Protective effect and mechanism of propofol on acute kidney injury in septic rats

YU Pei; ZHANG Bin-dan

doi:10.11659/jjssx.11E024069

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Protective effect and mechanism of propofol on acute kidney injury in septic rats

Basic Research | 更新时间：2025-11-25

- Protective effect and mechanism of propofol on acute kidney injury in septic rats
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 34, Issue 11, Pages: 933-937(2025)
- 作者机构：
  
  陆军军医大学大坪医院麻醉科，重庆 400042
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.11E024069
  CLC： R692
- Received：13 November 2024，
  
  Published：25 November 2025
- 稿件说明：
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余沛,张濒丹.丙泊酚对脓毒症大鼠急性肾损伤的保护作用及机制[J].局解手术学杂志,2025,34(11):933-937.

YU Pei,ZHANG Bin-dan.Protective effect and mechanism of propofol on acute kidney injury in septic rats[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(11):933-937.
余沛,张濒丹.丙泊酚对脓毒症大鼠急性肾损伤的保护作用及机制[J].局解手术学杂志,2025,34(11):933-937. DOI： 10.11659/jjssx.11E024069.

YU Pei,ZHANG Bin-dan.Protective effect and mechanism of propofol on acute kidney injury in septic rats[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(11):933-937. DOI： 10.11659/jjssx.11E024069.

摘要

目的

探究丙泊酚对脓毒症大鼠急性肾损伤的保护作用及潜在机制。

方法

84只成年SD大鼠随机分为假手术组、模型组和治疗组。假手术组仅开关腹，模型组行盲肠结扎穿孔术建模。治疗组于建模前30 min腹腔注射丙泊酚（60 mg/kg），建模后6 h再次腹腔注射相同剂量丙泊酚。观察各组大鼠肾功能和肾病理改变，记录各组大鼠24 h内存活情况，并计算24 h存活率。通过脂多糖（LPS）刺激人肾小管上皮细胞系建立体外脓毒症模型，观察肾小管上皮细胞凋亡及氧化应激的变化；分析丙泊酚对LPS刺激后的肾小管上皮细胞氧化应激及凋亡的影响。

结果

脓毒症大鼠出现肾功能下降和肾组织结构破坏，大鼠24 h存活率显著下降，生存时间缩短。丙泊酚可有效改善脓毒症大鼠肾功能，降低肾Paller评分，提高大鼠存活率，延长大鼠生存时间。LPS可导致肾小管上皮细胞凋亡和细胞氧化应激，而丙泊酚可抑制肾小管上皮细胞氧化应激和细胞凋亡。

结论

丙泊酚可有效减轻脓毒症大鼠急性肾损伤，其机制与抑制肾小管上皮细胞凋亡和氧化应激有关。

Abstract

Objective

To explore the protective effect and potential mechanism of propofol on acute kidney injury in septic rats.

Methods

A total of 84 adult SD rats were randomly divided into the sham surgery group， the model group and the treatment group. The sham surgery group only opened and closed the abdomen， while the model group underwent cecal ligation and perforation for modeling. In the treatment group， propofol （60 mg/kg） was intraperitoneally injected 30 minutes before modeling， and the same dose of propofol was intraperitoneally injected again 6 hours after modeling. The renal function and pathological changes of rats in each group were observed， 24-hour survival status of rats in each group were recorded， and the 24-hour survival rate was calculated. A sepsis model was established in vitro by stimulating human renal tubular epithelial cell line with lipopolysaccharide （LPS）， and the changes of apoptosis and oxidative stress of renal tubular epithelial cells were observed. The effects of propofol on oxidative stress and apoptosis of renal tubular epithelial cells stimulated by LPS were analyzed.

Results

The renal function of sepsis rats was decreased， the renal tissue structure was damaged， the 24-hour survival rate of rats was decreased significantly and the survival time was shortened. Propofol could effectively improve renal function， reduce renal Paller score，increase survival rate and prolong survival time in sepsis rats. LPS could induce apoptosis and oxidative stress of renal tubular epithelial cells， while propofol could inhibit oxidative stress and apoptosis of renal tubular epithelial cells.

Conclusion

Propofol can effectively alleviate acute kidney injury in septic rats， and its mechanism is related to the inhibition of apoptosis and oxidative stress of renal tubular epithelial cells.

关键词

Keywords

references

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