The diagnostic role of flow cytometry immunophenotyping analysis in 11 cases of aggressive NK-cell leukemia

Yang Ying; Zhang Xin; Liao Hongyan

doi:10.13406/j.cnki.cyxb.003273

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The diagnostic role of flow cytometry immunophenotyping analysis in 11 cases of aggressive NK-cell leukemia

Clinical research | 更新时间：2025-10-21

- The diagnostic role of flow cytometry immunophenotyping analysis in 11 cases of aggressive NK-cell leukemia
- Journal of Chongqing Medical University Vol. 48, Issue 7, Pages: 793-797(2023)
- 作者机构：
  
  四川大学华西医院实验医学科，成都 610041
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.003273
  CLC： R446
- Received：08 June 2022，
  
  Revised：2023-03-31，
  
  Accepted：13 April 2023，
  
  Published：28 July 2023
- 稿件说明：
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杨莹,张欣,廖红艳.流式免疫表型分析在11例侵袭性NK细胞白血病中的诊断价值分析[J].重庆医科大学学报,2023,48(7):793-797.

Yang Ying,Zhang Xin,Liao Hongyan.The diagnostic role of flow cytometry immunophenotyping analysis in 11 cases of aggressive NK-cell leukemia[J].Journal of Chongqing Medical University,2023,48(7):793-797.
杨莹,张欣,廖红艳.流式免疫表型分析在11例侵袭性NK细胞白血病中的诊断价值分析[J].重庆医科大学学报,2023,48(7):793-797. DOI： 10.13406/j.cnki.cyxb.003273.

Yang Ying,Zhang Xin,Liao Hongyan.The diagnostic role of flow cytometry immunophenotyping analysis in 11 cases of aggressive NK-cell leukemia[J].Journal of Chongqing Medical University,2023,48(7):793-797. DOI： 10.13406/j.cnki.cyxb.003273.

摘要

目的

探讨流式细胞术（flow cytometry，FCM）免疫分型及临床和实验室特征对侵袭性NK细胞白血病（aggressive NK-cell leukemia，ANKL）的诊断价值。

方法

回顾性分析2019年1月至2019年12月在四川大学华西医院确诊的11例ANKL患者临床资料，包括骨髓细胞形态学、流式免疫表型及病理结果。

结果

所有患者均出现发热症状并均伴噬血细胞淋巴组织细胞增生症（hemophagocytic lymphohistocytosis，HLH）。8例骨髓涂片中可见噬血细胞。流式细胞学示11例ANKL患者免疫表型均为：CD2

sCD3

CD4

CD8

CD56

CD19

，CD45和CD56表达强度增高（

=0.048）、细胞体积偏大（

=0.000），5例CDl6

，6例CD7表达缺失或部分缺失。8例行免疫组化检测，结果均为：CD3ε

、粒酶B

及EBER1/2-ISH

。

结论

ANKL的免疫表型有独特的特征，在ANKL的诊断和鉴别诊断中具有重要意义。综合分析病例的临床特点、细胞形态学、免疫表型等检查结果可实现对ANKL的及时诊断和鉴别诊断。

Abstract

Objective

To explore the diagnostic role of flow cytometry（FCM） immunophenotyping and the clinical and laboratory characteristics of aggressive NK-cell leukemia（ANKL）.

Methods

A retrospective analysis was performed on clinical data of 11 patients diagnosed of ANKL in West China Hospital from January 2019 to December 2019. Bone marrow cytomorphology，immunophenotype and pathological findings were collected and analyzed.

Results

All patients suffered from fever accompanied with hemophagocytic lymphohistiocytosis（HLH）. Hemopha

gocytic cells were found in bone marrow smears of 8 cases. FCM revealed an immunophenotype of CD2

sCD3

CD4

CD8

CD56

CD19

in all 11 cases of ANKL. The expression of CD45 and CD56 increased（

=0.048），and the volume of cells was relatively large（

=0.000）. CD16

was found in 5 patients，and partial or complete loss of CD7 expression was identified in 6 patients. Immunohistochemical tests were performed on 8 patients，and the results were CD3ε

，granzyme B

，and EBER1/2-ISH

Conclusion

The immunophenotype of ANKL has unique characteristics and is of great significance in the diagnosis and differential diagnosis of ANKL. Timely and differential diagnosis of ANKL can be achieved by comprehensive analysis of clinical characteristics， bone marrow cell morphology，immunophenotype and immunohistochemistry.

关键词

Keywords

references

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