Effect of exogenous luteinizing hormone on vitrification granulosa cells and its mechanism

CHEN Jie; GAO Shang; ZHANG Shao-jie; YANG Xi; LI Kun; SHI Jun; WANG Xing

doi:10.11659/jjssx.12E023006

您当前的位置：

首页 >

文章列表页 >

Effect of exogenous luteinizing hormone on vitrification granulosa cells and its mechanism

Basic Research | 更新时间：2025-01-16

- Effect of exogenous luteinizing hormone on vitrification granulosa cells and its mechanism
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 34, Issue 1, Pages: 1-4(2025)
- 作者机构：
  
  1.内蒙古医科大学基础医学院人体解剖学教研室，内蒙古呼和浩特 010110
  2.内蒙古医科大学干细胞研究中心，内蒙古呼和浩特 010110
  3.内蒙古医科大学基础医学院数字医学中心，内蒙古呼和浩特 010110
  4.内蒙古医科大学基础医学院生理学教研室，内蒙古呼和浩特 010110
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.12E023006
  CLC： R711.75
- Published：25 January 2025，
  
  Received：02 December 2023，
- 稿件说明：
移动端阅览
CHEN JIE, GAO SHANG, ZHANG SHAO-JIE, et al. Effect of exogenous luteinizing hormone on vitrification granulosa cells and its mechanism. [J]. Journal of regional anatomy and operative surgery, 2025, 34(1): 1-4.
DOI：

CHEN JIE, GAO SHANG, ZHANG SHAO-JIE, et al. Effect of exogenous luteinizing hormone on vitrification granulosa cells and its mechanism. [J]. Journal of regional anatomy and operative surgery, 2025, 34(1): 1-4. DOI： 10.11659/jjssx.12E023006.

摘要

目的

观察外源性黄体生成素（LH）对玻璃化冻存颗粒细胞的影响及其作用机制。

方法

取4周龄青春期前小鼠颗粒细胞，分为新鲜组（不进行玻璃化冻存）、玻璃化冻存组（按玻璃化冻存程序处理）和LH组（在玻璃化冻存组的基础上添加0.3 IU/mL LH干预）。进行颗粒细胞计数，采用Realtime PCR及Western blot检测各组Foxl2、PI3K、AKT及mTOR mRNA和蛋白的表达变化。

结果

与玻璃化冻存组相比，LH组颗粒细胞总数显著增加（

0.05），Foxl2、PI3K、AKT、mTOR mRNA及蛋白的表达显著升高（

0.05）。

结论

外源性LH干预有利于冻存颗粒细胞的存活，其机制可能与激活PI3K/AKT/mTOR通路有关。

Abstract

Objective

To investigate the effect of exogenous luteinizing hormone （LH） on vitrification granulosa cells and its mechanisms.

Methods

The granulosa cells of 4-week-old preadolescent mice were selected and divided into the fresh group （without vitrification procedure）， the vitrification group （treated according to vitrification procedure） and the LH group （treated with 0.3 IU/L of LH intervention on the basis of the vitrification group）. Granulocyte count was performed， and Realtime PCR and Western blot were used to detect the expression changes of Foxl2， PI3K， AKT and mTOR mRNAs and proteins in each group.

Results

Compared with the vitrification group，the total number of granulosa cells in the LH group was significantly increased （

0.05），and the expression of Foxl2，PI3K，AKT and mTOR mRNAs and proteins were elevated （

0.05）.

Conclusion

The intevention of exogenous LH is beneficial for the survival of vitrification granulosa cells， and the mechanism may be related to the activation of PI3K/AKT/mTOR pathway.

关键词

颗粒细胞玻璃化冻存黄体生成素Foxl2PI3K/AKT/mTOR通路

Keywords

granulosa cellsvitrificationluteinizing hormoneFoxl2PI3K/AKT/mTOR pathway

references

Cariati F，Carbone L，Iorio GG，et al.Cryopreservation of ovarian tissue：the biggest challenge of oncofertility［J］.Minerva Obstet Gynecol，2023，75（4）：371-378.doi：10.23736/S2724-606X.22.05066-7http://dx.doi.org/10.23736/S2724-606X.22.05066-7.

张旭红，陈杰.小鼠卵巢体外培养过程中FSH干预对VEGF及其受体表达的影响［J］.局解手术学杂志，2020，29（12）：950-953.doi： 10.11659/jjssx.04E020086http://dx.doi.org/10.11659/jjssx.04E020086.

Fabová Z，Loncová B，Sirotkin AV.MicroRNA miR-125b can suppress ovarian granulosa cell functions：interrelationships with FSH［J］.Cell Biochem Funct，2023，41（2）：177-188.doi：10.1002/cbf.3771http://dx.doi.org/10.1002/cbf.3771.

Qi Y，Xue HL，Zheng YJ，et al.The photoperiod regulates granulosa cell apoptosis through the FSH-Nodal/ALK7 signaling pathway in phodopus sungorus［J］.Animals （Basel），2022，12（24）：3570.doi：10.3390/ani12243570http://dx.doi.org/10.3390/ani12243570.

He Z，Yuan B.Diagnostic value of combined detection of pelvic ultrasound and serum LH，FSH，and E2 levels in children with idiopathic central precocious puberty［J］.Evid Based Complement Alternat Med，2022，2022：7928344.doi：10.1155/2022/7928344http://dx.doi.org/10.1155/2022/7928344.

王仕斌，王雪婷，薛蓉亮.右美托咪啶对人宫颈癌细胞Hela生长和转移能力的影响［J］.局解手术学杂志，2018，27（12）：854-858.doi： 10.11659/jjssx.03E018127http://dx.doi.org/10.11659/jjssx.03E018127.

Tang Y，Lin Z，Liu L，et al.Attenuated AKT signaling by miR-146a-5p interferes with chicken granulosa cell proliferation， lipid deposition and progesterone biosynthesis［J］.Theriogenology，2024，214：370-385.doi：10.1016/j.theriogenology.2023.11.007http://dx.doi.org/10.1016/j.theriogenology.2023.11.007.

Martínez-Peña AA，Petrik JJ，Hardy DB，et al.Delta-9-tetrahydrocannabinol increases vascular endothelial growth factor （VEGF） secretion through a cyclooxygenase-dependent mechanism in rat granulosa cells［J］.Reprod Toxicol，2022，111：59-67.doi：10.1016/j.reprotox. 2022.05.004http://dx.doi.org/10.1016/j.reprotox.2022.05.004.

Irusta G，Abramovich D，Parborell F，et al.Direct survival role of vascular endothelial growth factor （VEGF） on rat ovarian follicular cells［J］.Mol Cell Endocrinol，2010，25（1/2）：93-100.doi：10.1016/j.mce.2010.04.018http://dx.doi.org/10.1016/j.mce.2010.04.018.

Di Nisio V，Rossi G，Iorio R，et al.VEGFR2 expression is differently modulated by parity and nulliparity in mouse ovary［J］.Biomed Res Int， 2018，2018：6319414.doi：10.1155/2018/6319414http://dx.doi.org/10.1155/2018/6319414.

Liu C，Peng J，Matzuk MM，et al.Lineage specification of ovarian theca cells requires multicellular interactions via oocyte and granulosa cells［J］.Nat Commun，2015，6：6934.doi：10.1038/ncomms7934http://dx.doi.org/10.1038/ncomms7934.

Bird AD，Frost ER，Bagheri-Fam S，et al.Somatic FGFR2 is required for germ cell maintenance in the mouse ovary［J］.Endocrinology，2023，164（5）：bqad031.doi：10.1210/endocr/bqad031http://dx.doi.org/10.1210/endocr/bqad031.

Diaz AA，Kubo H，Handa N，et al.A systematic review of ovarian tissue transplantation outcomes by ovarian tissue processing size for cryopreservation［J］.Front Endocrinol （Lausanne），2022，13：918899.doi：10.3389/fendo.2022.918899http://dx.doi.org/10.3389/fendo.2022.918899.

Del Castillo LM，Buigues A，Rossi V，et al.The cyto-protective effects of LH on ovarian reserve and female fertility during exposure to gonadotoxic alkylating agents in an adult mouse model［J］.Hum Reprod，2021，36（9）：2514-2528.doi：10.1093/humrep/deab165http://dx.doi.org/10.1093/humrep/deab165.

De Bortoli MA，Garraza MH，Aguado LI.Epinephrine intracerebroventricular stimulation modifies the LH effect on ovarian progesterone and androstenedione release［J］.J Steroid Biochem Mol Biol，2000，74（1/2）：19-24.doi：10.1016/s0960-0760（00）00084-4http://dx.doi.org/10.1016/s0960-0760（00）00084-4.

Zheng X， Chen J， Yang Y， et al. Exogenous luteinizing hormone promotes ovarian survival and function during cryopreservation and transplantation［J］.Biochem Biophys Res Commun， 2020， 526（2）：424-430. doi： 10.1016/j.bbrc.2020.03.014http://dx.doi.org/10.1016/j.bbrc.2020.03.014.

Kuokkanen S，Pal L.Follicle-stimulating hormone （and luteinizing hormone） in ovarian stimulation：does the dose matter for cycle success？［J］.Fertil Steril，2023，119（2）：166-169.doi：10.1016/j.fertnstert.2022.12.019http://dx.doi.org/10.1016/j.fertnstert.2022.12.019.

Ali EMT，Abdallah HI，El-Sayed SM.Histomorphological，VEGF and TGF-β immunoexpression changes in the diabetic rats' ovary and the potential amelioration following treatment with metformin and insulin［J］.J Mol Histol，2020，51（3）：287-305.doi：10.1007/s10735-020-09880-xhttp://dx.doi.org/10.1007/s10735-020-09880-x.

Kılınç Uğurlu A，Bideci A，Demirel MA，et al.Effects of blue light on puberty and ovary in female rats［J］.J Clin Res Pediatr Endocrinol，2023，15（4）：365-374.doi：10.4274/jcrpe.galenos.2023.2022-12-1http://dx.doi.org/10.4274/jcrpe.galenos.2023.2022-12-1.

Zhao X，Li H，Chen X，et al.Long non-coding RNA MSTRG.5970.28 regulates proliferation and apoptosis of goose follicle granulosa cells via the miR-133a-3p/ANOS1 pathway［J］.Poult Sci，2023，102（3）：102451.doi：10.1016/j.psj.2022.102451http://dx.doi.org/10.1016/j.psj.2022.102451.

Zhang H，Luo Q，Lu X，et al.Retraction note：effects of hPMSCs on granulosa cell apoptosis and AMH expression and their role in the restoration of ovary function in premature ovarian failure mice［J］.Stem Cell Res Ther，2022，13（1）：504.doi：10.1186/s13287-022-03183-6http://dx.doi.org/10.1186/s13287-022-03183-6.

Xiao L，Wang Z，Lu N，et al.GPER mediates the IL6/JAK2/STAT3 pathway involved in VEGF expression in swine ovary GCs［J］.J Mol Endocrinol，2021，68（1）：23-33.doi：10.1530/JME-21-0125http://dx.doi.org/10.1530/JME-21-0125.

Jin L，Ren L，Lu J，et al.CXCL12 and its receptors regulate granulosa cell apoptosis in PCOS rats and human KGN tumor cells［J］.Reproduction，2021，161（2）：145-157.doi：10.1530/REP-20-0451http://dx.doi.org/10.1530/REP-20-0451.

Sperduti S，Paradiso E，Anzivino C，et al.LH increases the response to FSH in granulosa-lutein cells from sub/poor-responder patients in vitro［J］.Hum Reprod，2023，38（1）：103-112.doi：10.1093/humrep/deac246http://dx.doi.org/10.1093/humrep/deac246.

Edmund LN，Salama AM，Murali R.Cytologic features of sex cord-stromal tumors in women［J］.Cancer Cytopathol，2022，130（1）：55-71.doi：10.1002/cncy.22502http://dx.doi.org/10.1002/cncy.22502.

Li XF，Wang QF，He QQ，et al.Low LH level does not indicate poor IVF cycle outcomes with GnRh-a single trigger：a retrospective analysis［J］.BMC Pregnancy Childbirth，2022，22（1）：951.doi：10.1186/s12884-022-05251-4http://dx.doi.org/10.1186/s12884-022-05251-4.

范培，李庆云，何庭月，等.补肾益冲方对卵巢储备功能减退大鼠卵巢组织PI3K/Akt/mTOR信号通路的影响［J］.江西中医药，2024，55（10）：64-68.doi：10.20141/j.0411-9584.2024.10.19http://dx.doi.org/10.20141/j.0411-9584.2024.10.19.

Hojo T，Skarzynski DJ，Okuda K.Apoptosis，autophagic cell death，and necroptosis：different types of programmed cell death in bovine corpus luteum regression［J］.J Reprod Dev，2022，68（6）：355-360.doi：10.1262/jrd.2022-097http://dx.doi.org/10.1262/jrd.2022-097.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰