Expression and role of Arginase Ⅱ in the kidney tissues of rats with type 2 diabetic nephropathy

LI Xiu; ZHANG Hai-ying; JIANG Yu-bo; WANG Shao-qing; MO Zi-yi; XUE Shi-yuan; LIU Chang

doi:10.11659/jjssx.05E024062

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Expression and role of Arginase Ⅱ in the kidney tissues of rats with type 2 diabetic nephropathy

Basic Research | 更新时间：2025-03-25

- Expression and role of Arginase Ⅱ in the kidney tissues of rats with type 2 diabetic nephropathy
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 34, Issue 3, Pages: 205-211(2025)
- 作者机构：
  
  1.成都医学院基础医学院，四川成都 610500
  2.成都市郫都区人民医院内分泌代谢科，四川成都 611730
  3.成都医学院第二附属医院肾内科，四川成都 610057
  4.成都医学院药学院，四川成都 610500
  5.成都医学院临床医学院，四川成都 610500
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.05E024062
  CLC： R364.5
- Received：13 May 2024，
  
  Published：25 March 2025
- 稿件说明：
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李秀,张海英,江玉波等.精氨酸酶Ⅱ在2型糖尿病肾病大鼠肾组织中的表达及作用[J].局解手术学杂志,2025,34(03):205-211.

LI Xiu,ZHANG Hai-ying,JIANG Yu-bo,et al.Expression and role of Arginase Ⅱ in the kidney tissues of rats with type 2 diabetic nephropathy[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(03):205-211.
李秀,张海英,江玉波等.精氨酸酶Ⅱ在2型糖尿病肾病大鼠肾组织中的表达及作用[J].局解手术学杂志,2025,34(03):205-211. DOI： 10.11659/jjssx.05E024062.

LI Xiu,ZHANG Hai-ying,JIANG Yu-bo,et al.Expression and role of Arginase Ⅱ in the kidney tissues of rats with type 2 diabetic nephropathy[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2025,34(03):205-211. DOI： 10.11659/jjssx.05E024062.

摘要

目的

探讨精氨酸酶Ⅱ（ArgⅡ）在糖尿病肾病（DN）大鼠肾组织中的表达及其在DN发生过程中的作用。

方法

10只雄性SD大鼠随机分为对照组和模型组，每组5只。采用高糖高脂饲料饲养和腹腔注射小剂量链脲佐菌素（45 mg/kg）制作大鼠DN模型，继续饲养11周后处死。测定大鼠体质量和血、尿生化指标；取右肾称重并进行病理学检查，观察肾组织病理学变化和ArgⅡ、CD68

蛋白表达，免疫荧光双染色法观察ArgⅡ与肾巨噬细胞活化标志因子CD68

的分布和表达情况；Western blot检测肾组织中ArgⅡ、NF-κB、TNF-α、IL-6蛋白表达情况。

结果

与对照组相比，模型组肾质量/体质量、24 h尿量、24 h尿蛋白、空腹血糖、尿素氮和胰岛素水平均明显增高（

0.05）。肾组织病理学结果显示：模型组大鼠肾小球系膜细胞坏死，血管扩张，肾小管上皮细胞脂肪变性、淤血。与对照组比较，模型组大鼠肾组织ArgⅡ、CD68

、NF-κB、TNF-α、IL-6蛋白表达均明显升高（

0.05）。免疫荧光双染色显示，ArgⅡ和CD68

共表达于肾组织中，且模型组大鼠ArgⅡ、CD68

荧光强度强于对照组（

0.01）。

结论

ArgⅡ在DN中表达增高，其可能参与了DN炎性病变的发生。

Abstract

Objective

To investigate the expression of arginase Ⅱ（ArgⅡ）in kidney tissue of rats with diabetic nephropathy（DN）and its significance in the development of DN.

Methods

A total of 10 male SD rats were randomly divided into the control group an

d the model group ，with 5 rats in each group. An rat model of DN was developed by feeding with high-sugar and high-fat diet combined with intraperitoneal injection of low-dose streptozotocin （45 mg/kg），and they were sacrificed after 11 weeks of continued feeding.The body weight，and biochemical indexes of blood and urine of rats were determined.The right kidney was weighed and histopathological examination was performed. The pathological changes of kidney tissues and protein expression of ArgⅡ and CD68

were observed，and the immunofluorescence double staining was used to observe the distribution and expression of ArgⅡand a marker of renal macrophage activation CD68

； the protein expression of ArgⅡ，NF-κB，TNF-α and IL-6 in kidney tissues was determined by Western blot.

Results

Compared with the control group，the ratio of kidney weight to body weight，24-hour urine volume，24-hour urine protein，fasting blood glucose，urea nitrogen and insulin level in the model group were significantly increased（

0.05）. The renal histopathology showed that the mesangial cells of the renal glomerular were necrotic with vascular dilatation，and the renal tubular epithelial cells were steatosis and congestion. Compared with the control group，the protein expression of ArgⅡ，CD68

，NF-κB，TNF-α and IL-6 in the kidney tissues of the model group were significantly increased （

0.05）. Immunofluorescence double staining demonstrated the co-expression of ArgⅡ and CD68

in renal tissue，and the fluorescence intensities of both ArgⅡ and CD68

in the model group were significantly stronger than those in the control group （

0.01）.

Conclusion

The expression of ArgⅡ is increased in DN，which may be participated in the occurrence of inflammatory lesions in DN.

关键词

Keywords

references

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