Study on the mechanism of different concentrations of simvastatin on regeneration of sciatic nerve injury in rats

LI Yun-hu; CAO Jun-wei; LI Chen; ZHANG Jing-yu; DUN Ai-she; WANG Hong-bin

doi:10.11659/jjssx.05E024089

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Study on the mechanism of different concentrations of simvastatin on regeneration of sciatic nerve injury in rats

Basic Research | 更新时间：2025-09-24

- Study on the mechanism of different concentrations of simvastatin on regeneration of sciatic nerve injury in rats
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 34, Issue 9, Pages: 772-775(2025)
- 作者机构：
  
  1.山东第一医科大学口腔医学院2021级口腔医学本科专业，山东济南250117
  2.山东第一医科大学口腔医学院口腔基础医学教研室，山东济南250117
  3.山东第一医科大学临床与基础医学院人体解剖学教研室，山东济南250117
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.05E024089
  CLC： R745.4
- Received：20 May 2024，
  
  Published：25 September 2025
- 稿件说明：
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李云虎,曹峻玮,李晨等.不同浓度辛伐他汀对大鼠坐骨神经损伤后再生作用的研究[J].局解手术学杂志,2025,34(09):772-775.

LI Yun-hu,CAO Jun-wei,LI Chen,et al.Study on the mechanism of different concentrations of simvastatin on regeneration of sciatic nerve injury in rats[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(09):772-775.
李云虎,曹峻玮,李晨等.不同浓度辛伐他汀对大鼠坐骨神经损伤后再生作用的研究[J].局解手术学杂志,2025,34(09):772-775. DOI： 10.11659/jjssx.05E024089.

LI Yun-hu,CAO Jun-wei,LI Chen,et al.Study on the mechanism of different concentrations of simvastatin on regeneration of sciatic nerve injury in rats[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(09):772-775. DOI： 10.11659/jjssx.05E024089.

摘要

目的

探究不同浓度辛伐他汀对坐骨神经损伤后神经再生的影响。

方法

将大鼠随机分为正常组、对照组、低剂量组、高剂量组，每组3只。除正常组外，其他组均建立成年大鼠坐骨神经挤压损伤模型。正常组和对照组大鼠口饲清水，低剂量组和高剂量组大鼠分别口饲4 mg/mL、40 mg/mL的98%辛伐他汀。采用坐骨神经功能指数（SFI）、HE染色、劳克坚牢蓝（LFB）染色及免疫荧光染色等评估大鼠坐骨神经再生情况。

结果

高剂量组大鼠术后7 d、14 d的SFI高于对照组（

0.05）；低剂量组与对照组大鼠术后7 d、14 d的SFI比较，差异无统计学意义（

0.05）。HE染色及LFB染色结果显示：与对照组相比，高剂量组大鼠神经元数量增多，神经纤维及髓鞘更为清晰和密集，神经功能明显恢复；而低剂量组大鼠的坐骨神经则未见明显改善。免疫荧光染色结果显示：与对照组相比，高剂量组免疫荧光强度增高，而低剂量组免疫荧光强度降低，差异均有统计学意义（

0.05）。

结论

高剂量辛伐他汀可通过调控M2型巨噬细胞的表达，促进周围神经再生。

Abstract

Objective

To explore the effects of different concentrations of simvastatin on nerve regeneration after sciatic nerve injury.

Methods

Rats were randomly divided into the normal group， the control group， the low-dose group and the high-dose group， with 3 rats in each group. Except for the normal group， adult rat sciatic nerve crush injury models were established in the other groups. Rats in the normal group and the control group were orally administered with water，while those in the low-dose group and high-dose group were orally administered with 98% simvastatin at dosages of 4 mg/mL and 40 mg/mL，respectively. The sciatic nerve regeneration in rats was evaluated by sciatic function index （SFI）， HE staining， luxol fast blue（LFB） staining and immunofluorescence staining， etc.

Results

The SFI of rats in the high-dose group 7 days and 14 days after surgery were higher than those in the control group （

0.05）； there was no significant difference in SFI of rats between the low-dose group and the control group 7 days and 14 days after surgery（

0.05）.HE staining and L

FB staining results showed that compared with the control group，the number of neurons of rats in the high-dose group increased，the nerve fibers and myelin were clearer and denser，and the nerve function was significantly restored； while no significant improvement was observed in the sciatic nerve of rats in the low-dose group.The immunofluorescence staining results showed that compared with the control group，the immunofluorescence intensity in the high-dose group increased， while that in the low-dose group decreased，the differences were statistically significant （

0.05）.

Conclusion

High-dose simvastatin can promote peripheral nerve regeneration by regulating the expression of M2 macrophages.

关键词

Keywords

references

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