Regulatory effects of miR-205 on proliferation,migration,and epithelial-mesenchymal transition in colon cancer cells

Maierdan· Sailimu; JIN Zhong; Alidan· Aierlken; KE Yu-bi

doi:10.11659/jjssx.10E024042

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Regulatory effects of miR-205 on proliferation,migration,and epithelial-mesenchymal transition in colon cancer cells

Basic Research | 更新时间：2025-07-31

- Regulatory effects of miR-205 on proliferation,migration,and epithelial-mesenchymal transition in colon cancer cells
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 34, Issue 8, Pages: 663-667(2025)
- 作者机构：
  
  1.新疆医科大学第一附属医院综合外科，新疆乌鲁木齐 830054
  2.新疆医科大学第一附属医院全科医学科，新疆乌鲁木齐 830054
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.10E024042
  CLC： R735.35
- Received：13 October 2024，
  
  Published：25 August 2025
- 稿件说明：
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买尔旦·赛力木 ,金钟,阿里旦·艾尔肯等.miR-205对结肠癌细胞增殖、迁移和上皮间质转化的调控作用研究[J].局解手术学杂志,2025,34(08):663-667.

Maierdan·Sailimu ,JIN Zhong,Alidan·Aierlken ,et al.Regulatory effects of miR-205 on proliferation,migration,and epithelial-mesenchymal transition in colon cancer cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(08):663-667.
买尔旦·赛力木 ,金钟,阿里旦·艾尔肯等.miR-205对结肠癌细胞增殖、迁移和上皮间质转化的调控作用研究[J].局解手术学杂志,2025,34(08):663-667. DOI： 10.11659/jjssx.10E024042.

Maierdan·Sailimu ,JIN Zhong,Alidan·Aierlken ,et al.Regulatory effects of miR-205 on proliferation,migration,and epithelial-mesenchymal transition in colon cancer cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(08):663-667. DOI： 10.11659/jjssx.10E024042.

摘要

目的

探讨microRNA-205（miR-205）在结肠癌中的表达及其对结肠癌细胞增殖、迁移和上皮间质转化（EMT）的调控作用。

方法

用qRT-PCR检测结肠癌组织、癌旁组织及结肠癌细胞HT-29中miR-205的表达水平。将HT-29细胞分为3组，转染miR-205模拟物的细胞为miR-205模拟物组，转染miR-205抑制物的细胞为miR-205抑制物组，仅用Lipo2000转染试剂处理的细胞作为Buffer组。采用CCK-8实验和EDU实验检测各组细胞的增殖活力；Transwell实验检测细胞的迁移能力；Western blot检测各组细胞EMT相关蛋白N-cadherin、Vimentin和E-cadherin的表达。

结果

与癌旁组织相比，结肠癌组织中miR-205的相对表达水平显著下调（

0.05）。与Buffer组相比，miR-205模拟物组细胞中miR-205的相对表达水平上调，miR-205抑制物组细胞中miR-205的相对表达水平下调，差异均有统计学意义（

0.05）。过表达miR-205显著抑制结肠癌细胞的增殖活力及迁移能力（

0.05），而抑制miR-205则促进结肠癌细胞的增殖活力及迁移能力（

0.05）。与Buffer组相比，miR-205模拟物组细胞中N-cadherin、Vimentin下调，E-cadherin上调，而miR-205抑制物组细胞中N-cadherin、Vimentin上调，E-cadherin下调，差异均有统计学意义（

0.05）。

结论

miR-205在结肠癌组织中的表达显著降低，并通过下调结肠癌细胞的增殖、迁移和EMT来发挥其抑癌作用。

Abstract

Objective

To investigate the expression of microRNA-205 （miR-205） in colon cancer and its regulatory effects on the proliferation，migration，and epithelial-mesenchymal transition （EMT） of colon cancer cells.

Methods

The expression levels of miR-205 in colon cancer tissues，adjacent normal tissues，and colon cancer cell HT-29 were detected by qRT-PCR. HT-29 cells were divided into three groups，cells transfected with miR-205 mimics were named the miR-205 mimic group，cells transfected with miR-205 inhibitors were named the miR-205 inhibitor group，and cells treated only with Lipo2000 transfection reagent were named the Buffer group.The proliferative activity of cells in each group was detected by CCK-8 assay and EDU assay；the migration invasion ability of cells was detected by Transwell assay； the expressions of EMT-related proteins N-cadherin，Vimentin，and E-cadherin in each group were detected by Western blot.

Results

Compared with adjacent normal tissues，the relative expression level of miR-205 in colon cancer tissues was significantly down-regulated （

0.05）. Compared with the Buffer group，the relative expression level of miR-205 in the cells of the miR-205 mimic group was up-regulated，while that in the miR-205 inhibitor group was down-regulated，the differences were statistically significant （

0.05）. Overexpression of miR-205 significantly inhibited the proliferation activity and migration ability of colon cancer cells （

0.05），while inhibition of miR-205 promoted the proliferation activity and migration ability of colon cancer cells （

0.05）. Compared with the Buffer group，the levels of N-cadherin and Vimentin were down-regulated and the level of E-cadherin was up-regulated in the cells of the miR-205 mimic group，while the levels of N-cadherin and Vimentin were up-regulated and the level of E-cadherin was down-regulated in the cells of the miR-205 inhibitor group，the differences were all statistically significant （

0.05）.

Conclusion

The expression of miR-205 in colon cancer tissues is significantly decreased，and miR-205 exerts its tumor-suppressive effects by downregulating the proliferation，migration，and EMT of colon cancer cells.

关键词

Keywords

references

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