Effect of PSMA gene expression on the biological behavior of prostate cancer cells and its mechanism

WANG Ru-meng; ZHANG Ning; LIU Yang; WANG Chao; JIA Jia-qi; QU Wen-hao; JIA Li-zhou

doi:10.11659/jjssx.10E024025

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Effect of PSMA gene expression on the biological behavior of prostate cancer cells and its mechanism

Basic Research | 更新时间：2025-11-25

- Effect of PSMA gene expression on the biological behavior of prostate cancer cells and its mechanism
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 34, Issue 11, Pages: 943-949(2025)
- 作者机构：
  
  1.巴彦淖尔市医院中心实验室，内蒙古巴彦淖尔 015000
  2.右江民族医学院基础医学院，广西百色533000
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.10E024025
  CLC： R737.25
- Received：11 October 2024，
  
  Published：25 November 2025
- 稿件说明：
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王如梦,张宁,刘阳等.PSMA基因表达对前列腺癌细胞生物学行为的影响及作用机制研究[J].局解手术学杂志,2025,34(11):943-949.

WANG Ru-meng,ZHANG Ning,LIU Yang,et al.Effect of PSMA gene expression on the biological behavior of prostate cancer cells and its mechanism[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(11):943-949.
王如梦,张宁,刘阳等.PSMA基因表达对前列腺癌细胞生物学行为的影响及作用机制研究[J].局解手术学杂志,2025,34(11):943-949. DOI： 10.11659/jjssx.10E024025.

WANG Ru-meng,ZHANG Ning,LIU Yang,et al.Effect of PSMA gene expression on the biological behavior of prostate cancer cells and its mechanism[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(11):943-949. DOI： 10.11659/jjssx.10E024025.

摘要

目的

探究PSMA基因对前列腺癌细胞生物学行为的影响及其分子机制。

方法

利用GEPIA数据库分析PSMA在前列腺癌中的表达及其与预后的关系。利用RT-qPCR和Western blot分别检测正常前列腺上皮细胞RWPE-2及前列腺癌细胞DU145、PC-3中PSMA的mRNA和蛋白表达量。用DU145细胞构建敲减细胞株，分别转染PSMA慢病毒敲减质粒及其阴性对照，作为sh PSMA组和sh NC组；用PC-3细胞构建过表达细胞株，分别转染PSMA慢病毒过表达质粒及其阴性对照，作为OE PSMA组和OE NC组；CCK-8实验、克隆形成实验检测细胞增殖能力；细胞划痕实验、Transwell实验检测细胞迁移和侵袭能力；流式细胞术检测细胞凋亡情况；Western blot检测细胞中PI3K、Akt、BAX、Bcl-2蛋白的表达。将前列腺癌细胞DU145细胞株接种于BALB/c裸鼠左侧腋下成瘤，观察瘤块大小，并测定肿瘤质量；HE染色评估损伤程度；免疫组化染色检测PSMA的表达。

结果

GEPIA数据库分析显示，PSMA基因在前列腺癌中高表达，并与前列腺癌预后相关。RT-qPCR和Western blot结果显示，与RWPE-2细胞相比，PC-3、DU145细胞中PSMA的mRNA和蛋白表达水平均升高（

0.01）。与sh NC组相比，sh PSMA组细胞增殖、迁移和侵袭能力降低（

0.05），而细胞凋亡率升高（

0.05）。与OE NC组相比，OE PSMA组细胞增殖、迁移和侵袭能力升高（

0.05），而细胞凋亡率降低（

0.05）。与sh NC组相比，sh PSMA组细胞中PI3K、Akt、Bcl-2蛋白表达降低（

0.05），BAX蛋白表达升高（

0.05）；与OE NC组相比，OE PSMA组细胞中PI3K、Akt、Bcl-2蛋白表达升高（

0.05），BAX蛋白表达降低（

0.05）。裸鼠前列腺癌皮下移植瘤结果显示，敲低PSMA后，裸鼠肿瘤质量减轻（

0.05），肿瘤细胞形状不规则，细胞核深染，PSMA表达呈弱阳性。

结论

PSMA基因可能通过PI3K/Akt信号通路参与调控前列腺癌细胞的增殖、侵袭、迁移及凋亡过程。

Abstract

Objective

To explore the effect of PSMA gene on the biological behavior of prostate cancer cells and its molecular mechanism.

Methods

The expression of PSMA in prostate cancer and its relationship with prognosis were analyzed based on the GEPIA database. The mRNA and protein expression levels of PSMA in normal prostate epithelial cell RWPE-2 and prostate cancer cells DU145 and PC-3 were detected by RT-qPCR and Western blot，respectively. DU145 cells were used to construct knockdown cell lines，which were transfected with PSMA lentiviral knockdown plasmid and its negative control，serving as the sh PSMA group and the sh NC group，respectively； PC-3 cells were used to construct overexpression cell lines，which were transfected with PSMA lentiviral overexpression plasmid and its negative control，serving as the OE PSMA group and the OE NC group. CCK-8 assay and clone formation assay were used to detect the cell proliferation ability，wound healing assay and Transwell assay were used to detect the cell migration and invasion abilities，flow cytometry was used to detect the cell apoptosis，and Western blot was used to detect the expression of PI3K，Akt，BAX and Bcl-2 proteins. Prostate cancer cells of DU145 cell line were inoculated into the left armpit of BALB/c nude mice to form tumors，the tumor size was observed，and the tumor weight was measured； HE staining was used to evaluate the degree of damage； and the expression of PSMA was detected by immunohistochemistry.

Results

GEPIA database analysis showed that PSMA gene was highly expressed in prostate cancer and was related to the prognosis of prostate cancer. The results of RT-qPCR and Western blot showed that the mRNA and protein expression levels of PSMA in PC-3 and DU145 cells were higher than those in RWPE-2 cell（

0.01）. Compared with the sh NC group，the sh PSMA group showed decreased cell proliferation，migration and invasion abilities （

0.05），and increased cell apoptosis rate （

0.05）. Compared with the OE NC group，the OE PSMA group demonstrated increased cell proliferation，migration and invasion abilities（

0.05），and decreased apoptosis rate （

0.05）. Compared with the sh NC group，the protein expression of PI3K，Akt and Bcl-2 in the sh PSMA group decreased （

0.05），and the protein expression of BAX increased （

0.05）. Compared with the OE NC group，the protein expression of PI3K，Akt and Bcl-2 in the OE PSMA group increased （

0.05），and the protein expression of BAX decreased （

0.05）. Results of subcutaneous transplantation of prostate cancer in nude mice showed that the tumor weight of nude mice decreased （

0.05），tumor cells exhibited irregular shapes，nuclei were deeply stained，and PSMA expression was we

akly positive，after knocking down the PSMA.

Conclusion

PSMA gene may participate in regulating the proliferation，invasion，migration，and apoptosis of prostate cancer cells through the PI3K/Akt signaling pathway.

关键词

Keywords

references

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