Experimental study on adenosine in promoting intestinal mucosal repair in ulcerative colitis

HUANG He; WANG Lei; WEN Xi-nian

doi:10.11659/jjssx.06E025025

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Experimental study on adenosine in promoting intestinal mucosal repair in ulcerative colitis

Digestive System Diseases | 更新时间：2026-03-17

- Experimental study on adenosine in promoting intestinal mucosal repair in ulcerative colitis
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 35, Issue 3, Pages: 199-204(2026)
- 作者机构：
  
  新疆医科大学第五附属医院胃肠外科，新疆乌鲁木齐 830011
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.06E025025
  CLC： R574.62
- Received：06 June 2025，
  
  Published：25 March 2026
- 稿件说明：
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黄河,王磊,文西年.腺苷促进溃疡性结肠炎肠黏膜修复的实验研究[J].局解手术学杂志,2026,35(03):199-204.

HUANG He,WANG Lei,WEN Xi-nian.Experimental study on adenosine in promoting intestinal mucosal repair in ulcerative colitis[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(03):199-204.
黄河,王磊,文西年.腺苷促进溃疡性结肠炎肠黏膜修复的实验研究[J].局解手术学杂志,2026,35(03):199-204. DOI： 10.11659/jjssx.06E025025.

HUANG He,WANG Lei,WEN Xi-nian.Experimental study on adenosine in promoting intestinal mucosal repair in ulcerative colitis[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(03):199-204. DOI： 10.11659/jjssx.06E025025.

摘要

目的

探究腺苷对溃疡性结肠炎（UC）大鼠肠黏膜损伤的影响及作用机制。

方法

以4%葡聚糖硫酸钠诱导建立UC大鼠模型，将40只SD大鼠随机分为对照组（饮用蒸馏水）、模型组（建立UC模型）、腺苷组（建模后腹腔注射5 mg/kg腺苷）、BzATP+腺苷组（建模后腹腔注射5 mg/kg腺苷和5 mg/kg P2X7受体激动剂BzATP），每组10只。对各组大鼠进行总体疾病活动指数（DAI）评分与结肠黏膜损伤指数（CMDI）评分；HE染色观察各组大鼠结肠组织病理学损伤；ELISA法测定各组大鼠结肠组织中炎症因子IL-1β、IL-18水平，免疫组化染色和Western blot检测各组大鼠结肠组织中P2X7受体（P2X7R）/核苷酸结合寡聚化结构域样受体蛋白3（NLRP3）炎症通路及闭锁蛋白（Occludin）、闭锁小带蛋白-1（ZO-1）的表达变化。

结果

与模型组比较，腺苷组大鼠DAI评分和CMDI评分降低，结肠组织充血、水肿及炎症细胞浸润减轻，结肠组织中IL-1β和IL-18水平，P2X7R、NLRP3和半胱天冬氨酸蛋白酶-1（Caspase-1）表达降低，Occludin、ZO-1表达升高，差异均有统计学意义（

0.05）。与腺苷组比较，腺苷+BzATP组大鼠DAI评分和CMDI评分升高，结肠组织充血、水肿加重，结肠组织中IL-1β、IL-18水平及P2X7R、NLRP3和Caspase-1表达升高，Occludin、ZO-1表达降低，差异均有统计学意义（

0.05）。

结论

腺苷能够改善UC大鼠肠黏膜损伤并促进肠黏膜屏障修复，其作用机制可能与抑制P2X7R介导的炎症级联反应有关。

Abstract

Objective

To investigate the effect of adenosine on intestinal mucosal injury in rats with ulcerative colitis （UC） and its mechanism.

Methods

UC was induced in SD rats by administering 4% dextran sulfate sodium. Forty SD rats were randomly divided into the control group （drinking distilled water），model group （induced UC model），adenosine group （intraperitoneal injection of 5 mg/kg adenosine after modeling），and BzATP+adenosine group （intraperitoneal injection of 5 mg/kg adenosine and 5 mg/kg of the P2X7 receptor agonist BzATP after modeling），with 10 rats in each group. The overall disease activity index （DAI） and colon mucosal injury index （CMDI） scores were assessed on the rats in each group； the colon tissue pathological damage of the rats in each group was observed by HE staining. The levels of inflammatory factors IL-1β and IL-18 in the colon tissue of the rats in each group were determined by ELISA，and the expression changes of P2X7 receptor （P2X7R）/NLR family pyrin domain containing 3 （NLRP3） inflammatory pathway，and Occludin and zonula occluden-1 （ZO-1） in the colon tissue of the rats in each group were detected by immunohistochemistry staining and Western blot.

Results

Compared with the model group，rats in the adenosine group showed significantly lower DAI and CMDI scores （

0.05），alleviated colon tissue congestion，edema，and inflammatory cell infiltration，decreased levels of IL-1β and IL-18，reduced expression of P2X7R，NLRP3，and Caspase-1，and increased expression of Occludin and ZO-1，with statistically significant differences （

0.05）. Conversely，compared to the adenosine group，the adenosine+BzATP group exhibited significantly higher DAI and CMDI scores，aggravated colon tissue congestion and edema，increased levels of IL-1β and IL-18，elevated expression of P2X7R，NLRP3，and Caspase-1，and decreased expression of Occludin and ZO-1，with statistically significant differences （

0.05）.

Conclusion

Adenosine can ameliorate intestinal mucosal injury and promote mucosal barrier repair in UC rats. This protective effect may be associated with the inhibition of the P2X7R-mediated inflammatory cascade.

关键词

Keywords

references

Le Berre C ， Honap S ， Peyrin-Biroulet L . Ulcerative colitis ［J］. Lancet ， 2023 ， 402 （ 10401 ）： 571 - 584 . doi： 10.1016/S0140-6736（23）00966-2 http://dx.doi.org/10.1016/S0140-6736（23）00966-2 .

Krugliak Cleveland N ， Torres J ， Rubin DT . What does disease progression look like in ulcerative colitis，and how might it be prevented？［J］. Gastroenterology ， 2022 ， 162 （ 5 ）： 1396 - 1408 . doi： 10.1053/j.gastro.2022.01.023 http://dx.doi.org/10.1053/j.gastro.2022.01.023 .

D'Amico F ， Fasulo E ， Jairath V ， et al . Management and treatment optimization of patients with mild to moderate ulcerative colitis ［J］. Expert Rev Clin Immunol ， 2024 ， 20 （ 3 ）： 277 - 290 . doi： 10.1080/1744666X.2023.2298956 http://dx.doi.org/10.1080/1744666X.2023.2298956 .

Ochoa B ， McMahon L . Surgery for ulcerative colitis ［J］. Semin Pediatr Surg ， 2024 ， 33 （ 2 ）： 151404 . doi： 10.1016/j.sempedsurg.2024.151404 http://dx.doi.org/10.1016/j.sempedsurg.2024.151404 .

Leoncini G ， Ronchetti S ， Villanacci V . Mucosal barrier dysfunction and relapse in ulcerative colitis： is there more that meets the eye？［J］. Dig Liver Dis ， 2024 ， 56 （ 8 ）： 1420 . doi： 10.1016/j.dld.2024.05.012 http://dx.doi.org/10.1016/j.dld.2024.05.012 .

Franco R ， Lillo A ， Navarro G ， et al . The adenosine A 2A receptor is a therapeutic target in neurological，heart and oncogenic diseases ［J］. Expert Opin Ther Targets ， 2022 ， 26 （ 9 ）： 791 - 800 . doi： 10.1080/14728222.2022.2132924 http://dx.doi.org/10.1080/14728222.2022.2132924 .

王龙胜，张文欣，张洁，等 . 腺苷对肿瘤获得性免疫的抑制作用及干预策略［J］. 浙江大学学报（医学版）， 2023 ， 52 （ 5 ）： 567 - 577 . doi： 10.3724/zdxbyxb-2023-0391 http://dx.doi.org/10.3724/zdxbyxb-2023-0391 .

Ercan G ， Yigitturk G ， Erbas O . Therapeutic effect of adenosine on experimentally induced acute ulcerative colitis model in rats ［J］. Acta Cir Bras ， 2020 ， 34 （ 12 ）： e201901204 . doi： 10.1590/s0102-865020190120000004 http://dx.doi.org/10.1590/s0102-865020190120000004 .

祁燕，万春平，李小丝，等 . 溃结康对溃疡性结肠炎小鼠肠粘膜炎症因子及肠屏障功能相关蛋白的影响［J］. 中药药理与临床， 2017 ， 33 （ 6 ）： 120 - 124 . doi： 10.13412/j.cnki.zyyl.2017.06.033 http://dx.doi.org/10.13412/j.cnki.zyyl.2017.06.033 .

Deng Z ， Luo XM ， Liu J ， et al . Quorum sensing，biofilm，and intestinal mucosal barrier： involvement the role of probiotic ［J］. Front Cell Infect Microbiol ， 2020 ， 10 ： 538077 . doi： 10.3389/fcimb.2020.538077 http://dx.doi.org/10.3389/fcimb.2020.538077 .

Kuo WT ， Odenwald MA ， Turner JR ， et al . Tight junction proteins occludin and ZO-1 as regulators of epithelial proliferation and survival ［J］. Ann N Y Acad Sci ， 2022 ， 1514 （ 1 ）： 21 - 33 . doi： 10.1111/nyas. 14798 http://dx.doi.org/10.1111/nyas.14798 .

Saha K ， Subramenium Ganapathy A ， Wang A ， et al . Autophagy reduces the degradation and promotes membrane localization of occludin to enhance the intestinal epithelial tight junction barrier against paracellular macromolecule flux ［J］. J Crohns Colitis ， 2023 ， 17 （ 3 ）： 433 - 449 . doi： 10.1093/ecco-jcc/jjac152 http://dx.doi.org/10.1093/ecco-jcc/jjac152 .

Kuo WT ， Zuo L ， Odenwald MA ， et al . The tight junction protein ZO-1 is dispensable for barrier function but critical for effective mucosal repair ［J］. Gastroenterology ， 2021 ， 161 （ 6 ）： 1924 - 1939 . doi： 10.1053/j.gastro.2021.08.047 http://dx.doi.org/10.1053/j.gastro.2021.08.047 .

Zeng M ， Feng A ， Zhao C ， et al . Adenosine ameliorated Aβ 25-35 -induced brain injury through the inhibition of apoptosis and oxidative stress via an ERα pathway ［J］. Brain Res ， 2022 ， 1788 ： 147944 . doi： 10.1016/j.brainres.2022.147944 http://dx.doi.org/10.1016/j.brainres.2022.147944 .

Leiva A ， Guzmán-Gutiérrez E ， Contreras-Duarte S ， et al . Adenosine receptors： modulators of lipid availability that are controlled by lipid levels ［J］. Mol Aspects Med ， 2017 ， 55 ： 26 - 44 . doi： 10.1016/j.mam.2017.01.001 http://dx.doi.org/10.1016/j.mam.2017.01.001 .

Stepanova M ， Aherne CM . Adenosine in intestinal epithelial barrier function ［J］. Cells ， 2024 ， 13 （ 5 ）： 381 . doi： 10.3390/cells13050381 http://dx.doi.org/10.3390/cells13050381 .

颜诗怡，陈诗垚，关飞，等 . ATP-P2X7R轴在炎症性疾病中的作用［J］. 热带病与寄生虫学， 2022 ， 20 （ 1 ）： 50 - 56 . doi： 10.3969/j.issn.1672-2302.2022.01.012 http://dx.doi.org/10.3969/j.issn.1672-2302.2022.01.012 .

Cheng N ， Zhang L ， Liu L . Understanding the role of purinergic P2X7 receptors in the gastrointestinal system： a systematic review ［J］. Front Pharmacol ， 2021 ， 12 ： 786579 . doi： 10.3389/fphar.2021.786579 http://dx.doi.org/10.3389/fphar.2021.786579 .

Jiang ZF ， Wu W ， Hu HB ， et al . P2X7 receptor as the regulator of T-cell function in intestinal barrier disruption ［J］. World J Gastroenterol ， 2022 ， 28 （ 36 ）： 5265 - 5279 . doi： 10.3748/wjg.v28.i36.5265 http://dx.doi.org/10.3748/wjg.v28.i36.5265 .

段晓鑫，黄深，杨桃，等 . 安肠汤调控P2X7R/NF-κB/NLRP3/GSDMD通路对溃疡性结肠炎大鼠炎症和细胞焦亡的影响［J］. 时珍国医国药， 2025 ， 36 （ 23 ）： 4429 - 4435 .

Rotondo JC ， Mazziotta C ， Lanzillotti C ， et al . The role of purinergic P2X7 receptor in inflammation and cancer： novel molecular insights and clinical applications ［J］. Cancers （Basel）， 2022 ， 14 （ 5 ）： 1116 . doi： 10.3390/cancers14051116 http://dx.doi.org/10.3390/cancers14051116 .

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