Experimental study on the transplantation of OECs/VitroGel™ 3D-RGD composite in the treatment of spinal cord injury in New Zealand rabbits

WU Guan-yin; LAN Shun-bao; JIANG Zhe-qi; DING Xu-qiu; CUI Xiao-ping; CHEN Jian-mei

doi:10.11659/jjssx.05E026029

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Experimental study on the transplantation of OECs/VitroGel™ 3D-RGD composite in the treatment of spinal cord injury in New Zealand rabbits

Basic Research | 更新时间：2026-10-26

- Experimental study on the transplantation of OECs/VitroGel™ 3D-RGD composite in the treatment of spinal cord injury in New Zealand rabbits
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 35, Issue 10, Pages: 867-872(2026)
- 作者机构：
  
  1.福建医科大学福总临床医学院/联勤保障部队第九〇〇医院骨科，福建福州 350000
  2.福建中医药大学中西医结合学院，福建福州 350000
  3.福建医科大学福总临床医学院/联勤保障部队第九〇〇医院神经内科，福建福州 350000
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.05E026029
  CLC： R651.2
- Received：08 May 2026，
  
  Revised：2026-09-17，
  
  Accepted：07 July 2026，
  
  Published：25 October 2026
- 稿件说明：
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吴官银,蓝顺宝,江哲琪等.OECs/VitroGel™ 3D-RGD复合物移植治疗新西兰兔脊髓损伤的实验研究[J].局解手术学杂志,2026,35(10):867-872.

WU Guan-yin,LAN Shun-bao,JIANG Zhe-qi,et al.Experimental study on the transplantation of OECs/VitroGel™ 3D-RGD composite in the treatment of spinal cord injury in New Zealand rabbits[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(10):867-872.
吴官银,蓝顺宝,江哲琪等.OECs/VitroGel™ 3D-RGD复合物移植治疗新西兰兔脊髓损伤的实验研究[J].局解手术学杂志,2026,35(10):867-872. DOI： 10.11659/jjssx.05E026029.

WU Guan-yin,LAN Shun-bao,JIANG Zhe-qi,et al.Experimental study on the transplantation of OECs/VitroGel™ 3D-RGD composite in the treatment of spinal cord injury in New Zealand rabbits[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2026,35(10):867-872. DOI： 10.11659/jjssx.05E026029.

摘要

目的

探讨嗅鞘细胞（OECs）/VitroGel™ 3D-RGD水凝胶复合物移植对新西兰兔脊髓损伤修复的影响。

方法

75只新西兰兔随机分为空白对照组、模型组、单纯水凝胶组、OECs组和复合物组，每组15只。除空白对照组外，模型组、单纯水凝胶组、OECs组和复合物组均建立T

脊髓损伤模型（重物打击致挫伤），并分别于损伤区注射PBS、水凝胶、OECs悬液或OECs/水凝胶复合物。于术后1、2、4、8、12周采用改良Tarlov评分评估实验兔后肢运动功能；采用苏木精-伊红（HE）染色测量脊髓组织空洞面积百分比；神经丝蛋白-200（NF-200）/胶质纤维酸性蛋白（GFAP）评价轴突再生和胶质瘢痕形成情况；分化簇68（CD68）/白细胞介素-10（IL-10）免疫荧光检测炎症细胞浸润和抗炎因子表达。

结果

复合物组实验兔术后12周的Tarlov评分高于模型组、单纯水凝胶组和OECs组（

0.01），脊髓组织空洞面积百分比低于模型组、单纯水凝胶组和OECs组（

0.01）。复合物组脊髓组织NF-200阳性轴突数多于单纯水凝胶组和OECs组（

0.01），GFAP阳性瘢痕面积低于单纯水凝胶组和OECs组（

0.01）。复合物组脊髓组织CD68阳性细胞密度低于单纯水凝胶组和OECs组（

0.01）。复合物组脊髓组织IL-10表达水平高于单纯水凝胶组和OECs组（

0.01）。

结论

OECs/VitroGel™ 3D-RGD复合物较单一细胞或材料移植更能促进兔脊髓损伤后结构修复、轴突再生和运动功能恢复，其作用可能与三维支架支持、营养因子分泌及炎症微环境改善有关。

Abstract

Objective

To investigate the effects of transplantation of olfactory ensheathing cells （OECs）/VitroGel™ 3D-RGD hydrogel composite on repair at 12 weeks after spinal cord injury in New Zealand rabbits.

Methods

A total of 75 New Zealand rabbits were randomly divided into the blank control group，the model group，the hydrogel-only group， the OECs group， and the composite group， with 15 rabbits in each group. Except for the blank control group， rabbits in the model group，the hydrogel-only group， the OECs group， and the composite group all underwent establishment of a T

spinal cord injury model by weight-drop contusion and received local injections of PBS， hydrogel， OECs suspension， or OECs/hydrogel composite， respectively. Hindlimb motor function o

f the rabbits was assessed by the modified Tarlov score at 1， 2， 4， 8， and 12 weeks after surgery. Hematoxylin-eosin staining was used to measure the percentage of spinal cord cavity area. Neurofilament-200（NF-200）/glial fibrillary acidic protein（GFAP） was used to assess axonal regeneration and glial scar formation， and cluster of differentiation 68（CD68）/interleukin-10（IL-10） immunofluorescence was used to detect inflammatory cell infiltration and anti-inflammatory factor expression.

Results

At 12 weeks after surgery， the composite group had higher Tarlov scores and lower percentage of spinal cord cavity area compared with the model group，the hydrogel-only group， and the OECs group （

0.01）. The composite group had more NF-200-positive axons and less GFAP-positive area in spinal cord tissue compared with the hydrogel-only group and the OECs group（

0.01）.The composite group showed lower CD68-positive cell density and higher mean optical density of IL-10 in spinal cord tissue compared with the hydrogel-only group and the OECs group（

0.01）.

Conclusion

OECs/VitroGel™ 3D-RGD composite transplantation is more effective in promoting tissue repair， axonal regeneration， and motor function recovery after spinal cord injury in rabbits than transplantation of cells or hydrogel alone. These effects may be related to three-dimensional scaffold support， neurotrophic factor secretion， and improvement of the inflammatory microenvironment.

关键词

Keywords

references

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