Effects of astaxanthin on proliferation, migration and apoptosis of non-small cell lung cancer cells

ZHU Ning-wei; YU Shu-fang; ZHAO Yan-yan; YAO Jia-qi; YING Jing-jing

doi:10.11659/jjssx.09E025050

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Effects of astaxanthin on proliferation, migration and apoptosis of non-small cell lung cancer cells

Basic Research | 更新时间：2026-01-19

- Effects of astaxanthin on proliferation, migration and apoptosis of non-small cell lung cancer cells
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 35, Issue 1, Pages: 18-22(2026)
- 作者机构：
  
  1.浙江药科职业大学药学院，浙江宁波 315100
  2.宁波市第二医院药学部，浙江宁波 315010
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.09E025050
  CLC： R284.1;R917
- Received：12 September 2025，
  
  Published：25 January 2026
- 稿件说明：
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朱宁伟,俞淑芳,赵嫣嫣等.虾青素对非小细胞肺癌细胞增殖、迁移和凋亡的影响[J].局解手术学杂志,2026,35(01):18-22.

ZHU Ning-wei,YU Shu-fang,ZHAO Yan-yan,et al.Effects of astaxanthin on proliferation, migration and apoptosis of non-small cell lung cancer cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(01):18-22.
朱宁伟,俞淑芳,赵嫣嫣等.虾青素对非小细胞肺癌细胞增殖、迁移和凋亡的影响[J].局解手术学杂志,2026,35(01):18-22. DOI： 10.11659/jjssx.09E025050.

ZHU Ning-wei,YU Shu-fang,ZHAO Yan-yan,et al.Effects of astaxanthin on proliferation, migration and apoptosis of non-small cell lung cancer cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(01):18-22. DOI： 10.11659/jjssx.09E025050.

摘要

目的

探究虾青素对非小细胞肺癌A549细胞增殖、迁移和凋亡的影响及可能的作用机制。

方法

采用不同浓度虾青素（1 mmol/L、2 mmol/L、4 mmol/L、6 mmol/L和8 mmol/L）干预A549细胞，采用CCK-8法检测不同浓度虾青素对非小细胞肺癌A549细胞增殖的影响；采用细胞划痕实验检测细胞迁移率；采用Hoechst33342染色和JC-1染色检测虾青素对非小细胞肺癌A549细胞凋亡的影响；采用Western blot检测凋亡相关蛋白NF-κB、B细胞淋巴瘤/白血病-2（Bcl-2）、Bcl-2相关X蛋白（Bax）、Caspase-3表达。

结果

虾青素能够抑制A549细胞增殖和迁移（

0.05），并促进细胞凋亡（

0.05）；虾青素能够下调A549细胞NF-κB和Bcl-2蛋白表达（

0.05），并上调Bax和Caspase-3蛋白表达（

0.05）。

结论

虾青素能够抑制A549细胞增殖和迁移，并通过NF-κB通路引起A549细胞凋亡。

Abstract

Objective

To investigate the effects of astaxanthin on proliferation， migration and apoptosis of non-small cell lung cancer A549 cells and the possible mechanism of action.

Methods

A549 cells were treated with various concentrations of astaxanthin （1 mmol/L， 2 mmol/L， 4 mmol/L， 6 mmol/L and 8 mmol/L）， and the effects of different concentrations of astaxanthin on the proliferation of non-small cell lung cancer A549 cells were assessed by the CCK-8 assay. The cell migration rate was detected by scratch wound healing assay. The effect of astaxanthin on apoptosis of non-small cell lung cancer A549 cells was detected by Hoechst33342 staining and JC-1 staining. The expression levels of apoptosis related proteins NF-κB，B cell lymphoma/leukemia-2（Bcl-2），Bcl-2-associated X protein（Bax）， Caspase-3 were detected by Western blot.

Results

Astaxanthin could inhibit the proliferation and migration of A549 cells（

0.05）， and promote cell apoptosis（

0.05）. Astaxanthin could down-regulate the expression levels of NF-κB and Bcl-2 proteins of A549 cells （

0.05）， and up-regulate the expression levels of Bax and Caspase-3 proteins（

0.05）.

Conclusion

Astaxanthin suppresses the proliferation and migration of A549 cells， and induce apoptosis of A549 cells via the NF-κB pathway.

关键词

Keywords

references

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