Mechanism of circ-0058063 on ferroptosis in esophageal cancer cells

HU Dong-yu; LI Hui; YANG Dong; LIU Hai-ying; GU Zhen-fang

doi:10.11659/jjssx.05E024125

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Mechanism of circ-0058063 on ferroptosis in esophageal cancer cells

Basic Research | 更新时间：2025-04-17

- Mechanism of circ-0058063 on ferroptosis in esophageal cancer cells
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 34, Issue 4, Pages: 289-294(2025)
- 作者机构：
  
  济宁医学院附属医院肿瘤科，山东济宁 272029
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.05E024125
  CLC： R735.1
- Received：27 May 2024，
  
  Published：25 April 2025
- 稿件说明：
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胡东玉,李慧,杨东等.circ-0058063对食管癌细胞铁死亡作用机制的研究[J].局解手术学杂志,2025,34(04):289-294.

HU Dong-yu,LI Hui,YANG Dong,et al.Mechanism of circ-0058063 on ferroptosis in esophageal cancer cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(04):289-294.
胡东玉,李慧,杨东等.circ-0058063对食管癌细胞铁死亡作用机制的研究[J].局解手术学杂志,2025,34(04):289-294. DOI： 10.11659/jjssx.05E024125.

HU Dong-yu,LI Hui,YANG Dong,et al.Mechanism of circ-0058063 on ferroptosis in esophageal cancer cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(04):289-294. DOI： 10.11659/jjssx.05E024125.

摘要

目的

探究circ-0058063调控ERK/MAPK信号通路对食管癌细胞铁死亡的作用机制。

方法

将EC9706细胞随机分为Control组、si-NC组、si-circ-0058063组和si-circ-0058063+ISO组。qRT-PCR检测食管癌组织及细胞中circ-0058063表达；CCK-8和克隆形成实验检测细胞增殖能力；检测细胞中Fe

、丙二醛（MDA）、谷胱甘肽（GSH）和活性氧（ROS）水平；Western blot检测细胞中ERK/MAPK信号通路及铁死亡相关蛋白表达。

结果

与癌旁组织相比，食管癌组织中circ-0058063表达水平明显升高（

0.05）；人食管癌细胞EC9706中circ-0058063表达水平明显高于人正常食管上皮细胞HEEC（

0.05）。与Control组相比，si-circ-0058063组细胞中circ-0058063表达水平、细胞存活率、克隆细胞数、GSH水平、p-ERK1/2/ERK1/2和p-p38MAPK/p38MAPK比值及SLC7A11、GPX4蛋白表达水平均明显降低（

0.05），细胞中Fe

、MDA及ROS水平均明显增加（

0.05）；与si-circ-0058063组相比，si-circ-0058063+ISO组细胞中circ-0058063表达水平、细胞存活率、克隆细胞数、GSH水平、p-ERK1/2/ERK1/2和p-p38MAPK/p38MAPK比值及SLC7A11、GPX4蛋白表达水平均明显升高（

0.05），Fe

、MDA及ROS水平均明显降低（

0.05）。

结论

敲减circ-0058063可抑制食管癌细胞增殖，诱导食管癌细胞铁死亡，其作用机制可能与抑制ERK/MAPK信号通路激活有关。

Abstract

Objective

To investigate the mechanism of action of circ-0058063 regulating ERK/MAPK signaling pathway on ferroptosis in esophageal cancer cells.

Methods

EC9706 cells were randomly divided into the Control group， the si-NC group， the si-circ-0058063 group， and the si-circ-0058063+ISO

group. qRT-PCR was used to detect the expression of circ-0058063 in esophageal cancer tissues and cells； CCK-8 and clone formation assay were used to detect the cell proliferation ability. The levels of Fe

， malondialdehyde （MDA）， glutathione （GSH）， and reactive oxygen species （ROS） in cells were detected. Western blot was used to detect the ERK/MAPK signaling pathway and the expression of ferroptosis-related protein in cells.

Results

The expression level of circ-0058063 in esophageal cancer tissue was significantly higher than that in adjacent tissues （

0.05）； the expression level of circ-0058063 in human esophageal cancer cell EC9706 was significantly higher than that in human normal esophageal epithelial cell HEEC （

0.05）. Compared with the Control group， the expression level of circ-0058063 in cells， cell survival rate， number of cloned cells， GSH level， ratios of p-ERK1/2/ERK1/2 and p-p38MAPK/p38MAPK， as well as the expression levels of SLC7A11 and GPX4 protein in the si-circ-0058063 group were significantly reduced （

0.05）， while the levels of Fe

， MDA， and ROS in cells were significantly increased （

0.05）. Compared with the si-circ-0058063 group， the expression level of circ-0058063 in cells， cell survival rate， number of cloned cells， GSH level， ratios of p-ERK1/2/ERK1/2 and p-p38MAPK/p38MAPK， as well as the expression levels of SLC7A11 and GPX4 protein in the si-circ-0058063+ISO group were significantly increased （

0.05）， while the levels of Fe

， MDA， and ROS in cells were significantly decreased （

0.05）.

Conclusion

Knockdown of circ-0058063 can inhibit the proliferation of esophageal cancer cells and induce ferroptosis， and its mechanism of action may be related to the inhibition of ERK/MAPK signaling pathway activation.

关键词

Keywords

references

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