miR-149-5p targets AEBP1 to inhibit migration and invasion of gastric cancer cells

Chen Yan; Jiang Lei; Min Guangtao; Wang Jun; Chen Wei; Wang Hongpeng; Wang Xiangwen

doi:10.13406/j.cnki.cyxb.003346

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miR-149-5p targets AEBP1 to inhibit migration and invasion of gastric cancer cells

Basic research | 更新时间：2025-10-22

- miR-149-5p targets AEBP1 to inhibit migration and invasion of gastric cancer cells
- Journal of Chongqing Medical University Vol. 48, Issue 10, Pages: 1159-1165(2023)
- 作者机构：
  
  1.兰州大学第一临床医学院，兰州 730030
  2.兰州大学第一医院普外科六病区，兰州 730030
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.003346
  CLC： R735.2
- Received：18 August 2023，
  
  Revised：2023-08-20，
  
  Accepted：30 September 2023，
  
  Published：28 October 2023
- 稿件说明：
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陈燕,姜雷,闵光涛,等.miR-149-5p通过靶向AEBP1调控胃癌细胞的迁移侵袭[J].重庆医科大学学报,2023,48(10):1159-1165.

Chen Yan,Jiang Lei,Min Guangtao,et al.miR-149-5p targets AEBP1 to inhibit migration and invasion of gastric cancer cells[J].Journal of Chongqing Medical University,2023,48(10):1159-1165.
陈燕,姜雷,闵光涛,等.miR-149-5p通过靶向AEBP1调控胃癌细胞的迁移侵袭[J].重庆医科大学学报,2023,48(10):1159-1165. DOI： 10.13406/j.cnki.cyxb.003346.

Chen Yan,Jiang Lei,Min Guangtao,et al.miR-149-5p targets AEBP1 to inhibit migration and invasion of gastric cancer cells[J].Journal of Chongqing Medical University,2023,48(10):1159-1165. DOI： 10.13406/j.cnki.cyxb.003346.

摘要

目的

探讨miR-149-5p对胃癌细胞迁移侵袭能力的影响及分子机制。

方法

qRT-PCR检测胃癌细胞株和组织标本中miR-149-5p的表达，分析其表达水平与胃癌患者临床病理特征参数及预后的相关性，建立过表达和干扰miR-149-5p的胃癌细胞株，Transwell实验检测miR-149-5p表达水平对胃癌癌细胞迁移侵袭能力的影响；生物信息学网站预测miR-149-5p的靶基因，采用荧光素酶报告基因实验加以验证。

结果

miR-149-5p在胃癌组织和细胞株中均低表达（均

0.05）。miR-149-5p的表达水平与胃癌患者的浸润深度（

=0.016）、淋巴结转移（

=0.001）和TNM分期（

=0.023）相关。miR-149-5p低表达是胃癌患者总生存期的独立危险因素。与对照组相比，miR-149-5p mimics明显抑制胃癌细胞的迁移侵袭能力（均

0.01），而转染miR-149-5p inhibitors后得到了相反的结果（均

0.05）。miR-149-5p靶向调控脂肪细胞增强结合蛋白1（Adipocyte enhancer binding protein 1，AEBP1）蛋白的表达。荧光素酶报告基因实验表明，miR-149-5p明显抑制了野生型AEBP1载体的荧光素酶活性（

0.01），而突变型的荧光素酶活性不受影响。敲低AEBP1可部分逆转下调miR-149-5p对胃癌细胞迁移侵袭能力的影响。

结论

miR-149-5p在胃癌组织中低表达，通过靶向AEBP1负性调控胃癌癌细胞的迁移侵袭。

Abstract

Objective

To investigate the effects of miR-149-5p in regulating the migration and invasion ability of gastric cancer（GC） cells and the molecular mechanism.

Methods

The expression of miR-149-5p in GC cell lines and tissue specimens was measured by qRT-PCR to analyze the relationship between miR-149-5p expression and the clinicopathological parameters and prognosis of patients with GC. We separately overexpressed and knocked down miR-149-5p in GC cells to investigate the expression level of miR-149-5p on the migration and invasion ability of GC cells using Transwell assay. The target genes of miR-149-5p were predicted using bioinformatic tools，which were verified using luciferase reporter gene assay.

Results

The expression of miR-149-5p was significantly down-regulated in both GC tissues and cell lines（

0.05）. The expression level of miR-149-5p was significantly correlated with

the depth of invasion（

=0.016），lymph node metastasis（

=0.001），and TNM stage（

=0.023） of patients with GC. Low expression of miR-149-5p was an independent risk factor for the overall survival of patients with GC. Compared with the control group，miR-149-5p mimics significantly inhibited the migration and invasion ability of gastric cancer cells（

0.01），while transfection with miR-149-5p inhibitors produced the opposite effects（

0.05）. miR-149-5p targeted the expression of adipocyte enhancer-binding protein 1（AEBP1）. The luciferase reporter gene assay showed that miR-149-5p significantly inhibited the luciferase activity of the wild-type AEBP1 vector（

0.01），with no effects on the luciferase activity of the mutant type. Knockdown of AEBP1 could partly reverse the effects of downregulating miR-149-5p on the migration and invasion ability of GC cells.

Conclusion

miR-149-5p is lowly expressed in GC tissues，which can negatively regulate the migration and invasion of GC cells by targeting AEBP1 protein expression.

关键词

Keywords

references

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