Histopathology of temporomandibular joint osteoarthritis in rats under abnormal mechanical load

He Dong; Li Yanhua; Yao Hongying

doi:10.13406/j.cnki.cyxb.003259

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Histopathology of temporomandibular joint osteoarthritis in rats under abnormal mechanical load

Basic research | 更新时间：2025-10-21

- Histopathology of temporomandibular joint osteoarthritis in rats under abnormal mechanical load
- Journal of Chongqing Medical University Vol. 48, Issue 6, Pages: 641-646(2023)
- 作者机构：
  
  1.苏州大学附属儿童医院口腔科，苏州 215000
  2.苏州口腔医院牙周科，苏州 215000
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.003259
  CLC： R782.6
- Received：13 September 2022，
  
  Revised：2023-04-08，
  
  Accepted：04 May 2023，
  
  Published：28 June 2023
- 稿件说明：
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何东,李艳华,姚红英.异常机械负荷加重大鼠颞下颌关节骨关节炎的组织病理学研究[J].重庆医科大学学报,2023,48(6):641-646.

He Dong,Li Yanhua,Yao Hongying.Histopathology of temporomandibular joint osteoarthritis in rats under abnormal mechanical load[J].Journal of Chongqing Medical University,2023,48(6):641-646.
何东,李艳华,姚红英.异常机械负荷加重大鼠颞下颌关节骨关节炎的组织病理学研究[J].重庆医科大学学报,2023,48(6):641-646. DOI： 10.13406/j.cnki.cyxb.003259.

He Dong,Li Yanhua,Yao Hongying.Histopathology of temporomandibular joint osteoarthritis in rats under abnormal mechanical load[J].Journal of Chongqing Medical University,2023,48(6):641-646. DOI： 10.13406/j.cnki.cyxb.003259.

摘要

目的

研究异常机械负荷在大鼠颞下颌关节软骨退变中的作用。

方法

36只8周龄雌性SD大鼠随机分为4组，分别为对照组（control group，Con组）、Ⅱ型胶原蛋白酶组（collagenase type Ⅱ group，CⅡ组）、大张嘴组（wide mouth opening group，MS组）和Ⅱ型胶原酶+大张嘴组（collagenase type Ⅱ+wide mouth opening group，CⅡ+MS组）。HE染色、番红O-固绿染色观测软骨组织病理学变化，免疫组化实验检测炎性因子基质金属蛋白酶3（matrix metalloproteinase 3，MMP3）和聚蛋白多糖酶5（a disintegrin and metalloproteinase with thrombospondin motifs 5，ADAMTS5）的表达。

结果

与Con组相比，CⅡ、MS和CⅡ+MS组大鼠颞下颌关节软骨后区总厚度、钙化层厚度和番红O阳性面积比均显著降低（均

0.05），CⅡ、MS和CⅡ+MS组中MMP3和ADAMTS5的阳性表达均显著增高（均

=0.000）；与CⅡ和MS组相比，CⅡ+MS组软骨钙化层厚度显著降低（均

=0.000），ADAMTS5表达显著增高（均

=0.000）；MS和CⅡ+MS组软骨后区潮线呈波浪状。

结论

异常机械负荷促使髁突软骨后区退变及改建，这将有助于异常负荷致颞下颌关节骨关节炎的机制研究。

Abstract

Objective

To investigate the impact of abnormal mechanical load on cartilage degeneration of the temporomandibular joint （TMJ） in rats.

Methods

A total of 36 female Sprague-Dawley rats，aged 8 weeks，were randomly divided into control group（Con group），collagenase type Ⅱ group（CⅡ group），wide mouth opening group（MS group），and collagenase type Ⅱ+wide mouth opening group （CII+MS group）. HE staining and safranin O-fast green staining were used to observe the histopathological changes of cartilage， and immunohistochemistry was used to measure the expression levels of the inflammatory factors matrix metalloproteinase 3（MMP3） and A disintegrin and metalloproteinase with thrombospondin motifs 5（ADAMTS5）.

Results

Compared with the Con group，the CII，MS， and CII+MS groups had significant reductions in the total thickness，calcified layer thickness， and safranin O-positive area ratio of the TMJ posterior zone（

0.05） and significant increases in the positive expression of MMP3 and ADAMTS5（

=0.000）. Compared with the CⅡ and MS groups，the CⅡ+MS group had a significant reduction in the calcified layer thickness of cartilage（

=0.000） and a significant increase in the expression of ADAMTS5（

=0.000）. The MS and CII+MS groups had a wavy tidal line in the posterior zone of cartilage.

Conclusion

Abnormal mechanical load promotes the degeneration and reconstruction of the posterior zone of condylar cartilage，which may help with the research on the mechanism of TMJ osteoarthritis caused by abnormal mechanical load.

关键词

Keywords

references

Delpachitra SN ， Dimitroulis G . Osteoarthritis of the temporomandibular joint：a review of aetiology and pathogenesis [J ] . Br J Oral Maxillofac Surg ， 2022 ， 60 （ 4 ）： 387 - 396 .

Chen J ， Sorensen KP ， Gupta T ， et al . Altered functional loading causes differential effects in the subchondral bone and condylar cartilage in the temporomandibular joint from young mice [J ] . Osteoarthritis Cartilage ， 2009 ， 17 （ 3 ）： 354 - 361 .

Fujisawa T ， Kuboki T ， Kasai T ， et al . A repetitive，steady mouth opening induced an osteoarthritis-like lesion in the rabbit temporomandibular joint [J ] . J Dent Res ， 2003 ， 82 （ 9 ）： 731 - 735 .

Maldonado M ， Nam J . The role of changes in extracellular matrix of cartilage in the presence of inflammation on the pathology of osteoarthritis [J ] . Biomed Res Int ， 2013 ， 2013 ： 284873 .

Durigova M ， Nagase H ， Mort JS ， et al . MMPs are less efficient than ADAMTS5 in cleaving aggrecan core protein [J ] . Matrix Biol ， 2011 ， 30 （ 2 ）： 145 - 153 .

Sobue T ， Yeh WC ， Chhibber A ， et al . Murine TMJ loading causes increased proliferation and chondrocyte maturation [J ] . J Dent Res ， 2011 ， 90 （ 4 ）： 512 - 516 .

Miquelestorena-Standley E ， Jourdan ML ， Collin C ， et al . Effect of decalcification protocols on immunohistochemistry and molecular analyses of bone samples [J ] . Mod Pathol ， 2020 ， 33 （ 8 ）： 1505 - 1517 .

Li BC ， Guan GZ ， Mei L ， et al . Pathological mechanism of chondrocytes and the surrounding environment during osteoarthritis of temporomandibular joint [J ] . J Cell Mol Med ， 2021 ， 25 （ 11 ）： 4902 - 4911 .

Meng JH ， Ma XC ， Ma DL ， et al . Microarray analysis of differential gene expression in temporomandibular joint condylar cartilage after experimentally induced osteoarthritis [J ] . Osteoarthritis Cartilage ， 2005 ， 13 （ 12 ）： 1115 - 1125 .

Barry F ， Chai F ， Chijcheapaza-Flores H ， et al . Comparison of chemical-induced temporomandibular osteoarthritis rat models （monosodium iodoacetate versus collagenase type Ⅱ） for the study of prolonged drug delivery systems [J ] . PLoS One ， 2023 ， 18 （ 1 ）： e0281135 .

Wang GYF ， Shi XQ ， Wu WJ ， et al . Sustained and repeated mouth opening leads to development of painful temporomandibular disorders involving macrophage/microglia activation in mice [J ] . Pain ， 2018 ， 159 （ 7 ）： 1277 - 1288 .

Yokota H ， Leong DJ ， Sun HB . Mechanical loading：bone remodeling and cartilage maintenance [J ] . Curr Osteoporos Rep ， 2011 ， 9 （ 4 ）： 237 - 242 .

Utreja A ， Dyment NA ， Yadav S ， et al . Cell and matrix response of temporomandibular cartilage to mechanical loading [J ] . Osteoarthritis Cartilage ， 2016 ， 24 （ 2 ）： 335 - 344 .

Zhen GH ， Guo QY ， Li YS ， et al . Mechanical stress determines the configuration of TGFβ activation in articular cartilage [J ] . Nat Commun ， 2021 ， 12 ： 1706 .

DuFort CC ， Paszek MJ ， Weaver VM . Balancing forces：architectural control of mechanotransduction [J ] . Nat Rev Mol Cell Biol ， 2011 ， 12 （ 5 ）： 308 - 319 .

Heinegård D ， Saxne T . The role of the cartilage matrix in osteoarthritis [J ] . Nat Rev Rheumatol ， 2011 ， 7 （ 1 ）： 50 - 56 .

Loeser RF ， Goldring SR ， Scanzello CR ， et al . Osteoarthritis：a disease of the joint as an organ [J ] . Arthritis Rheum ， 2012 ， 64 （ 6 ）： 1697 - 1707 .

Stanton H ， Rogerson FM ， East CJ ， et al . ADAMTS5 is the major aggrecanase in mouse cartilage in vivo and in vitro [J ] . Nature ， 2005 ， 434 （ 7033 ）： 648 - 652 .

Glasson SS ， Askew R ， Sheppard B ， et al . Deletion of active ADAMTS5 prevents cartilage degradation in a murine model of osteoarthritis [J ] . Nature ， 2005 ， 434 （ 7033 ）： 644 - 648 .

Wang GW ， Wang MQ ， Wang XJ ， et al . Changes in the expression of MMP-3，MMP-9，TIMP-1 and aggrecan in the condylar cartilage of rats induced by experimentally created disordered occlusion [J ] . Arch Oral Biol ， 2010 ， 55 （ 11 ）： 887 - 895 .

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Related Institution

Department of Stomatology，Jinan Military General Hospital

Shandong Provincial Key Laboratory of Oral Tissue Regeneration，School of Stomatology of Shandong University

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