Mechanism of baicalin mediated inflammatory response in rats with inflammatory bowel disease

XIANG Yi-pin; JIAN Hui; TU Yan-hong

doi:10.11659/jjssx.07E024040

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Mechanism of baicalin mediated inflammatory response in rats with inflammatory bowel disease

Basic Research | 更新时间：2025-01-21

- Mechanism of baicalin mediated inflammatory response in rats with inflammatory bowel disease
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 34, Issue 2, Pages: 110-116(2025)
- 作者机构：
  
  1.武汉市第六医院中西医结合科，湖北武汉 430000
  2.武汉市第六医院胃肠外科，湖北武汉 430000
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.07E024040
  CLC： R285.5
- Published：25 February 2025，
  
  Received：08 July 2024，
- 稿件说明：
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XIANG YI-PIN, JIAN HUI, TU YAN-HONG. Mechanism of baicalin mediated inflammatory response in rats with inflammatory bowel disease. [J]. Journal of regional anatomy and operative surgery, 2025, 34(2): 110-116.
DOI：

XIANG YI-PIN, JIAN HUI, TU YAN-HONG. Mechanism of baicalin mediated inflammatory response in rats with inflammatory bowel disease. [J]. Journal of regional anatomy and operative surgery, 2025, 34(2): 110-116. DOI： 10.11659/jjssx.07E024040.

摘要

目的

探究黄芩苷通过微小RNA（miR-21）/信号转导子和转录激活子3（STAT3）信号通路介导炎症性肠病大鼠炎症反应的作用机制。

方法

选取60只SD大鼠采用三硝基苯磺酸（TNBS）结肠灌注法构建炎症性肠病大鼠模型，随机分为6组，每组10只，分别为模型组、低剂量组（灌胃20 mg/kg黄芩苷）、高剂量组（灌胃80 mg/kg黄芩苷）、高剂量+agomiR-NC组（灌胃80 mg/kg黄芩苷+尾静脉注射80 mg/kg miRNA阴性对照载体agomiR-NC）、高剂量+agomiR-21组（灌胃80 mg/kg黄芩苷+尾静脉注射80 mg/kg miR-21激动剂agomiR-21）、高剂量+agomiR-21+colivelin组（灌胃80 mg/kg黄芩苷+尾静脉注射80 mg/kg agomiR-21，同时腹腔注射1 mg/kg的STAT3激活剂colivelin）。另选取10只SD大鼠不作任何处理作为对照组。给药期间各组大鼠每2 d测量1次体质量，给药结束后观察各组大鼠状态；苏木精-伊红（HE）染色评估病理损伤；试剂盒检测髓过氧化物酶（MPO）活力、炎症因子及氧化应激指标；qRT-PCR检测miR-21、STAT3 mRNA水平；Western blot检测STAT3、p-STAT3蛋白表达。

结果

与模型组相比，低剂量组及高剂量组大鼠炎症性肠病状态及结肠组织病理损伤程度显著改善，白细胞介素-13（IL-13）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH）水平升高（

0.05），肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、丙二醛（MDA）、活性氧（ROS）水平及miR-21 mRNA、STAT3 mRNA、p-STAT3/STAT3蛋白表达水平降低（

0.05），且呈剂量依赖性。与高剂量+agomiR-NC组相比，高剂量+agomiR-21组大鼠炎症性肠病状态及结肠组织病理损伤程度加重，IL-13、SOD、GSH水平降低（

0.05），TNF-α、IL-6、MDA、ROS水平及miR-21 mRNA、STAT3 mRNA、p-STAT3/STAT3蛋白表达水平明显升高（

0.05）。与高剂量+agomiR-21组相比，高剂量+agomiR-21+colivelin组大鼠炎症性肠病状态及结肠组织病理损伤程度加重，IL-13、SOD、GSH水平降低（

0.05），TNF-α、IL-6、MDA、ROS水平及miR-21 mRNA、STAT3 mRNA、p-STAT3/STAT3蛋白表达水平明显升高（

0.05）。

结论

黄芩苷通过miR-21/STAT3信号通路降低炎症因子及氧化应激水平进而改善炎症性肠病大鼠结肠损伤。

Abstract

Objective

To explore the mechanism of baicalin mediating inflammatory response in rats with inflammatory bowel disease through microRNA （miR-21）/signal transducer and activator of transcription 3 （STAT3） signaling pathway.

Methods

A total of 60 SD rats were selected to construct inflammatory bowel disease rat model by trinitro-benzene-sulfonic acid（TNBS） colon perfusion method，and randomly divided into 6 groups，with 10 rats in each group. They were divided into model group，low-dose group （gavage of 20 mg/kg baicalin），high-dose group （gavage of 80 mg/kg baicalin），high-dose+agomiR-NC group （gavage of 80 mg/kg baicalin+tail vein injection of 80 mg/kg miRNA negative control vector agomiR-NC），high-dose+agomiR-21 group （gavage of 80 mg/kg baicalin+tail vein injection of 80 mg/kg miR-21 agonist agomiR-21） and high-dose+agomiR-21+colivelin group （gavage of 80 mg/kg baicalin+tail vein injection of 80 mg/kg agomiR-21，along with intraperitoneal injection of 1 mg/kg STAT3 activator colivelin）. Another 10 SD rats were selected as control group without any treatment. The body mass of rats in each group was measured every two days during administration，and the status of rats in each group was observed after administration. Hematoxylin and eosin（HE） staining was used to evaluate the pathological damage.The myeloperoxidase （MPO） activity，inflammatory factors and oxidative stress indexes were detected by the test kit. The mRNA levels of miR-21，STAT3 were detected by qRT-PCR. The expression of STAT3 and p-STAT3 protein was detected by Western blot.

Results

Compared with model group，the status of inflammatory bowel disease and pathological damage degree of colon tissue in low-dose group and high-dose group were significantly improved，the levels of interleukin-13 （IL-13），superoxide dismutase （SOD） and glutathione peroxidase （GSH） were increased （

0.05），while the levels of tumor necrosis factor-α （TNF-α），interleukin-6 （IL-6），malondialdehyde （MDA），reactive oxygen species （ROS），and miR-21 mRNA，STAT3 mRNA，p-STAT3/STAT3 protein expression were decrea

sed （

0.05） in a dose-dependent manner. Compared with high-dose+agomiR-NC group，the status of inflammatory bowel disease and pathological damage degree of colon tissue were aggravated in high-dose+agomiR-21 group，the levels of IL-13，SOD and GSH were decreased （

0.05），while the levels of TNF-α， IL-6，MDA，ROS，and miR-21 mRNA，STAT3 mRNA，P-STAT3/STAT3 protein expression were increased （

0.05）. Compared with high-dose+agomiR-NC group，the status of inflammatory bowel disease and pathological damage degree of colon tissue were aggravated in high-dose+agomiR-21+colivelin group，the levels of IL-13，SOD and GSH were decreased （

0.05），while the levels of TNF-α，IL-6，MDA，ROS，and miR-21 mRNA，STAT3 mRNA and P-STAT3 /STAT3 protein expression were increased （

0.05）.

Conclusion

Baicalin reduces inflammatory factors and oxidative stress levels through miR-21/STAT3 signaling pathway，and thereby ameliorates colonic injury in rats with inflammatory bowel disease.

关键词

炎症性肠病黄芩苷miR-21信号转导子和转录激活子3氧化应激炎症

Keywords

inflammatory bowel diseasebaicalinmiR-21signal transducer and activator of transcription 3oxidative stressinflammation

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