Study on the effect and mechanism of long non-coding RNA FGD5-AS1 on pituitary adenoma

HAN Xiao-zheng; REN Hong-bo; HE Long; SONG Zhi-yuan; NIU Guo-dong

doi:10.11659/jjssx.10E024001

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Study on the effect and mechanism of long non-coding RNA FGD5-AS1 on pituitary adenoma

Basic Research | 更新时间：2025-11-25

- Study on the effect and mechanism of long non-coding RNA FGD5-AS1 on pituitary adenoma
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 34, Issue 11, Pages: 938-942(2025)
- 作者机构：
  
  邯郸市中心医院神经外三科，河北邯郸 056008
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.10E024001
  CLC： R736.4
- Received：01 October 2024，
  
  Published：25 November 2025
- 稿件说明：
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韩晓正,任洪波,贺龙等.长链非编码RNA FGD5-AS1对垂体瘤的影响及其机制研究[J].局解手术学杂志,2025,34(11):938-942.

HAN Xiao-zheng,REN Hong-bo,HE Long,et al.Study on the effect and mechanism of long non-coding RNA FGD5-AS1 on pituitary adenoma[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(11):938-942.
韩晓正,任洪波,贺龙等.长链非编码RNA FGD5-AS1对垂体瘤的影响及其机制研究[J].局解手术学杂志,2025,34(11):938-942. DOI： 10.11659/jjssx.10E024001.

HAN Xiao-zheng,REN Hong-bo,HE Long,et al.Study on the effect and mechanism of long non-coding RNA FGD5-AS1 on pituitary adenoma[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(11):938-942. DOI： 10.11659/jjssx.10E024001.

摘要

目的

探讨长链非编码RNA FGD5-AS1对垂体瘤（PA）细胞增殖、迁移、侵袭的影响，并分析其可能的作用机制。

方法

体外培养人PA细胞系HPAs、RC-4BC、HP75和人星形胶质细胞系NHA，RT-PCR检测上述细胞系中FGD5-AS1和miR-15a的表达水平。取对数生长期的HP75细胞随机分为沉默组和阴性对照组，沉默组转染shRNA-FGD5-AS1，阴性对照组转染shRNA-NC，RT-PCR检测2组细胞中FGD5-AS1和miR-15a的表达水平，CCK-8实验、细胞划痕实验、Transwell实验检测2组细胞增殖、迁移及侵袭能力，Western blot检测2组细胞Wnt/β-catenin信号通路相关蛋白表达，双荧光素酶报告基因实验验证FGD5-AS1和miR-15a的靶向关系。

结果

与NHA细胞相比，HPAs、RC-4BC、HP75细胞中FGD5-AS1的表达水平显著升高（

0.05），而miR-15a的表达水平显著降低（

0.05）。与阴性对照组相比，沉默组HP75细胞中的FGD5-AS1表达水平降低（

0.05），miR-15a表达水平升高（

0.05），OD值下降（

0.05），细胞迁移及侵袭能力降低（

0.05），Wnt3a和β-catenin蛋白表达降低（

0.05）。FGD5-AS1能特异性结合miR-15a使细胞荧光素酶活性降低（

0.05）。

结论

FGD5-AS1在PA细胞中表达升高，沉默FGD5-AS1能够抑制PA细胞的增殖、迁移及侵袭，其机制与其对miR-15a的靶向调控有关。

Abstract

Objective

To investigate the effects of long non-coding RNA FGD5-AS1 on the proliferation，migration，and invasion of pituitary adenoma （PA） cells，and to analyze its potential mechanism of action.

Methods

Human PA cell lines HPAs，RC-4BC，HP75，and human astrocyte cell line NHA were cultured in vitro.The expression levels of FGD5-AS1 and miR-15a in the above cell lines were detected by RT-PCR.HP75 cells in the logarithmic growth phase were randomly divided into the silencing group and the negative control group.The silencing group was transfected with shRNA-FGD5-AS1，while the negative control group was transfected with shRNA-NC.The expression levels of FGD5-AS1 and miR-15a in the two groups of cells were detected by RT-PCR.The proliferation，migration and invasion abilities of the two groups of cells were determined by CCK-8 assay，wound healing assay，and Transwell assay.The expression of proteins related to the Wnt/β-catenin signaling pathway in the two groups of cells was detected by Western blot.The targeting relationship between FGD5-AS1 and miR-15a was verified by dual-luciferase reporter gene assay.

Results

Compared with the NHA cell，the expression level of FGD5-AS1 was significantly increased in the HPAs，RC-4BC，and HP75 cells （（

0.05），whereas the expression level of miR-15a was significantly decreased （

0.05）.Compared with the negative control group，the expression level of FGD5-AS1 was decreased （

0.05），the expression level of miR-15a was increased （

0.05），the OD value was decreased （

0.05），the migration and invasion abilities of cells were reduced （

0.05），and the expression of Wnt3a and β-catenin proteins was decreased in the silencing group of HP75 cells（

0.05）.FGD5-AS1 could specifically bind to miR-15a，leading to a decrease in cell luciferase activity （

0.05）.

Conclusion

FGD5-AS1 is overexpressed in PA cells，and silencing FGD5-AS1 can inhibit the proliferation，migration，and invasion of PA cells，and the mechanism is related to its targeted regulation of miR-15a.

关键词

Keywords

references

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