋超,等.敲低RBX1通过下调PHF5A抑制胰腺癌细胞增殖，迁移和侵袭并促进凋亡[J].重庆医科大学学报,2023,48(11):1314-1322. DOI： 10.13406/j.cnki.cyxb.003347.

Zhang Jing,Yan Youyou,Xu Dongchao,et al.Knock-down RBX1 inhibits the proliferation，migration，and invasion of pancreatic cancer cells and promotes cell apoptosis by down-regulating PHF5A[J].Journal of Chongqing Medical University,2023,48(11):1314-1322. DOI： 10.13406/j.cnki.cyxb.003347.

摘要

目的

研究环盒蛋白1（ring-finger protein 1，RBX1）对胰腺癌（pancreatic adenocarcinoma，PAAD）细胞增殖、凋亡、迁移和侵袭的作用及其潜在的作用机制。

方法

利用基因表达谱交互式分析网站（gene expression profile interactive analysis website，GEPIA），实时荧光定量聚合酶链反应（quantitative reverse transcription-polymerase chain reaction，qRT-PCR）、Western blot检测RBX1在PAAD组织、细胞中的表达和预后价值。使用细胞计数试剂盒-8（cell counting kit-8，CCK-8）、细胞克隆形成能力实验、脱氧核糖核苷酸末端转移酶介导的缺口末端标记法（terminal deoxynucleotidyl transferase dUTP nick end labeling，TUNEL）、细胞划痕实验、Transwell实验检测RBX1敲低对胰腺癌-1（pancreatic carcinoma-1，PANC-1）细胞增殖、凋亡、迁移和侵袭的影响。通过LinkedOmics网站研究PAAD中RBX1和PHD锌指结构域蛋白5A（plant homodomain finger-like domain-containing protein 5A，PHF5A）的相关性。qRT-PCR和Western blot检测RBX1敲低后PHF5A的表达，进行功能拯救实验。

结果

RBX1在PAAD组织和细胞系中高表达，与不良预后相关（

0.05）。RBX1敲低抑制PANC-1细胞增殖、迁移和侵袭，促进细胞凋亡（

0.05）。RBX1和PHF5A在PAAD中具有相关性（

=0.692），且RBX1正向调控PHF5A表达（

0.05）。功能拯救实验表明PHF5A过表达部分逆转了RBX1敲低对PANC-1细胞增殖、迁移、侵袭和凋亡的作用（

0.05）。

结论

RBX1敲低通过下调PHF5A表达抑制PANC-1细胞增殖、迁移和侵袭，促进凋亡。

Abstract

Objective

To explore the effect of ring box protein-1（RBX1） on the proliferation，apoptosis，migration，and invasion of pancreatic adenocarcinoma（PAAD） cells and the potential mechanism of action.

Methods

The gene expression profile interactive analysis website，quantitative real-time reverse-transcription polymerase chain reaction（qRT-PCR），and Western blot were used to measure the expression of RBX1 in PAAD tissues and cells and to determine its prognostic value. Cell Counting Kit-8，colony formation assay，terminal deoxynucleotidyl transferase dUTP nick end labeling，wound healing assay，and transwell assay were applied to evaluate the effects of RBX1 knockdown on the proliferation，apoptosis，migration，and invasion of pancreatic carcinoma-1（PANC-1） cells. LinkedOmics website was used to investigate the correlation between RBX1 and plant homodomain finger-like domain-containing protein 5A（PHF5A） in PAAD. Functional rescue experiments were performed by measuring the expression of PHF5A after RBX1 knockdown by qRT-PCR and Western blot.

Results

RBX1 expression was up-regulated in PAAD tissues and cells，which was associated with a poor prognosis（

0.05）. RBX1 knockdown inhibited the proliferation，migration，and invasion of PANC-1 cells and promoted cell apoptosis（

0.05）. RBX1 was associated with PHF5A in PAAD（

=0.6918），and RBX1 positively regulated th

e expression of PHF5A（

0.05）. Functional rescue experiments showed that PHF5A overexpression partially reversed the effects of RBX1 knockdown on the proliferation，migration，invasion，and apoptosis of PANC-1 cells（

0.05）.

Conclusion

RBX1 knockdown inhibits the proliferation，migration，and invasion of PANC-1 cells and promotes cell apoptosis by down-regulating PHF5A.

关键词

Keywords

references

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