Predictive analysis of miR-34a-5p expression in pancreatic cancer tissue on poor prognosis

DONG Li-jun; LI Jie; CHAI Dou-dou; MOU Hong-chao

doi:10.11659/jjssx.12E024022

您当前的位置：

首页 >

文章列表页 >

Predictive analysis of miR-34a-5p expression in pancreatic cancer tissue on poor prognosis

Clinical Research | 更新时间：2025-09-24

- Predictive analysis of miR-34a-5p expression in pancreatic cancer tissue on poor prognosis
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 34, Issue 9, Pages: 817-821(2025)
- 作者机构：
  
  山东大学附属威海市立医院肝胆外科，山东威海 264200
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.12E024022
  CLC： R735.9
- Received：04 December 2024，
  
  Published：25 September 2025
- 稿件说明：
移动端阅览
董立军,李洁,柴豆豆等.胰腺癌组织中miR-34a-5p表达对预后不良的预测分析[J].局解手术学杂志,2025,34(09):817-821.

DONG Li-jun,LI Jie,CHAI Dou-dou,et al.Predictive analysis of miR-34a-5p expression in pancreatic cancer tissue on poor prognosis[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(09):817-821.
董立军,李洁,柴豆豆等.胰腺癌组织中miR-34a-5p表达对预后不良的预测分析[J].局解手术学杂志,2025,34(09):817-821. DOI： 10.11659/jjssx.12E024022.

DONG Li-jun,LI Jie,CHAI Dou-dou,et al.Predictive analysis of miR-34a-5p expression in pancreatic cancer tissue on poor prognosis[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(09):817-821. DOI： 10.11659/jjssx.12E024022.

摘要

目的

分析胰腺癌组织中微小RNA-34a-5p（miR-34a-5p）表达对患者术后预后不良的预测价值。

方法

收集123例胰腺癌患者手术切除的胰腺癌组织和癌旁正常组织，检测胰腺癌组织和癌旁正常组织中miR-34a-5p表达。比较不同临床病理特征患者胰腺癌组织中miR-34a-5p的表达。根据预后情况将患者分为预后不良组和预后良好组，并比较2组患者临床资料。采用

Cox

回归模型分析胰腺癌患者预后不良的危险因素，绘制受试者工作特征（

ROC

）曲线分析胰腺癌组织中miR-34a-5p表达对患者预后不良的预测价值。

结果

胰腺癌组织中miR-34a-5p表达低于癌旁正常组织（

0.05）。分化程度、TNM分期、淋巴结转移情况不同的患者胰腺癌组织中miR-34a-5p表达差异有统计学意义（

0.05）。预后不良组患者低分化、TNM分期Ⅲ期、淋巴结转移、切缘R1占比和糖类抗原199（CA199）、中性粒细胞与淋巴细胞比值（NLR）、血小板/淋巴细胞比值（PLR）水平均高于预后良好组（

0.05），miR-34a-5p表达低于预后良好组（

0.05）。

Cox

回归分析显示，低分化、TNM分期Ⅲ期、淋巴结转移、切缘R1、miR-34a-5p表达降低和CA199、NLR、PLR水平升高均是患者预后不良的危险因素（

0.05）；

ROC

曲线分析显示，胰腺癌组织中miR-34a-5p表达预测胰腺癌患者预后不良的最佳临界值为0.48，敏感度为78.82%，特异度为89.47%，曲线下面积为0.855，具有良好预测价值。

结论

胰腺癌组织中miR-34a-5p表达低于癌旁正常组织，且其表达与分化程度、TNM分期、淋巴结转移有关，亦是胰腺癌患者术后预后不良的危险因素和预测因子。

Abstract

Objective

To analyze the predictive value of microRNA-34a-5p （miR-34a-5p） expression in pancreatic cancer tissue on postoperative poor prognosis.

Methods

The surgically resected pancreatic cancer tissues and normal tissues adjacent to cancer from 123 patients with pancreatic cancer were collected to detect the expression of miR-34a-5p. The expression of miR-34a-5p in pancreatic cancer tissues for patients with different clinicopathological characteristics were compared. The patients were divided into the poor prognosis group and the g

ood prognosis group according to their prognosis，and the clinical data of patients between the two groups was compared. The risk factors of poor prognosis for patients with pancreatic cancer were analyzed by

Cox

regression model，and the predictive value of miR-34a-5p expression in pancreatic cancer tissues on poor prognosis of patients was analyzed by

receiver operating characteristic

（

ROC

） curve.

Results

The expression of miR-34a-5p in pancreatic cancer tissues was lower than that in normal tissues adjacent to cancer （

0.05）. There were statistically significant differences in the expression of miR-34a-5p in pancreatic cancer tissues of patients with different differentiation degrees，TNM stages，and lymph node metastasis （

0.05）. The proportions of low differentiation，TNM stage for stage Ⅲ，lymph node metastasis and incisal margin of R1，and levels of carbohydrate antigen 199 （CA199），neutrophils/lymphocytes ratio （NLR） and platelet/lymphocyte ratio （PLR） for patients in the poor prognosis group were higher than those in the good prognosis group （

0.05），while miR-34a-5p expression was lower than that in the good prognosis group （

0.05）.

Cox

regression analysis showed that low differentiation，TNM stage for stage Ⅲ，lymph node metastasis，incisal margin of R1，decreased expression of miR-34a-5p and increased levels of CA199，NLR and PLR were risk factors of poor prognosis for patients with pancreatic cancer（

0.05）.

ROC

curve analysis showed that the optimal cut-off value of miR-34a-5p expression in pancreatic cancer tissue for predicting poor prognosis of patients was 0.48，the sensitivity was 78.82%，the specificity was 89.47% and the area under the curve was 0.855，with good predictive value.

Conclusion

The expression of miR-34a-5p in pancreatic cancer tissue is lower than that in normal tissue adjacent to cancer，and its expression is related to the differentiation degree，TNM stage and lymph node metastasis，which is also a risk factor and predictor of poor prognosis for patients with pancreatic cancer.

关键词

Keywords

references

陈宏森，杨之雨，蒋栋铭，等 . 2006—2020年中国20岁及以上人群胰腺癌死亡分析及趋势预测［J］. 中华肿瘤防治杂志， 2023 ， 30 （ 12 ）： 699 - 707 . doi： 10.16073/j.cnki.cjcpt.2023.12.01 http://dx.doi.org/10.16073/j.cnki.cjcpt.2023.12.01 .

Olakowski M ， Grudzińska E . Pancreatic head cancer-current surgery techniques ［J］. Asian J Surg ， 2023 ， 46 （ 1 ）： 73 - 81 . doi： 10.1016/j.asjsur.2022.05.117 http://dx.doi.org/10.1016/j.asjsur.2022.05.117 .

李承圣，包绮晗，郝晓燕，等 . 基于随机森林算法的胰腺癌术后预测模型构建［J］. 吉林大学学报（医学版）， 2022 ， 48 （ 2 ）： 426 - 435 . doi： 10.13481/j.1671-587X.20220220 http://dx.doi.org/10.13481/j.1671-587X.20220220 .

Chu X ， Wei D ， Liu X ， et al . MicroRNAs as potential therapeutic targets for pancreatic cancer ［J］. Chin Med J （Engl）， 2022 ， 135 （ 1 ）： 4 - 10 . doi： 10.1097/CM9.00000000000001826 http://dx.doi.org/10.1097/CM9.00000000000001826 .

Kawai M ， Fukuda A ， Otomo R ， et al . Early detection of pancreatic cancer by comprehensive serum miRNA sequencing with automated machine learning ［J］. Br J Cancer ， 2024 ， 131 （ 7 ）： 1158 - 1168 . doi： 10.1038/s41416-024-02794-5 http://dx.doi.org/10.1038/s41416-024-02794-5 .

蒋莉萍，王霞，彭心华 . miR-34a-5p通过靶向IMP3、PD-L1对乳腺癌细胞生物学行为的影响［J］. 中国比较医学杂志， 2023 ， 33 （ 7 ）： 67 - 77 . doi： 10.3969/j.issn.1671-7856.2023.07.009 http://dx.doi.org/10.3969/j.issn.1671-7856.2023.07.009 .

He T ， Huang J ， Liang H ， et al . Circular RNA hsa_circ_0005239 contributes to hepatocellular carcinoma cell migration，invasion，and angiogenesis by targeting the miR-34a-5p/PD-L1 axis ［J］. Cell Biol Int ， 2023 ， 47 （ 9 ）： 1519 - 1534 . doi： 10.1002/cbin.12049 http://dx.doi.org/10.1002/cbin.12049 .

Chen S ， Yuan M ， Chen H ， et al . MiR-34a-5p suppresses cutaneous squamous cell carcinoma progression by targeting SIRT6 ［J］. Arch Dermatol Res ， 2024 ， 316 （ 6 ）： 299 . doi： 10.1007/s00403-024-03106-w http://dx.doi.org/10.1007/s00403-024-03106-w .

赵丽莉，贾雯媛，韩天奇，等 . miR-34a-5p介导上皮-间充质转化促进胰腺癌细胞株PANC-1的侵袭转移及化疗抵抗研究［J］. 中国临床药理学杂志， 2024 ， 40 （ 8 ）： 1155 - 1159 . doi： 10.13699/j.cnki.1001-6821.2024.08.014 http://dx.doi.org/10.13699/j.cnki.1001-6821.2024.08.014 .

中国抗癌协会胰腺癌专业委员会 . 胰腺癌综合诊治指南（2018版）［J］. 临床肝胆病杂志， 2018 ， 34 （ 10 ）： 2109 - 2120 . doi： 10.3969/j.issn.1001-5256.2018.10.011 http://dx.doi.org/10.3969/j.issn.1001-5256.2018.10.011 .

Wood LD ， Canto MI ， Jaffee EM ， et al . Pancreatic cancer： pathogenesis，screening，diagnosis，and treatment ［J］. Gastroenterology ， 2022 ， 163 （ 2 ）： 386 - 402 . doi： 10.1053/j.gastro.2022.03.056 http://dx.doi.org/10.1053/j.gastro.2022.03.056 .

Liu C ， An L ， Zhang S ， et al . Association between preoperative sarcopenia and prognosis of pancreatic cancer after curative-intent surgery： a updated systematic review and meta-analysis ［J］. World J Surg Oncol ， 2024 ， 22 （ 1 ）： 38 . doi： 10.1186/s12957-024-03310-y http://dx.doi.org/10.1186/s12957-024-03310-y .

Dittmar RL ， Liu S ， Tai MC ， et al . Plasma miRNA biomarkers in limited volume samples for detection of early-stage pancreatic cancer ［J］. Cancer Prev Res （Phila）， 2021 ， 14 （ 7 ）： 729 - 740 . doi： 10.1158/ 1940-6207.CAPR-20-0303 http://dx.doi.org/10.1158/1940-6207.CAPR-20-0303 .

Lin Z ， Chen Y ， Lin Y ， et al . Potential miRNA biomarkers for the diagnosis and prognosis of esophageal cancer detected by a novel absolute quantitative RT-qPCR method ［J］. Sci Rep ， 2020 ， 10 （ 1 ）： 20065 . doi： 10.1038/s41598-020-77119-6 http://dx.doi.org/10.1038/s41598-020-77119-6 .

邓思雨，梁冰，魏薇，等 . miR-34a-5p对三阴性乳腺癌细胞的影响及相关机制研究［J］. 中国生物工程杂志， 2023 ， 43 （ 1 ）： 18 - 26 . doi： 10.13523/j.cb.2206036 http://dx.doi.org/10.13523/j.cb.2206036 .

金玲玲，喻志英，朱进璐 . 宫颈癌患者血清miR-329-3p和miR-34a-5p水平检测及临床意义［J］. 中国妇幼保健， 2019 ， 34 （ 22 ）： 5301 - 5304 . doi： 10.7620/zgfybj.j.issn.1001-4411.2019.22.74 http://dx.doi.org/10.7620/zgfybj.j.issn.1001-4411.2019.22.74 .

葛新国，原小斌 . 细胞分裂周期蛋白20、微小RNA-34a-5p在膀胱尿路上皮癌组织中的表达及与临床病理的相关性［J］. 临床外科杂志， 2021 ， 29 （ 11 ）： 1075 - 1078 . doi： 10.3969/j.issn.1005-6483.2021.11.023 http://dx.doi.org/10.3969/j.issn.1005-6483.2021.11.023 .

陈娟，尤卞红 . 高危人乳头状瘤病毒感染宫颈癌患者miR-34a-5p表达及意义［J］. 肿瘤学杂志， 2020 ， 26 （ 11 ）： 977 - 980 . doi： 10.11735/j.issn.1671-170X.2020.11.B010 http://dx.doi.org/10.11735/j.issn.1671-170X.2020.11.B010 .

Hasakova K ， Reis R ， Vician M ， et al . Expression of miR-34a-5p is up-regulated in human colorectal cancer and correlates with survival and clock gene PER2 expression ［J］. PLoS One ， 2019 ， 14 （ 10 ）： e0224396 . doi： 10.1371/journal.pone.0224396 http://dx.doi.org/10.1371/journal.pone.0224396 .

Sun Z ， Zhang B ， Cui T . Long non-coding RNA XIST exerts oncogenic functions in pancreatic cancer via miR-34a-5p ［J］. Oncol Rep ， 2018 ， 39 （ 4 ）： 1591 - 1600 . doi： 10.3892/or.2018.6245 http://dx.doi.org/10.3892/or.2018.6245 .

钱佳璐，汤娜 . 血小板与淋巴细胞比值联合CA19-9预测胰腺癌预后［J］. 现代消化及介入诊疗， 2022 ， 27 （ 4 ）： 512 - 515 . doi： 10.3969/j.issn.1672-2159.2022.04.024 http://dx.doi.org/10.3969/j.issn.1672-2159.2022.04.024 .

徐凤，王汉譞，马右维，等 . 术前cPNI对交界可切除胰腺癌患者术后远期预后的影响［J］. 中华肝胆外科杂志， 2024 ， 30 （ 7 ）： 520 - 524 . doi： 10.3760/cma.j.cn113884-20240430-00130 http://dx.doi.org/10.3760/cma.j.cn113884-20240430-00130 .

Homolová J ， Ondruš D ， Ondrušová M ， et al . Pancreatic cancer-epidemiology，risk factors，nutritional and inflammatory prognostic and predictive factors ［J］. Klin Onkol ， 2024 ， 38 （ 4 ）： 270 - 276 . doi： 10.48095/ccko2024270 http://dx.doi.org/10.48095/ccko2024270 .

安革利，董峰，宋利芳 . 胰腺癌根治术后患者预后的相关影响因素［J］. 实用癌症杂志， 2023 ， 38 （ 1 ）： 126 - 128 . doi： 10.3969/j.issn.1001-5930.2023.01.036 http://dx.doi.org/10.3969/j.issn.1001-5930.2023.01.036 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Expression of miR-205 and miR-367 in cutaneous malignant melanoma tissue and their clinical significances

Effect of endoscopic surgery combined with postoperative nasal irrigation on symptoms improvement and prognosis in patients with allergic fungal sinusitis

Correlation between serum ferritin and response rate and prognosis of lung cancer immunotherapy

Influencing factors of liver regeneration after laparoscopic anatomic liver resection and its relationship with prognosis

Clinical effects of different bone graft materials in internal fixation surgery for Schatzker type Ⅱ tibial plateau fracture

Related Author

ZHANG Li-jun

YAN Wei-heng

HUANG Guo-qing

SUN Chong-zhou

ZHU Liming

NA Xuewu

LI Haizhou

SUN Jianhua

Related Institution

Department of Burns and Plastic Surgery， Mianyang Hospital， Medical College of University of Electronic Science and Technology of China/Mianyang Central Hospital

Department of ENT, Jinzhou Central Hospital

Department of Medical Laboratory，the First Affiliated Hospital of Army Medical University

Department of Hepatobiliary and Vascular Surgery，People's Hospital Affiliated to Shandong First Medical University

Department of Endocrinology and Metabolic Diseases，People's Hospital Affiliated to Shandong First Medical University

⁰